Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling
This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (<i>n</i> = 8), where the lambs rema...
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MDPI AG
2021-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/9/2656 |
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| author | José Manuel Hernández-Hernández Graeme B. Martin Carlos Miguel Becerril-Pérez Arturo Pro-Martínez César Cortez-Romero Jaime Gallegos-Sánchez |
| author_facet | José Manuel Hernández-Hernández Graeme B. Martin Carlos Miguel Becerril-Pérez Arturo Pro-Martínez César Cortez-Romero Jaime Gallegos-Sánchez |
| author_sort | José Manuel Hernández-Hernández |
| collection | DOAJ |
| description | This study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (<i>n</i> = 8), where the lambs remained with their mothers; and (2) restricted suckling (<i>n</i> = 8), where the mothers suckled their lambs twice daily for 30 min. On Day 19 postpartum, the ewes were individually penned with <i>ad libitum</i> access to water and feed and given an indwelling catheter in each jugular vein. On Day 20, 4 mL of blood was sampled every 15 min from 08:00 to 20:00 h to determine LH pulse frequency. At 14:00 h, four ewes in each group received 120 μg of kisspeptin diluted in 3 mL of saline as a continuous infusion for 6 h; the remaining four ewes in each group received only saline. The interaction between kisspeptin and suckling type did not affect LH pulse frequency (<i>p</i> > 0.05). Before kisspeptin administration, pulse frequency was similar in all groups (1.50 ± 0.40 pulses per 6 h; <i>p</i> > 0.05). With the application of kisspeptin, pulse frequency increased to 3.50 ± 0.43 pulses per 6 h (<i>p</i> ≤ 0.014), so the concentration of LH (1.11 ± 0.14 ng mL<sup>−1</sup>) was greater in kisspeptin-treated ewes than in saline-treated ewes (0.724 ± 0.07 ng mL<sup>−1</sup>; <i>p</i> ≤ 0.040). The frequency of LH pulses was greater with restricted suckling than with continuous suckling (2.44 ± 0.29 versus 1.69 ± 0.29 pulses per 6 h; <i>p</i> ≤ 0.040). We conclude that intravenous application of kisspeptin increases the pulsatile secretion of LH in suckling ewes and that suckling might reduce kisspeptin neuronal activity, perhaps explaining the suppression of ovulation. Moreover, the effects of kisspeptin and suckling on pulsatile LH secretion appear to be independent, perhaps operating through different neural pathways. |
| first_indexed | 2024-03-10T07:58:28Z |
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| language | English |
| last_indexed | 2024-03-10T07:58:28Z |
| publishDate | 2021-09-01 |
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| spelling | doaj.art-cc454cd99ba741119a0eee7337f3372a2023-11-22T11:43:30ZengMDPI AGAnimals2076-26152021-09-01119265610.3390/ani11092656Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted SucklingJosé Manuel Hernández-Hernández0Graeme B. Martin1Carlos Miguel Becerril-Pérez2Arturo Pro-Martínez3César Cortez-Romero4Jaime Gallegos-Sánchez5Programa de Ganadería, Colegio de Postgraduados, Campus Montecillo, km 36.5 Carretera Federal México-Texcoco, Montecillo 56230, MexicoSchool of Agriculture and Environment, University of Western Australia, Crawley 6009, AustraliaColegio de Postgraduados, Campus Veracruz, Veracruz 91690, MexicoPrograma de Ganadería, Colegio de Postgraduados, Campus Montecillo, km 36.5 Carretera Federal México-Texcoco, Montecillo 56230, MexicoColegio de Postgraduados, Campus San Luis Potosí, San Luís Potosi 78600, MexicoPrograma de Ganadería, Colegio de Postgraduados, Campus Montecillo, km 36.5 Carretera Federal México-Texcoco, Montecillo 56230, MexicoThis study tested whether the intravenous application of kisspeptin can stimulate the pulsatile secretion of LH in suckling ewes during postpartum anestrus. Ten days after lambing, Pelibuey ewes were allocated among two groups: (1) continuous suckling (<i>n</i> = 8), where the lambs remained with their mothers; and (2) restricted suckling (<i>n</i> = 8), where the mothers suckled their lambs twice daily for 30 min. On Day 19 postpartum, the ewes were individually penned with <i>ad libitum</i> access to water and feed and given an indwelling catheter in each jugular vein. On Day 20, 4 mL of blood was sampled every 15 min from 08:00 to 20:00 h to determine LH pulse frequency. At 14:00 h, four ewes in each group received 120 μg of kisspeptin diluted in 3 mL of saline as a continuous infusion for 6 h; the remaining four ewes in each group received only saline. The interaction between kisspeptin and suckling type did not affect LH pulse frequency (<i>p</i> > 0.05). Before kisspeptin administration, pulse frequency was similar in all groups (1.50 ± 0.40 pulses per 6 h; <i>p</i> > 0.05). With the application of kisspeptin, pulse frequency increased to 3.50 ± 0.43 pulses per 6 h (<i>p</i> ≤ 0.014), so the concentration of LH (1.11 ± 0.14 ng mL<sup>−1</sup>) was greater in kisspeptin-treated ewes than in saline-treated ewes (0.724 ± 0.07 ng mL<sup>−1</sup>; <i>p</i> ≤ 0.040). The frequency of LH pulses was greater with restricted suckling than with continuous suckling (2.44 ± 0.29 versus 1.69 ± 0.29 pulses per 6 h; <i>p</i> ≤ 0.040). We conclude that intravenous application of kisspeptin increases the pulsatile secretion of LH in suckling ewes and that suckling might reduce kisspeptin neuronal activity, perhaps explaining the suppression of ovulation. Moreover, the effects of kisspeptin and suckling on pulsatile LH secretion appear to be independent, perhaps operating through different neural pathways.https://www.mdpi.com/2076-2615/11/9/2656kisspeptinLHrestricted sucklinganestrussheep |
| spellingShingle | José Manuel Hernández-Hernández Graeme B. Martin Carlos Miguel Becerril-Pérez Arturo Pro-Martínez César Cortez-Romero Jaime Gallegos-Sánchez Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling Animals kisspeptin LH restricted suckling anestrus sheep |
| title | Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling |
| title_full | Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling |
| title_fullStr | Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling |
| title_full_unstemmed | Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling |
| title_short | Kisspeptin Stimulates the Pulsatile Secretion of Luteinizing Hormone (LH) during Postpartum Anestrus in Ewes Undergoing Continuous and Restricted Suckling |
| title_sort | kisspeptin stimulates the pulsatile secretion of luteinizing hormone lh during postpartum anestrus in ewes undergoing continuous and restricted suckling |
| topic | kisspeptin LH restricted suckling anestrus sheep |
| url | https://www.mdpi.com/2076-2615/11/9/2656 |
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