Molecular combing and its application in clinical settings
Abstract Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cove...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Molecular Cytogenetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13039-022-00628-8 |
| _version_ | 1811219409787682816 |
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| author | Yiping Wang Kishore Ramesh Kumar Thomas Liehr |
| author_facet | Yiping Wang Kishore Ramesh Kumar Thomas Liehr |
| author_sort | Yiping Wang |
| collection | DOAJ |
| description | Abstract Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cover slips. Many DNA molecules can be stretched at the same time in parallel and neatly arranged side-by-side, making the approach convenient for statistical analysis. Accordingly, DNA replication and transcription can be studied at the single molecule level. In this paper, the principle, experimental methods, important applications, advantages and shortcuts of MCT in medical field are presented and discussed. |
| first_indexed | 2024-04-12T07:25:16Z |
| format | Article |
| id | doaj.art-cc489f8d50004f2eb37d916c410985ea |
| institution | Directory Open Access Journal |
| issn | 1755-8166 |
| language | English |
| last_indexed | 2024-04-12T07:25:16Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cytogenetics |
| spelling | doaj.art-cc489f8d50004f2eb37d916c410985ea2022-12-22T03:42:12ZengBMCMolecular Cytogenetics1755-81662022-11-011511710.1186/s13039-022-00628-8Molecular combing and its application in clinical settingsYiping Wang0Kishore Ramesh Kumar1Thomas Liehr2Institute of Human Genetics, Jena University Hospital, Friedrich Schiller UniversityInstitute of Human Genetics, Jena University Hospital, Friedrich Schiller UniversityInstitute of Human Genetics, Jena University Hospital, Friedrich Schiller UniversityAbstract Molecular combing technology (MCT) is an effective means for stretching DNA molecules and making them thus accessible for in situ studies. MCT uses the force exerted in the process of liquid flow via surface tension to stretch DNA molecules and spread them on solid surfaces, i.e. glass cover slips. Many DNA molecules can be stretched at the same time in parallel and neatly arranged side-by-side, making the approach convenient for statistical analysis. Accordingly, DNA replication and transcription can be studied at the single molecule level. In this paper, the principle, experimental methods, important applications, advantages and shortcuts of MCT in medical field are presented and discussed.https://doi.org/10.1186/s13039-022-00628-8Molecular combingFluorescence in situ hybridization (FISH)GenomicsConstitutional genetic diseasesAcquired genetic diseases |
| spellingShingle | Yiping Wang Kishore Ramesh Kumar Thomas Liehr Molecular combing and its application in clinical settings Molecular Cytogenetics Molecular combing Fluorescence in situ hybridization (FISH) Genomics Constitutional genetic diseases Acquired genetic diseases |
| title | Molecular combing and its application in clinical settings |
| title_full | Molecular combing and its application in clinical settings |
| title_fullStr | Molecular combing and its application in clinical settings |
| title_full_unstemmed | Molecular combing and its application in clinical settings |
| title_short | Molecular combing and its application in clinical settings |
| title_sort | molecular combing and its application in clinical settings |
| topic | Molecular combing Fluorescence in situ hybridization (FISH) Genomics Constitutional genetic diseases Acquired genetic diseases |
| url | https://doi.org/10.1186/s13039-022-00628-8 |
| work_keys_str_mv | AT yipingwang molecularcombinganditsapplicationinclinicalsettings AT kishorerameshkumar molecularcombinganditsapplicationinclinicalsettings AT thomasliehr molecularcombinganditsapplicationinclinicalsettings |