Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation
Ultrasound can influence biological systems through several distinct acoustic mechanisms that can be manipulated by varying reaction conditions and acoustic exposure parameters. We recently reported a new ultrasound-based fabrication technology that exploits the ability of ultrasound to generate loc...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/8/2907 |
| _version_ | 1797569892020387840 |
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| author | Emma G. Norris Diane Dalecki Denise C. Hocking |
| author_facet | Emma G. Norris Diane Dalecki Denise C. Hocking |
| author_sort | Emma G. Norris |
| collection | DOAJ |
| description | Ultrasound can influence biological systems through several distinct acoustic mechanisms that can be manipulated by varying reaction conditions and acoustic exposure parameters. We recently reported a new ultrasound-based fabrication technology that exploits the ability of ultrasound to generate localized mechanical forces and thermal effects to control collagen fiber microstructure non-invasively. Exposing solutions of type I collagen to ultrasound during the period of microfibril assembly produced changes in collagen fiber structure and alignment, and increased the biological activity of the resultant collagen hydrogels. In the extracellular matrix, interactions between fibronectin and collagen fibrils influence the biological activity of both proteins. Thus, in the present study, we examined how addition of fibronectin to collagen solutions prior to ultrasound exposure affects protein organization and the biological activity of the composite hydrogels. Results indicate that ultrasound can alter the distribution of fibronectin within 3D hydrogels via thermal and non-thermal mechanisms to produce composite hydrogels that support accelerated microtissue formation. The use of acoustic energy to drive changes in protein conformation to functionalize biomaterials has much potential as a unique, non-invasive technology for tissue engineering and regenerative medicine. |
| first_indexed | 2024-03-10T20:17:45Z |
| format | Article |
| id | doaj.art-cc4a166293a8410c9fdfb294e371a895 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:17:45Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-cc4a166293a8410c9fdfb294e371a8952023-11-19T22:26:33ZengMDPI AGApplied Sciences2076-34172020-04-01108290710.3390/app10082907Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue FormationEmma G. Norris0Diane Dalecki1Denise C. Hocking2Department of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USADepartment of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USADepartment of Pharmacology and Physiology, University of Rochester, Rochester, NY 14642, USAUltrasound can influence biological systems through several distinct acoustic mechanisms that can be manipulated by varying reaction conditions and acoustic exposure parameters. We recently reported a new ultrasound-based fabrication technology that exploits the ability of ultrasound to generate localized mechanical forces and thermal effects to control collagen fiber microstructure non-invasively. Exposing solutions of type I collagen to ultrasound during the period of microfibril assembly produced changes in collagen fiber structure and alignment, and increased the biological activity of the resultant collagen hydrogels. In the extracellular matrix, interactions between fibronectin and collagen fibrils influence the biological activity of both proteins. Thus, in the present study, we examined how addition of fibronectin to collagen solutions prior to ultrasound exposure affects protein organization and the biological activity of the composite hydrogels. Results indicate that ultrasound can alter the distribution of fibronectin within 3D hydrogels via thermal and non-thermal mechanisms to produce composite hydrogels that support accelerated microtissue formation. The use of acoustic energy to drive changes in protein conformation to functionalize biomaterials has much potential as a unique, non-invasive technology for tissue engineering and regenerative medicine.https://www.mdpi.com/2076-3417/10/8/2907ultrasoundcollagenfibronectinhydrogeltissue engineeringacoustics |
| spellingShingle | Emma G. Norris Diane Dalecki Denise C. Hocking Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation Applied Sciences ultrasound collagen fibronectin hydrogel tissue engineering acoustics |
| title | Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation |
| title_full | Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation |
| title_fullStr | Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation |
| title_full_unstemmed | Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation |
| title_short | Acoustic Fabrication of Collagen–Fibronectin Composite Gels Accelerates Microtissue Formation |
| title_sort | acoustic fabrication of collagen fibronectin composite gels accelerates microtissue formation |
| topic | ultrasound collagen fibronectin hydrogel tissue engineering acoustics |
| url | https://www.mdpi.com/2076-3417/10/8/2907 |
| work_keys_str_mv | AT emmagnorris acousticfabricationofcollagenfibronectincompositegelsacceleratesmicrotissueformation AT dianedalecki acousticfabricationofcollagenfibronectincompositegelsacceleratesmicrotissueformation AT denisechocking acousticfabricationofcollagenfibronectincompositegelsacceleratesmicrotissueformation |