Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens
Summary: Sclerotinia sclerotiorum and Botrytis cinerea are necrotrophic plant-pathogenic fungi, causing substantial economic losses on many crops. So far, resistant cultivars against these pathogens are unavailable in most crops. Here, we show that the serine protease CmSp1 of Coniothyrium minitans,...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723013025 |
| _version_ | 1797448147082936320 |
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| author | Yongchun Wang Han Yu Yuping Xu Mingde Wu Jing Zhang Kenichi Tsuda Shengyi Liu Daohong Jiang Weidong Chen Yangdou Wei Guoqing Li Long Yang |
| author_facet | Yongchun Wang Han Yu Yuping Xu Mingde Wu Jing Zhang Kenichi Tsuda Shengyi Liu Daohong Jiang Weidong Chen Yangdou Wei Guoqing Li Long Yang |
| author_sort | Yongchun Wang |
| collection | DOAJ |
| description | Summary: Sclerotinia sclerotiorum and Botrytis cinerea are necrotrophic plant-pathogenic fungi, causing substantial economic losses on many crops. So far, resistant cultivars against these pathogens are unavailable in most crops. Here, we show that the serine protease CmSp1 of Coniothyrium minitans, a well-characterized mycoparasite of S. sclerotiorum, contributed to suppressing the petal-mediated infection by S. sclerotiorum in rapeseed. Application of recombinant CmSp1 proteins facilitates the bulk degradation of S. sclerotiorum proteins and inhibits spore germination and hyphal growth of S. sclerotiorum and B. cinerea, thereby preventing the development of both diseases. Stable transgenic rapeseed plants with tissue-specific expression of CmSp1 in flower petals inhibit the petal-mediated infection by both S. sclerotiorum and B. cinerea, and resulting transgenic plants have no adverse effect on other agronomic traits. Thus, our findings provide a novel mechanism by which a mycoparasite inhibits fungal pathogens and an environmentally friendly disease management strategy. |
| first_indexed | 2024-03-09T14:06:16Z |
| format | Article |
| id | doaj.art-cc4efc45e926438985e214105c61edfb |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-09T14:06:16Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-cc4efc45e926438985e214105c61edfb2023-11-30T05:06:40ZengElsevierCell Reports2211-12472023-11-014211113290Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogensYongchun Wang0Han Yu1Yuping Xu2Mingde Wu3Jing Zhang4Kenichi Tsuda5Shengyi Liu6Daohong Jiang7Weidong Chen8Yangdou Wei9Guoqing Li10Long Yang11State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, ChinaKey Laboratory of Biology and Genetic Improvement of Oil Crops, Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; Hubei Hongshan Laboratory, Wuhan, ChinaU.S. Department of Agriculture, Agricultural Research Service, Washington State University, Pullman, WA, USADepartment of Biology, University of Saskatchewan, Saskatoon, SK, CanadaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China; Corresponding authorSummary: Sclerotinia sclerotiorum and Botrytis cinerea are necrotrophic plant-pathogenic fungi, causing substantial economic losses on many crops. So far, resistant cultivars against these pathogens are unavailable in most crops. Here, we show that the serine protease CmSp1 of Coniothyrium minitans, a well-characterized mycoparasite of S. sclerotiorum, contributed to suppressing the petal-mediated infection by S. sclerotiorum in rapeseed. Application of recombinant CmSp1 proteins facilitates the bulk degradation of S. sclerotiorum proteins and inhibits spore germination and hyphal growth of S. sclerotiorum and B. cinerea, thereby preventing the development of both diseases. Stable transgenic rapeseed plants with tissue-specific expression of CmSp1 in flower petals inhibit the petal-mediated infection by both S. sclerotiorum and B. cinerea, and resulting transgenic plants have no adverse effect on other agronomic traits. Thus, our findings provide a novel mechanism by which a mycoparasite inhibits fungal pathogens and an environmentally friendly disease management strategy.http://www.sciencedirect.com/science/article/pii/S2211124723013025CP: Plants |
| spellingShingle | Yongchun Wang Han Yu Yuping Xu Mingde Wu Jing Zhang Kenichi Tsuda Shengyi Liu Daohong Jiang Weidong Chen Yangdou Wei Guoqing Li Long Yang Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens Cell Reports CP: Plants |
| title | Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens |
| title_full | Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens |
| title_fullStr | Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens |
| title_full_unstemmed | Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens |
| title_short | Expression of a mycoparasite protease in plant petals suppresses the petal-mediated infection by necrotrophic pathogens |
| title_sort | expression of a mycoparasite protease in plant petals suppresses the petal mediated infection by necrotrophic pathogens |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723013025 |
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