Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood
As the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range of biological functions and has high flow characteristics. Small RNA sequencing of pig umbilical venous blood (UVB) and umbilical arterial...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1563 |
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| author | Mailin Gan Lin Liu Shunhua Zhang Zongyi Guo Ya Tan Jia Luo Qiong Yang Hongmei Pan Xuewei Li Jinyong Wang Linyuan Shen Li Zhu |
| author_facet | Mailin Gan Lin Liu Shunhua Zhang Zongyi Guo Ya Tan Jia Luo Qiong Yang Hongmei Pan Xuewei Li Jinyong Wang Linyuan Shen Li Zhu |
| author_sort | Mailin Gan |
| collection | DOAJ |
| description | As the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range of biological functions and has high flow characteristics. Small RNA sequencing of pig umbilical venous blood (UVB) and umbilical arterial blood (UAB) revealed that a total of 302 miRNAs were identified, and 106 and 22 miRNAs were specifically expressed in the UVB and UAB, respectively. Using the two methods of differential expression multiple and differential expression percentage, it is found that only 35% of the highly expressed miRNAs in the UVB by the two analysis modes overlap, but 56.25% of the enriched signal pathways are the same. Only 20% of the highly expressed miRNAs in the UAB overlap, but 62.07% of the signal pathways are the same. Further analysis revealed that miR-423 can be used as a characteristic miRNA of UVB and has the potential to treat muscle-related diseases. miR-122-5p can be used as a characteristic miRNA of UAB and may help to improve liver- and brain-related diseases. In summary, these results enrich understanding of miRNA in mother–fetal communication and provide a reference for the development and application of porcine cord blood products. |
| first_indexed | 2024-03-10T10:59:49Z |
| format | Article |
| id | doaj.art-cc493b621aef4f2199b2d2babb5c62ea |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:59:49Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cc493b621aef4f2199b2d2babb5c62ea2023-11-21T21:37:45ZengMDPI AGAnimals2076-26152021-05-01116156310.3390/ani11061563Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial BloodMailin Gan0Lin Liu1Shunhua Zhang2Zongyi Guo3Ya Tan4Jia Luo5Qiong Yang6Hongmei Pan7Xuewei Li8Jinyong Wang9Linyuan Shen10Li Zhu11College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaChongqing Academy of Animal Science, Rongchang, Chongqing 402460, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Southwest University, Chongqing 400715, ChinaDepartment of Animal Husbandry and Veterinary Medicine, Chengdu Agricultural College, Chengdu 611100, ChinaChongqing Academy of Animal Science, Rongchang, Chongqing 402460, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaChongqing Academy of Animal Science, Rongchang, Chongqing 402460, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaAs the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range of biological functions and has high flow characteristics. Small RNA sequencing of pig umbilical venous blood (UVB) and umbilical arterial blood (UAB) revealed that a total of 302 miRNAs were identified, and 106 and 22 miRNAs were specifically expressed in the UVB and UAB, respectively. Using the two methods of differential expression multiple and differential expression percentage, it is found that only 35% of the highly expressed miRNAs in the UVB by the two analysis modes overlap, but 56.25% of the enriched signal pathways are the same. Only 20% of the highly expressed miRNAs in the UAB overlap, but 62.07% of the signal pathways are the same. Further analysis revealed that miR-423 can be used as a characteristic miRNA of UVB and has the potential to treat muscle-related diseases. miR-122-5p can be used as a characteristic miRNA of UAB and may help to improve liver- and brain-related diseases. In summary, these results enrich understanding of miRNA in mother–fetal communication and provide a reference for the development and application of porcine cord blood products.https://www.mdpi.com/2076-2615/11/6/1563pigumbilical cord bloodmiRNAsdiseases |
| spellingShingle | Mailin Gan Lin Liu Shunhua Zhang Zongyi Guo Ya Tan Jia Luo Qiong Yang Hongmei Pan Xuewei Li Jinyong Wang Linyuan Shen Li Zhu Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood Animals pig umbilical cord blood miRNAs diseases |
| title | Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood |
| title_full | Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood |
| title_fullStr | Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood |
| title_full_unstemmed | Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood |
| title_short | Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood |
| title_sort | expression characteristics of microrna in pig umbilical venous blood and umbilical arterial blood |
| topic | pig umbilical cord blood miRNAs diseases |
| url | https://www.mdpi.com/2076-2615/11/6/1563 |
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