Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences
Background: The World Health Organization declared the coronavirus disease 2019 (COVID-19) a global pandemic on 11 March 2020. Identifying the infected people and isolating them was the only measure that was available to control the viral spread, as there were no standardized treatment interventions...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2023.1122715/full |
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| author | Sanchita Roy Pradhan M. H. Yashavarddhan Ashish Gupta Pramod Kumar Anuj Kumar Nazneen Arif Usha Agrawal R. Suresh Kumar Shalini Singh |
| author_facet | Sanchita Roy Pradhan M. H. Yashavarddhan Ashish Gupta Pramod Kumar Anuj Kumar Nazneen Arif Usha Agrawal R. Suresh Kumar Shalini Singh |
| author_sort | Sanchita Roy Pradhan |
| collection | DOAJ |
| description | Background: The World Health Organization declared the coronavirus disease 2019 (COVID-19) a global pandemic on 11 March 2020. Identifying the infected people and isolating them was the only measure that was available to control the viral spread, as there were no standardized treatment interventions available. Various public health measures, including vaccination, have been implemented to control the spread of the virus worldwide. India, being a densely populated country, required laboratories in different zones of the country with the capacity to test a large number of samples and report test results at the earliest. The Indian Council of Medical Research (ICMR) took the lead role in developing policies, generating advisories, formulating guidelines, and establishing and approving testing centers for COVID-19 testing. With advisories of ICMR, the National Institute of Cancer Prevention and Research (NICPR) established a high-throughput viral diagnostic laboratory (HTVDL) for RT-PCR-based diagnosis of SARS-CoV-2 in April 2020. HTVDL was established during the first lockdown to serve the nation in developing and adopting rapid testing procedures and to expand the testing capacity using “Real-Time PCR.” The HTVDL provided its testing support to the national capital territory of Delhi and western Uttar Pradesh, with a testing capacity of 6000 tests per day. The experience of establishing a high-throughput laboratory with all standard operating procedures against varied challenges in a developing country such as India is explained in the current manuscript which will be useful globally to enhance the knowledge on establishing an HTVDL in pandemic or non-pandemic times. |
| first_indexed | 2024-04-09T17:30:49Z |
| format | Article |
| id | doaj.art-cc4f48be83f54e25b61baf3745863ef4 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-04-09T17:30:49Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-cc4f48be83f54e25b61baf3745863ef42023-04-18T06:14:48ZengFrontiers Media S.A.Frontiers in Public Health2296-25652023-04-011110.3389/fpubh.2023.11227151122715Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiencesSanchita Roy Pradhan0M. H. Yashavarddhan1Ashish Gupta2Pramod Kumar3Anuj Kumar4Nazneen Arif5Usha Agrawal6R. Suresh Kumar7Shalini Singh8High-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaDepartment of Histopathology and Cytology, National Institute of Pathology, Safdarjung Hospital, New Delhi, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaHigh-Throughput Viral Diagnostic Laboratory (HTVDL), ICMR - National Institute of Cancer Prevention and Research (NICPR), Ministry of Health and Family Welfare, Government of India, Noida, Uttar Pradesh, IndiaBackground: The World Health Organization declared the coronavirus disease 2019 (COVID-19) a global pandemic on 11 March 2020. Identifying the infected people and isolating them was the only measure that was available to control the viral spread, as there were no standardized treatment interventions available. Various public health measures, including vaccination, have been implemented to control the spread of the virus worldwide. India, being a densely populated country, required laboratories in different zones of the country with the capacity to test a large number of samples and report test results at the earliest. The Indian Council of Medical Research (ICMR) took the lead role in developing policies, generating advisories, formulating guidelines, and establishing and approving testing centers for COVID-19 testing. With advisories of ICMR, the National Institute of Cancer Prevention and Research (NICPR) established a high-throughput viral diagnostic laboratory (HTVDL) for RT-PCR-based diagnosis of SARS-CoV-2 in April 2020. HTVDL was established during the first lockdown to serve the nation in developing and adopting rapid testing procedures and to expand the testing capacity using “Real-Time PCR.” The HTVDL provided its testing support to the national capital territory of Delhi and western Uttar Pradesh, with a testing capacity of 6000 tests per day. The experience of establishing a high-throughput laboratory with all standard operating procedures against varied challenges in a developing country such as India is explained in the current manuscript which will be useful globally to enhance the knowledge on establishing an HTVDL in pandemic or non-pandemic times.https://www.frontiersin.org/articles/10.3389/fpubh.2023.1122715/fullSARS CoV-2coronavirusCOVID-19viral diagnosisRT-PCR |
| spellingShingle | Sanchita Roy Pradhan M. H. Yashavarddhan Ashish Gupta Pramod Kumar Anuj Kumar Nazneen Arif Usha Agrawal R. Suresh Kumar Shalini Singh Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences Frontiers in Public Health SARS CoV-2 coronavirus COVID-19 viral diagnosis RT-PCR |
| title | Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences |
| title_full | Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences |
| title_fullStr | Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences |
| title_full_unstemmed | Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences |
| title_short | Insights from establishing a high throughput viral diagnostic laboratory for SARS-CoV-2 RT-PCR testing facility: challenges and experiences |
| title_sort | insights from establishing a high throughput viral diagnostic laboratory for sars cov 2 rt pcr testing facility challenges and experiences |
| topic | SARS CoV-2 coronavirus COVID-19 viral diagnosis RT-PCR |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2023.1122715/full |
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