Sprouty 4 expression in human oral squamous cell carcinogenesis
Background/purpose: Reviewing literature, sprouty 4 (SPRY4) has not been studied in human oral squamous cell carcinomas (OSCCs). The study aimed to examine SPRY4 expression in human oral squamous cell carcinogenesis. Materials and methods: A total of 95 OSCCs, 10 OPMDs with malignant transformation...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Journal of Dental Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790223000168 |
| _version_ | 1797862207579488256 |
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| author | Pei-Hsien Liao Fu-Hsiung Chuang Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen |
| author_facet | Pei-Hsien Liao Fu-Hsiung Chuang Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen |
| author_sort | Pei-Hsien Liao |
| collection | DOAJ |
| description | Background/purpose: Reviewing literature, sprouty 4 (SPRY4) has not been studied in human oral squamous cell carcinomas (OSCCs). The study aimed to examine SPRY4 expression in human oral squamous cell carcinogenesis. Materials and methods: A total of 95 OSCCs, 10 OPMDs with malignant transformation (MT), 17 OPMDs without MT, and six normal oral mucosa (NOM) samples were recruited for immunohistochemical staining; three OSCC tissues with normal tissue counterpart NOM were employed for Western blotting. Three human oral cancer cell lines (OCCLs), an oral precancer cell line (dysplastic oral keratinocyte, DOK), and a primary culture of normal oral keratinocytes (HOK) were used for Western blotting; OCCLs and HOK were employed for real-time quantitative reverse transcription-polymerase chain reaction. OCCLs were evaluated in terms of proliferation, migration, and invasion assays. Results: SPRY4 protein expression was significantly increased in OSCCs compared with NOM. Protein and mRNA SPRY4 expression in OCCLs were significantly elevated compared with HOK. Significant increases in the degrees of proliferation, migration, and invasion were noted in OCCLs with SPRY4 siRNA transfection compared with those without transfection. SPRY4 protein level was increased in OPMD with MT compared to OPMD without MT. SPRY4 protein was significant increase in DOK in comparison with HOK. SPRY4 protein expression was significantly increased from NOM and OPMD without MT to OSCC. SPRY4 protein expression in OCCLs was significantly enhanced compared with DOK and HOK respectively. Conclusion: Our results indicate that SPRY4 expression is possibly involved in human oral squamous cell carcinogenesis. |
| first_indexed | 2024-04-09T22:15:31Z |
| format | Article |
| id | doaj.art-cc3fa68ca6f94d62a7cbd4b252da46c1 |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-04-09T22:15:31Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-cc3fa68ca6f94d62a7cbd4b252da46c12023-03-23T04:35:21ZengElsevierJournal of Dental Sciences1991-79022023-04-01182781790Sprouty 4 expression in human oral squamous cell carcinogenesisPei-Hsien Liao0Fu-Hsiung Chuang1Yen-Yun Wang2Wen-Chen Wang3Chiang-Wei Su4Ching-Wei Hsu5Shyng-Shiou Yuan6Yuk-Kwan Chen7School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Endodontics and Operative Dentistry, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, TaiwanSchool of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Oral Pathology & Maxillofacial Radiology, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Oral & Maxillofacial Imaging Center, Kaohsiung Medical University, Kaohsiung, TaiwanDivision of Oral & Maxillofacial Surgery, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDivision of Oral & Maxillofacial Surgery, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, TaiwanDepartment of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Obstetrics and Gynecology and Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author. Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan.School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Oral Pathology & Maxillofacial Radiology, Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Oral & Maxillofacial Imaging Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Corresponding author. School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan.Background/purpose: Reviewing literature, sprouty 4 (SPRY4) has not been studied in human oral squamous cell carcinomas (OSCCs). The study aimed to examine SPRY4 expression in human oral squamous cell carcinogenesis. Materials and methods: A total of 95 OSCCs, 10 OPMDs with malignant transformation (MT), 17 OPMDs without MT, and six normal oral mucosa (NOM) samples were recruited for immunohistochemical staining; three OSCC tissues with normal tissue counterpart NOM were employed for Western blotting. Three human oral cancer cell lines (OCCLs), an oral precancer cell line (dysplastic oral keratinocyte, DOK), and a primary culture of normal oral keratinocytes (HOK) were used for Western blotting; OCCLs and HOK were employed for real-time quantitative reverse transcription-polymerase chain reaction. OCCLs were evaluated in terms of proliferation, migration, and invasion assays. Results: SPRY4 protein expression was significantly increased in OSCCs compared with NOM. Protein and mRNA SPRY4 expression in OCCLs were significantly elevated compared with HOK. Significant increases in the degrees of proliferation, migration, and invasion were noted in OCCLs with SPRY4 siRNA transfection compared with those without transfection. SPRY4 protein level was increased in OPMD with MT compared to OPMD without MT. SPRY4 protein was significant increase in DOK in comparison with HOK. SPRY4 protein expression was significantly increased from NOM and OPMD without MT to OSCC. SPRY4 protein expression in OCCLs was significantly enhanced compared with DOK and HOK respectively. Conclusion: Our results indicate that SPRY4 expression is possibly involved in human oral squamous cell carcinogenesis.http://www.sciencedirect.com/science/article/pii/S1991790223000168SPRY4Oral squamous cell carcinomaOral potentially malignant disorders |
| spellingShingle | Pei-Hsien Liao Fu-Hsiung Chuang Yen-Yun Wang Wen-Chen Wang Chiang-Wei Su Ching-Wei Hsu Shyng-Shiou Yuan Yuk-Kwan Chen Sprouty 4 expression in human oral squamous cell carcinogenesis Journal of Dental Sciences SPRY4 Oral squamous cell carcinoma Oral potentially malignant disorders |
| title | Sprouty 4 expression in human oral squamous cell carcinogenesis |
| title_full | Sprouty 4 expression in human oral squamous cell carcinogenesis |
| title_fullStr | Sprouty 4 expression in human oral squamous cell carcinogenesis |
| title_full_unstemmed | Sprouty 4 expression in human oral squamous cell carcinogenesis |
| title_short | Sprouty 4 expression in human oral squamous cell carcinogenesis |
| title_sort | sprouty 4 expression in human oral squamous cell carcinogenesis |
| topic | SPRY4 Oral squamous cell carcinoma Oral potentially malignant disorders |
| url | http://www.sciencedirect.com/science/article/pii/S1991790223000168 |
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