Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i>
The current COVID-19 pandemic very dramatically shows that the world lacks preparedness for novel viral diseases. In addition to newly emerging viruses, many known pathogenic viruses such as influenza are constantly evolving, leading to frequent outbreaks with severe diseases and deaths. Hence, infe...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/8/1093 |
| _version_ | 1797434318294876160 |
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| author | Valentina Ruocco Richard Strasser |
| author_facet | Valentina Ruocco Richard Strasser |
| author_sort | Valentina Ruocco |
| collection | DOAJ |
| description | The current COVID-19 pandemic very dramatically shows that the world lacks preparedness for novel viral diseases. In addition to newly emerging viruses, many known pathogenic viruses such as influenza are constantly evolving, leading to frequent outbreaks with severe diseases and deaths. Hence, infectious viruses are a recurrent burden to our daily life, and powerful strategies to stop the spread of human pathogens and disease progression are of utmost importance. Transient plant-based protein expression is a technology that allows fast and highly flexible manufacturing of recombinant viral proteins and, thus, can contribute to infectious disease detection and prevention. This review highlights recent progress in the transient production of viral glycoproteins in <i>N. benthamiana</i> with a focus on SARS-CoV-2-derived viral antigens. |
| first_indexed | 2024-03-09T10:30:28Z |
| format | Article |
| id | doaj.art-cc4e25f58a034c9e9e707497304e4b6c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T10:30:28Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-cc4e25f58a034c9e9e707497304e4b6c2023-12-01T21:19:50ZengMDPI AGPlants2223-77472022-04-01118109310.3390/plants11081093Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i>Valentina Ruocco0Richard Strasser1Department of Applied Genetics and Cell Biology, Institute of Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, AustriaDepartment of Applied Genetics and Cell Biology, Institute of Plant Biotechnology and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, A-1190 Vienna, AustriaThe current COVID-19 pandemic very dramatically shows that the world lacks preparedness for novel viral diseases. In addition to newly emerging viruses, many known pathogenic viruses such as influenza are constantly evolving, leading to frequent outbreaks with severe diseases and deaths. Hence, infectious viruses are a recurrent burden to our daily life, and powerful strategies to stop the spread of human pathogens and disease progression are of utmost importance. Transient plant-based protein expression is a technology that allows fast and highly flexible manufacturing of recombinant viral proteins and, thus, can contribute to infectious disease detection and prevention. This review highlights recent progress in the transient production of viral glycoproteins in <i>N. benthamiana</i> with a focus on SARS-CoV-2-derived viral antigens.https://www.mdpi.com/2223-7747/11/8/1093COVID-19glycoproteinglycosylationmolecular farmingplant expression systemvirus |
| spellingShingle | Valentina Ruocco Richard Strasser Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> Plants COVID-19 glycoprotein glycosylation molecular farming plant expression system virus |
| title | Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> |
| title_full | Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> |
| title_fullStr | Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> |
| title_full_unstemmed | Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> |
| title_short | Transient Expression of Glycosylated SARS-CoV-2 Antigens in <i>Nicotiana benthamiana</i> |
| title_sort | transient expression of glycosylated sars cov 2 antigens in i nicotiana benthamiana i |
| topic | COVID-19 glycoprotein glycosylation molecular farming plant expression system virus |
| url | https://www.mdpi.com/2223-7747/11/8/1093 |
| work_keys_str_mv | AT valentinaruocco transientexpressionofglycosylatedsarscov2antigensininicotianabenthamianai AT richardstrasser transientexpressionofglycosylatedsarscov2antigensininicotianabenthamianai |