Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy
Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/8/1026 |
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| author | Gianluca Morroni Simona Fioriti Federica Salari Andrea Brenciani Lucia Brescini Marina Mingoia Eleonora Giovanetti Antonella Pocognoli Andrea Giacometti Elisa Molinelli Annamaria Offidani Oriana Simonetti Oscar Cirioni |
| author_facet | Gianluca Morroni Simona Fioriti Federica Salari Andrea Brenciani Lucia Brescini Marina Mingoia Eleonora Giovanetti Antonella Pocognoli Andrea Giacometti Elisa Molinelli Annamaria Offidani Oriana Simonetti Oscar Cirioni |
| author_sort | Gianluca Morroni |
| collection | DOAJ |
| description | Background: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCC<i>mec</i>I, ST22/CC22-t18014-SCC<i>mec</i>IV, and ST22/CC22-t022-SCC<i>mec</i>IV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCC<i>mec</i>I strains and all the other PBPs for ST22-t18014-SCC<i>mec</i>IV and ST22-t022-SCC<i>mec</i>IV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections. |
| first_indexed | 2024-03-10T09:03:41Z |
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| id | doaj.art-cc4ad333bc2f4da680d982141988a7f2 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T09:03:41Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-cc4ad333bc2f4da680d982141988a7f22023-11-22T06:34:53ZengMDPI AGAntibiotics2079-63822021-08-01108102610.3390/antibiotics10081026Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central ItalyGianluca Morroni0Simona Fioriti1Federica Salari2Andrea Brenciani3Lucia Brescini4Marina Mingoia5Eleonora Giovanetti6Antonella Pocognoli7Andrea Giacometti8Elisa Molinelli9Annamaria Offidani10Oriana Simonetti11Oscar Cirioni12Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Life and Environmental Sciences, Polytechnic University of Marche, 60126 Ancona, ItalyClinical Microbiology Laboratory, “Ospedali Riuniti”, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyDermatological Clinic, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, ItalyDermatological Clinic, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, ItalyDermatological Clinic, Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, ItalyDepartment of Biomedical Sciences and Public Health, Polytechnic University of Marche, 60126 Ancona, ItalyBackground: Ceftaroline represents a novel fifth-generation cephalosporin to treat infections caused by methicillin-resistant Staphylococcus aureus (MRSA). Methods: Ceftaroline susceptibility of 239 MRSA isolates was assessed by disk diffusion and a MIC test strip following both EUCAST and CLSI guidelines. Non-susceptible isolates were epidemiologically characterized by pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing, and further investigated by PCR and whole genome sequencing to detect penicillin-binding protein (PBP) mutations as well as antibiotic resistance and virulence genes. Results: Fourteen isolates out of two hundred and thirty-nine (5.8%) were non-susceptible to ceftaroline (MIC > 1 mg/L), with differences between the EUCAST and CLSI interpretations. The characterized isolates belonged to seven different pulsotypes and three different clones (ST228/CC5-t041-SCC<i>mec</i>I, ST22/CC22-t18014-SCC<i>mec</i>IV, and ST22/CC22-t022-SCC<i>mec</i>IV), confirming a clonal diffusion of ceftaroline non-susceptible strains. Mutations in PBPs involved PBP2a for ST228-t041-SCC<i>mec</i>I strains and all the other PBPs for ST22-t18014-SCC<i>mec</i>IV and ST22-t022-SCC<i>mec</i>IV clones. All isolates harbored antibiotic resistance and virulence genes with a clonal distribution. Conclusion: Our study demonstrated that ceftaroline non-susceptibile isolates belonged not only to ST228 strains (the most widespread clone in Italy) but also to ST22, confirming the increasing role of these clones in hospital infections.https://www.mdpi.com/2079-6382/10/8/1026ceftarolineMRSApenicillin-binding proteinsSCC<i>mec</i> |
| spellingShingle | Gianluca Morroni Simona Fioriti Federica Salari Andrea Brenciani Lucia Brescini Marina Mingoia Eleonora Giovanetti Antonella Pocognoli Andrea Giacometti Elisa Molinelli Annamaria Offidani Oriana Simonetti Oscar Cirioni Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy Antibiotics ceftaroline MRSA penicillin-binding proteins SCC<i>mec</i> |
| title | Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy |
| title_full | Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy |
| title_fullStr | Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy |
| title_full_unstemmed | Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy |
| title_short | Characterization and Clonal Diffusion of Ceftaroline Non-Susceptible MRSA in Two Hospitals in Central Italy |
| title_sort | characterization and clonal diffusion of ceftaroline non susceptible mrsa in two hospitals in central italy |
| topic | ceftaroline MRSA penicillin-binding proteins SCC<i>mec</i> |
| url | https://www.mdpi.com/2079-6382/10/8/1026 |
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