EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis
Zhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Depa...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-04-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/egfp-egf1-conjugated-polylactic-co-glycolic-acid-nanoparticles-a-new-d-peer-reviewed-article-IJN |
| _version_ | 1819021424365404160 |
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| author | Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H |
| author_facet | Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H |
| author_sort | Wu Z |
| collection | DOAJ |
| description | Zhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China Background: EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticle (ENP) has a specific affinity to tissue factor (TF). The aim of this study was to investigate the target delivery of ENP to plaques and its uptake in a mouse model of atherosclerosis in vivo and in vitro. Materials and methods: Coumarin-6- and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)-loaded ENPs were synthesized using a double-emulsion method. Mouse vascular smooth muscle cells (VSMCs) were induced with MCP-1 to obtain an increased TF expression. Fluorescence microscopy and flow cytometry assay were performed to examine the uptake of coumarin-6-loaded ENPs in cellular models. An animal model of atherosclerosis was established with an ApoE (-/-) mouse fed with continuous high-fat diets for 14 weeks. DiR-loaded ENPs (DiR-ENPs) were injected via the caudal vein. The distribution of DiR-ENPs was examined through organ imaging and confocal laser scanning microscopy. Results: Results indicated TFs were highly expressed in the cellular model. The uptake of coumarin-6-loaded ENPs was significantly higher than that of common PLGA nanoparticles. Thickening of intima and lipid deposition in the aorta could be observed in atherosclerosis mouse models. Confocal laser scanning microscopy organ imaging showed ENPs accumulated in vessels with atherosclerotic plaques, which coincided with high expressions of TF. Conclusion: This study showed that EGFP-EGF1-conjugated PLGA nanoparticles could be effectively delivered to atherosclerotic plaques in vivo and taken up by VSMCs with high TF expressions in vitro. Thus, it could be a promising carrier for targeted therapy of atherosclerosis. Keywords: tissue factor, atherosclerosis, nanoparticle, target delivery, EGFP-EGF1 |
| first_indexed | 2024-12-21T04:06:53Z |
| format | Article |
| id | doaj.art-cc539813add1468a9d6b1b9bfd8a1e9f |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-21T04:06:53Z |
| publishDate | 2019-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-cc539813add1468a9d6b1b9bfd8a1e9f2022-12-21T19:16:33ZengDove Medical PressInternational Journal of Nanomedicine1178-20132019-04-01Volume 142609261845098EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosisWu ZChen CZhang BTang LShi WLiao DDi GDavis JRWang HZhilin Wu,1 Chen Chen,1 Bo Zhang,2 Liang Tang,2 Wei Shi,2 Danying Liao,2 Gaohong Di,1 Jacques RJ Davis,1 Hui Wang1 1Anesthesiology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China; 2Haematology Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, People’s Republic of China Background: EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) (PLGA) nanoparticle (ENP) has a specific affinity to tissue factor (TF). The aim of this study was to investigate the target delivery of ENP to plaques and its uptake in a mouse model of atherosclerosis in vivo and in vitro. Materials and methods: Coumarin-6- and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR)-loaded ENPs were synthesized using a double-emulsion method. Mouse vascular smooth muscle cells (VSMCs) were induced with MCP-1 to obtain an increased TF expression. Fluorescence microscopy and flow cytometry assay were performed to examine the uptake of coumarin-6-loaded ENPs in cellular models. An animal model of atherosclerosis was established with an ApoE (-/-) mouse fed with continuous high-fat diets for 14 weeks. DiR-loaded ENPs (DiR-ENPs) were injected via the caudal vein. The distribution of DiR-ENPs was examined through organ imaging and confocal laser scanning microscopy. Results: Results indicated TFs were highly expressed in the cellular model. The uptake of coumarin-6-loaded ENPs was significantly higher than that of common PLGA nanoparticles. Thickening of intima and lipid deposition in the aorta could be observed in atherosclerosis mouse models. Confocal laser scanning microscopy organ imaging showed ENPs accumulated in vessels with atherosclerotic plaques, which coincided with high expressions of TF. Conclusion: This study showed that EGFP-EGF1-conjugated PLGA nanoparticles could be effectively delivered to atherosclerotic plaques in vivo and taken up by VSMCs with high TF expressions in vitro. Thus, it could be a promising carrier for targeted therapy of atherosclerosis. Keywords: tissue factor, atherosclerosis, nanoparticle, target delivery, EGFP-EGF1https://www.dovepress.com/egfp-egf1-conjugated-polylactic-co-glycolic-acid-nanoparticles-a-new-d-peer-reviewed-article-IJNTissue factorAtherosclerosisNanoparticleTarget deliveryEGFP-EGF1 |
| spellingShingle | Wu Z Chen C Zhang B Tang L Shi W Liao D Di G Davis JR Wang H EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis International Journal of Nanomedicine Tissue factor Atherosclerosis Nanoparticle Target delivery EGFP-EGF1 |
| title | EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis |
| title_full | EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis |
| title_fullStr | EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis |
| title_full_unstemmed | EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis |
| title_short | EGFP-EGF1-conjugated poly(lactic-co-glycolic acid) nanoparticles, a new diagnostic tool and drug carrier for atherosclerosis |
| title_sort | egfp egf1 conjugated poly lactic co glycolic acid nanoparticles a new diagnostic tool and drug carrier for atherosclerosis |
| topic | Tissue factor Atherosclerosis Nanoparticle Target delivery EGFP-EGF1 |
| url | https://www.dovepress.com/egfp-egf1-conjugated-polylactic-co-glycolic-acid-nanoparticles-a-new-d-peer-reviewed-article-IJN |
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