Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation
Consumption of <i>Bifidobacterium breve</i> MCC1274 has been shown to improve memory and prevent brain atrophy in populations with mild cognitive impairment (MCI). Preclinical in vivo studies using Alzheimer’s disease (AD) models indicate that this probiotic protects against brain inflam...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/791 |
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| author | François Bernier Tatsuya Kuhara Jinzhong Xiao |
| author_facet | François Bernier Tatsuya Kuhara Jinzhong Xiao |
| author_sort | François Bernier |
| collection | DOAJ |
| description | Consumption of <i>Bifidobacterium breve</i> MCC1274 has been shown to improve memory and prevent brain atrophy in populations with mild cognitive impairment (MCI). Preclinical in vivo studies using Alzheimer’s disease (AD) models indicate that this probiotic protects against brain inflammation. There is growing evidence that lipid droplets are associated with brain inflammation, and lipid-associated proteins called perilipins could play an important role in neurodegenerative diseases such as dementia. In this study, we found that <i>B. breve</i> MCC1274 cell extracts significantly decreased the expression of perilipin 4 (<i>PLIN4</i>), which encodes a lipid droplet docking protein whose expression is known to be increased during inflammation in SH-SY5Y cells. Niacin, an MCC1274 cell extract component, increased <i>PLIN4</i> expression by itself. Moreover, MCC1274 cell extracts and niacin blocked the PLIN4 induction caused by oxidative stress in SH-SY5Y cells, reduced lipid droplet formation, and prevented IL-6 cytokine production. These results offer a possible explanation for the effect of this strain on brain inflammation. |
| first_indexed | 2024-03-11T06:09:41Z |
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| id | doaj.art-cc50712d02094ee88c1afc0a8be99f89 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T06:09:41Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-cc50712d02094ee88c1afc0a8be99f892023-11-17T12:46:08ZengMDPI AGMicroorganisms2076-26072023-03-0111379110.3390/microorganisms11030791Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene RegulationFrançois Bernier0Tatsuya Kuhara1Jinzhong Xiao2Next Generation Science Institute, R&D Division, Morinaga Milk Industry, Co., Ltd., Zama 252-8583, JapanNext Generation Science Institute, R&D Division, Morinaga Milk Industry, Co., Ltd., Zama 252-8583, JapanNext Generation Science Institute, R&D Division, Morinaga Milk Industry, Co., Ltd., Zama 252-8583, JapanConsumption of <i>Bifidobacterium breve</i> MCC1274 has been shown to improve memory and prevent brain atrophy in populations with mild cognitive impairment (MCI). Preclinical in vivo studies using Alzheimer’s disease (AD) models indicate that this probiotic protects against brain inflammation. There is growing evidence that lipid droplets are associated with brain inflammation, and lipid-associated proteins called perilipins could play an important role in neurodegenerative diseases such as dementia. In this study, we found that <i>B. breve</i> MCC1274 cell extracts significantly decreased the expression of perilipin 4 (<i>PLIN4</i>), which encodes a lipid droplet docking protein whose expression is known to be increased during inflammation in SH-SY5Y cells. Niacin, an MCC1274 cell extract component, increased <i>PLIN4</i> expression by itself. Moreover, MCC1274 cell extracts and niacin blocked the PLIN4 induction caused by oxidative stress in SH-SY5Y cells, reduced lipid droplet formation, and prevented IL-6 cytokine production. These results offer a possible explanation for the effect of this strain on brain inflammation.https://www.mdpi.com/2076-2607/11/3/791<i>Bifidobacterium breve</i>PLIN4lipid dropletsniacinoxidative stressinflammation |
| spellingShingle | François Bernier Tatsuya Kuhara Jinzhong Xiao Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation Microorganisms <i>Bifidobacterium breve</i> PLIN4 lipid droplets niacin oxidative stress inflammation |
| title | Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation |
| title_full | Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation |
| title_fullStr | Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation |
| title_full_unstemmed | Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation |
| title_short | Probiotic <i>Bifidobacterium breve</i> MCC1274 Protects against Oxidative Stress and Neuronal Lipid Droplet Formation via PLIN4 Gene Regulation |
| title_sort | probiotic i bifidobacterium breve i mcc1274 protects against oxidative stress and neuronal lipid droplet formation via plin4 gene regulation |
| topic | <i>Bifidobacterium breve</i> PLIN4 lipid droplets niacin oxidative stress inflammation |
| url | https://www.mdpi.com/2076-2607/11/3/791 |
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