Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area
<i>Helicobacter pylori</i> (<i>H. pylori</i>) related chronic gastritis is a well-known major etiological factor for gastric cancer development. However, <i>H. pylori</i>-negative gastritis (HpN) is not well described. We aimed to examine gastric mucosal microbiot...
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MDPI AG
2019-04-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/11/4/504 |
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| author | Boldbaatar Gantuya Hashem B. El-Serag Takashi Matsumoto Nadim J. Ajami Khasag Oyuntsetseg Dashdorj Azzaya Tomohisa Uchida Yoshio Yamaoka |
| author_facet | Boldbaatar Gantuya Hashem B. El-Serag Takashi Matsumoto Nadim J. Ajami Khasag Oyuntsetseg Dashdorj Azzaya Tomohisa Uchida Yoshio Yamaoka |
| author_sort | Boldbaatar Gantuya |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> (<i>H. pylori</i>) related chronic gastritis is a well-known major etiological factor for gastric cancer development. However, <i>H. pylori</i>-negative gastritis (HpN) is not well described. We aimed to examine gastric mucosal microbiota in HpN compared to <i>H. pylori</i>-positive gastritis (HpP) and <i>H. pylori</i>-negative non-gastritis group (control). Here, we studied 11 subjects with HpN, 40 with HpP and 24 controls. We performed endoscopy with six gastric biopsies. Comparison groups were defined based on strict histological criteria for the disease and <i>H. pylori</i> diagnosis. We used 16S rRNA gene amplicon sequencing to profile the gastric microbiota according to comparison groups. These results demonstrate that the HpP group had significantly lower bacterial richness by the operational taxonomic unit (OTU) counts, and Shannon and Simpson indices as compared to HpN or controls. The linear discriminant analysis effect size analysis showed the enrichment of Firmicutes, Fusobacteria, Bacteroidetes and Actinobacteria at phylum level in the HpN group. In the age-adjusted multivariate analysis, <i>Streptococcus</i> sp. and <i>Haemophilus parainfluenzae</i> were at a significantly increased risk for HpN (odds ratio 18.9 and 12.3, respectively) based on abundance. <i>Treponema</i> sp. was uniquely found in HpN based on occurrence. In this paper, we conclude that <i>Streptococcus</i> sp., <i>Haemophilus parainfluenzae</i> and <i>Treponema</i> sp. are candidate pathogenic bacterial species for HpN. These results if confirmed may have important clinical implications. |
| first_indexed | 2024-03-12T06:57:29Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T06:57:29Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-cc515d01345547d188da0ead27c8bceb2023-09-02T23:59:24ZengMDPI AGCancers2072-66942019-04-0111450410.3390/cancers11040504cancers11040504Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer AreaBoldbaatar Gantuya0Hashem B. El-Serag1Takashi Matsumoto2Nadim J. Ajami3Khasag Oyuntsetseg4Dashdorj Azzaya5Tomohisa Uchida6Yoshio Yamaoka7Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita 879-5593, JapanDepartment of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, 7200 Cambridge Street, Houston, TX 77030, USADepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita 879-5593, JapanAlkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USADepartment of Internal Medicine, Gastroenterology Unit, Mongolian National University of Medical Sciences, Zorig Street, Ulaanbaatar-14210, MongoliaDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita 879-5593, JapanDepartment of Molecular Pathology, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita 879-5593, JapanDepartment of Environmental and Preventive Medicine, Oita University Faculty of Medicine, 1-1 Idaigaoka, Hasama-machi, Yufu-City, Oita 879-5593, Japan<i>Helicobacter pylori</i> (<i>H. pylori</i>) related chronic gastritis is a well-known major etiological factor for gastric cancer development. However, <i>H. pylori</i>-negative gastritis (HpN) is not well described. We aimed to examine gastric mucosal microbiota in HpN compared to <i>H. pylori</i>-positive gastritis (HpP) and <i>H. pylori</i>-negative non-gastritis group (control). Here, we studied 11 subjects with HpN, 40 with HpP and 24 controls. We performed endoscopy with six gastric biopsies. Comparison groups were defined based on strict histological criteria for the disease and <i>H. pylori</i> diagnosis. We used 16S rRNA gene amplicon sequencing to profile the gastric microbiota according to comparison groups. These results demonstrate that the HpP group had significantly lower bacterial richness by the operational taxonomic unit (OTU) counts, and Shannon and Simpson indices as compared to HpN or controls. The linear discriminant analysis effect size analysis showed the enrichment of Firmicutes, Fusobacteria, Bacteroidetes and Actinobacteria at phylum level in the HpN group. In the age-adjusted multivariate analysis, <i>Streptococcus</i> sp. and <i>Haemophilus parainfluenzae</i> were at a significantly increased risk for HpN (odds ratio 18.9 and 12.3, respectively) based on abundance. <i>Treponema</i> sp. was uniquely found in HpN based on occurrence. In this paper, we conclude that <i>Streptococcus</i> sp., <i>Haemophilus parainfluenzae</i> and <i>Treponema</i> sp. are candidate pathogenic bacterial species for HpN. These results if confirmed may have important clinical implications.https://www.mdpi.com/2072-6694/11/4/504<i>Helicobacter pylori</i>non-<i>Helicobacter pylori</i>gastritismicrobiota16S rRNA |
| spellingShingle | Boldbaatar Gantuya Hashem B. El-Serag Takashi Matsumoto Nadim J. Ajami Khasag Oyuntsetseg Dashdorj Azzaya Tomohisa Uchida Yoshio Yamaoka Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area Cancers <i>Helicobacter pylori</i> non-<i>Helicobacter pylori</i> gastritis microbiota 16S rRNA |
| title | Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area |
| title_full | Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area |
| title_fullStr | Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area |
| title_full_unstemmed | Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area |
| title_short | Gastric Microbiota in <i>Helicobacter pylori</i>-Negative and -Positive Gastritis Among High Incidence of Gastric Cancer Area |
| title_sort | gastric microbiota in i helicobacter pylori i negative and positive gastritis among high incidence of gastric cancer area |
| topic | <i>Helicobacter pylori</i> non-<i>Helicobacter pylori</i> gastritis microbiota 16S rRNA |
| url | https://www.mdpi.com/2072-6694/11/4/504 |
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