Cytotoxicity of Two Bioactive Root Canal Sealers
Objective: The aim of this study was to investigate the cytotoxicity of two different bioactive root canal sealers: one based on mineral trioxide aggregate, MTA Fillapex (Angelus, Solucoes Odontologicas, Londrina, PR, Brazil), and the other based on bioceramics, Endosequence BC Sealer (Brasseler,...
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| Format: | Article |
| Language: | English |
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University of Zagreb. School of Dental Medicine
2016-01-01
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| Series: | Acta Stomatologica Croatica |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/227450 |
| _version_ | 1797208035504947200 |
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| author | Anja Baraba Sonja Pezelj-Ribarić Marija Roguljić Ivana Miletić |
| author_facet | Anja Baraba Sonja Pezelj-Ribarić Marija Roguljić Ivana Miletić |
| author_sort | Anja Baraba |
| collection | DOAJ |
| description | Objective: The aim of this study was to investigate the cytotoxicity of two different bioactive root canal sealers: one based on mineral trioxide aggregate, MTA Fillapex (Angelus, Solucoes Odontologicas,
Londrina, PR, Brazil), and the other based on bioceramics, Endosequence BC Sealer (Brasseler, Savannah, Georgia, USA), in culture of mouse L929 fibroblasts. Materials and methods: Mouse fibroblasts (L929), obtained from subcutaneous connective tissue of mouse line C3Hf, were cultivated in plastic culture flasks in an incubator at 37ºC, with 5% CO2 and 90% humidity. Freshly mixed Endosequence BC Sealer and MTA Fillapex (0.1 g each) were placed on sterile teflon discs, 6 mm in diameter. Teflon discs with the materials as well as empty discs serving as control were placed in wells of 12-well plate. After incubation times of 1, 6, 20 and 24 hours, the teflon discs were removed from the wells and the number of viable cells was determined using trypan blue in Neubauer chamber. Results: In comparison to the control group, MTA Fillapex had significantly less viable cells for all incubation periods (p≤0.05), while Endosequence BC sealer had significantly less viable cells after 6, 20,
and 24 hours of incubation (p≤0.05). MTA Fillapex exhibited ignificantly less viable cells in comparison to Endosequence BC sealer after the first hour and after 20 hours of incubation (p≤0.05), while for the other incubation periods there were no significant differences (p≥0.05). Conclusion: MTA Fillapex and Endosequence BC sealer were both cytotoxic in cultures of mouse L929 fibroblasts. |
| first_indexed | 2024-04-24T09:32:24Z |
| format | Article |
| id | doaj.art-cc469f5c376243c7a848dba2dcd77104 |
| institution | Directory Open Access Journal |
| issn | 0001-7019 1846-0410 |
| language | English |
| last_indexed | 2024-04-24T09:32:24Z |
| publishDate | 2016-01-01 |
| publisher | University of Zagreb. School of Dental Medicine |
| record_format | Article |
| series | Acta Stomatologica Croatica |
| spelling | doaj.art-cc469f5c376243c7a848dba2dcd771042024-04-15T13:46:44ZengUniversity of Zagreb. School of Dental MedicineActa Stomatologica Croatica0001-70191846-04102016-01-0150181310.15644/asc50/1/2Cytotoxicity of Two Bioactive Root Canal SealersAnja Baraba0Sonja Pezelj-Ribarić1Marija Roguljić2Ivana Miletić3Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, CroatiaChair of Oral Medicine and Periodontology, Clinical Hospital Centre, Faculty of Medicine, University of Rijeka, Rijeka, CroatiaDental Polyclinic Split, Split, CroatiaDepartment of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, CroatiaObjective: The aim of this study was to investigate the cytotoxicity of two different bioactive root canal sealers: one based on mineral trioxide aggregate, MTA Fillapex (Angelus, Solucoes Odontologicas, Londrina, PR, Brazil), and the other based on bioceramics, Endosequence BC Sealer (Brasseler, Savannah, Georgia, USA), in culture of mouse L929 fibroblasts. Materials and methods: Mouse fibroblasts (L929), obtained from subcutaneous connective tissue of mouse line C3Hf, were cultivated in plastic culture flasks in an incubator at 37ºC, with 5% CO2 and 90% humidity. Freshly mixed Endosequence BC Sealer and MTA Fillapex (0.1 g each) were placed on sterile teflon discs, 6 mm in diameter. Teflon discs with the materials as well as empty discs serving as control were placed in wells of 12-well plate. After incubation times of 1, 6, 20 and 24 hours, the teflon discs were removed from the wells and the number of viable cells was determined using trypan blue in Neubauer chamber. Results: In comparison to the control group, MTA Fillapex had significantly less viable cells for all incubation periods (p≤0.05), while Endosequence BC sealer had significantly less viable cells after 6, 20, and 24 hours of incubation (p≤0.05). MTA Fillapex exhibited ignificantly less viable cells in comparison to Endosequence BC sealer after the first hour and after 20 hours of incubation (p≤0.05), while for the other incubation periods there were no significant differences (p≥0.05). Conclusion: MTA Fillapex and Endosequence BC sealer were both cytotoxic in cultures of mouse L929 fibroblasts.https://hrcak.srce.hr/file/227450Root Canal Filling MaterialsAluminium CompoundsCalcium CompoundsToxicity Tests |
| spellingShingle | Anja Baraba Sonja Pezelj-Ribarić Marija Roguljić Ivana Miletić Cytotoxicity of Two Bioactive Root Canal Sealers Acta Stomatologica Croatica Root Canal Filling Materials Aluminium Compounds Calcium Compounds Toxicity Tests |
| title | Cytotoxicity of Two Bioactive Root Canal Sealers |
| title_full | Cytotoxicity of Two Bioactive Root Canal Sealers |
| title_fullStr | Cytotoxicity of Two Bioactive Root Canal Sealers |
| title_full_unstemmed | Cytotoxicity of Two Bioactive Root Canal Sealers |
| title_short | Cytotoxicity of Two Bioactive Root Canal Sealers |
| title_sort | cytotoxicity of two bioactive root canal sealers |
| topic | Root Canal Filling Materials Aluminium Compounds Calcium Compounds Toxicity Tests |
| url | https://hrcak.srce.hr/file/227450 |
| work_keys_str_mv | AT anjabaraba cytotoxicityoftwobioactiverootcanalsealers AT sonjapezeljribaric cytotoxicityoftwobioactiverootcanalsealers AT marijaroguljic cytotoxicityoftwobioactiverootcanalsealers AT ivanamiletic cytotoxicityoftwobioactiverootcanalsealers |