Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers
The crude polysaccharide extracted from the flowers of burley tobacco hybrid Eyan 1 was fractionated on a Sepharose CL-6B chromatography to get two purified polysaccharide fractions (termed Fr-I and Fr-II). The monocarbohydrate compositions revealed both fractions were heteropolysaccharide. Fr-I was...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek
2013-01-01
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| Series: | Croatian Journal of Food Science and Technology |
| Online Access: | http://hrcak.srce.hr/file/168087 |
| _version_ | 1819002287064875008 |
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| author | Y. Xiao D. B. Mao Chun-Ping Xu |
| author_facet | Y. Xiao D. B. Mao Chun-Ping Xu |
| author_sort | Y. Xiao |
| collection | DOAJ |
| description | The crude polysaccharide extracted from the flowers of burley tobacco hybrid Eyan 1 was fractionated on a Sepharose CL-6B chromatography to get two purified polysaccharide fractions (termed Fr-I and Fr-II). The monocarbohydrate compositions revealed both fractions were heteropolysaccharide. Fr-I was mainly composed of mannose, galactose, ribose and arabinose, while Fr-II was composed of mannose, galactose and glucose. The IR spectrum of Fr-I and Fr-II revealed the typical characteristics with β-configuration. Furthermore, TGA (Thermogravimetric analysis) indicated the degradation temperature (Td) of the Fr-I and Fr-II was 210 °C and 185 °C, respectively. Both Fr-I and Fr-II possessed good scavenging abilities on hydroxyl radicals (IC50 8.26 mg/ml and 6.23 mg/ml, respectively) and DPPH radicals (IC50 20.3±1.20 µg/ml, 17.5±1.28 µg/ml, respectively). Fr-II showed higher antioxidant properties than Fr-I (p < 0.05). In a 13C NMR analysis of Fr-II, three anomeric peaks appeared at 102.95, 95.79 and 91.85 ppm, which were assigned to the mannose (C-1), galactose (C-1) and glucose (C-1) residues, respectively. |
| first_indexed | 2024-12-20T23:02:42Z |
| format | Article |
| id | doaj.art-cc41109e8a524de9a8acda4f16c937c8 |
| institution | Directory Open Access Journal |
| issn | 1847-3466 1848-9923 |
| language | English |
| last_indexed | 2024-12-20T23:02:42Z |
| publishDate | 2013-01-01 |
| publisher | Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek |
| record_format | Article |
| series | Croatian Journal of Food Science and Technology |
| spelling | doaj.art-cc41109e8a524de9a8acda4f16c937c82022-12-21T19:23:57ZengJosip Juraj Strossmayer University of Osijek, Faculty of Food Technology OsijekCroatian Journal of Food Science and Technology1847-34661848-99232013-01-01524652113687Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowersY. Xiao0D. B. Mao1Chun-Ping Xu2College of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R. ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R. ChinaCollege of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, Henan, 450002, P.R. ChinaThe crude polysaccharide extracted from the flowers of burley tobacco hybrid Eyan 1 was fractionated on a Sepharose CL-6B chromatography to get two purified polysaccharide fractions (termed Fr-I and Fr-II). The monocarbohydrate compositions revealed both fractions were heteropolysaccharide. Fr-I was mainly composed of mannose, galactose, ribose and arabinose, while Fr-II was composed of mannose, galactose and glucose. The IR spectrum of Fr-I and Fr-II revealed the typical characteristics with β-configuration. Furthermore, TGA (Thermogravimetric analysis) indicated the degradation temperature (Td) of the Fr-I and Fr-II was 210 °C and 185 °C, respectively. Both Fr-I and Fr-II possessed good scavenging abilities on hydroxyl radicals (IC50 8.26 mg/ml and 6.23 mg/ml, respectively) and DPPH radicals (IC50 20.3±1.20 µg/ml, 17.5±1.28 µg/ml, respectively). Fr-II showed higher antioxidant properties than Fr-I (p < 0.05). In a 13C NMR analysis of Fr-II, three anomeric peaks appeared at 102.95, 95.79 and 91.85 ppm, which were assigned to the mannose (C-1), galactose (C-1) and glucose (C-1) residues, respectively.http://hrcak.srce.hr/file/168087 |
| spellingShingle | Y. Xiao D. B. Mao Chun-Ping Xu Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers Croatian Journal of Food Science and Technology |
| title | Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| title_full | Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| title_fullStr | Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| title_full_unstemmed | Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| title_short | Antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| title_sort | antioxidant activities of polysaccharide fractions isolated from burley tobacco flowers |
| url | http://hrcak.srce.hr/file/168087 |
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