Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i>
Providing simultaneously autotrophic and heterotrophic carbon sources is a promising strategy to overcome the limits of autotrophic syngas fermentations. D-xylose and L-arabinose are particularly interesting as they can be obtained by the hydrolysis of lignocellulosic biomass. The individual convers...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/2/102 |
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| author | Anne Oppelt Anton Rückel Markus Rupp Dirk Weuster-Botz |
| author_facet | Anne Oppelt Anton Rückel Markus Rupp Dirk Weuster-Botz |
| author_sort | Anne Oppelt |
| collection | DOAJ |
| description | Providing simultaneously autotrophic and heterotrophic carbon sources is a promising strategy to overcome the limits of autotrophic syngas fermentations. D-xylose and L-arabinose are particularly interesting as they can be obtained by the hydrolysis of lignocellulosic biomass. The individual conversion of varying initial concentrations of these pentoses and D-fructose as reference was studied with <i>C. autoethanogenum</i> in fully controlled stirred-tank reactors with a continuous syngas supply. All mixotrophic batch processes showed increased biomass and product formation compared to an autotrophic reference process. Simultaneous CO and D-xylose or L-arabinose conversion was observed in contrast to D-fructose. In the mixotrophic batch processes with L-arabinose or D-xylose, the simultaneous CO and sugar conversion resulted in high final alcohol-to-acid ratios of up to 58 g g<sup>−1</sup>. L-arabinose was superior as a mixotrophic carbon source because biomass and alcohol concentrations (ethanol and 2,3-butanediol) were highest, and significant amounts of <i>meso</i>-2,3-butanediol (>1 g L<sup>−1</sup>) in addition to D-2,3-butanediol (>2 g L<sup>−1</sup>) were solely produced with L-arabinose. Furthermore, <i>C. autoethanogenum</i> could not produce <i>meso</i>-2,3 butanediol under purely heterotrophic conditions. The mixotrophic production of <i>meso</i>-2,3-butanediol from L-arabinose and syngas, both available from residual lignocellulosic biomass, is very promising for use as a monomer for bio-based polyurethanes or as an antiseptic agent. |
| first_indexed | 2024-03-07T22:33:57Z |
| format | Article |
| id | doaj.art-cc59d91b19c249b8a665b9aa04769dbf |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-07T22:33:57Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-cc59d91b19c249b8a665b9aa04769dbf2024-02-23T15:15:57ZengMDPI AGFermentation2311-56372024-02-0110210210.3390/fermentation10020102Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i>Anne Oppelt0Anton Rückel1Markus Rupp2Dirk Weuster-Botz3Chair of Biochemical Engineering, School of Engineering and Design, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, GermanyChair of Biochemical Engineering, School of Engineering and Design, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, GermanyChair of Biochemical Engineering, School of Engineering and Design, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, GermanyChair of Biochemical Engineering, School of Engineering and Design, Technical University of Munich, Boltzmannstr. 15, 85748 Garching, GermanyProviding simultaneously autotrophic and heterotrophic carbon sources is a promising strategy to overcome the limits of autotrophic syngas fermentations. D-xylose and L-arabinose are particularly interesting as they can be obtained by the hydrolysis of lignocellulosic biomass. The individual conversion of varying initial concentrations of these pentoses and D-fructose as reference was studied with <i>C. autoethanogenum</i> in fully controlled stirred-tank reactors with a continuous syngas supply. All mixotrophic batch processes showed increased biomass and product formation compared to an autotrophic reference process. Simultaneous CO and D-xylose or L-arabinose conversion was observed in contrast to D-fructose. In the mixotrophic batch processes with L-arabinose or D-xylose, the simultaneous CO and sugar conversion resulted in high final alcohol-to-acid ratios of up to 58 g g<sup>−1</sup>. L-arabinose was superior as a mixotrophic carbon source because biomass and alcohol concentrations (ethanol and 2,3-butanediol) were highest, and significant amounts of <i>meso</i>-2,3-butanediol (>1 g L<sup>−1</sup>) in addition to D-2,3-butanediol (>2 g L<sup>−1</sup>) were solely produced with L-arabinose. Furthermore, <i>C. autoethanogenum</i> could not produce <i>meso</i>-2,3 butanediol under purely heterotrophic conditions. The mixotrophic production of <i>meso</i>-2,3-butanediol from L-arabinose and syngas, both available from residual lignocellulosic biomass, is very promising for use as a monomer for bio-based polyurethanes or as an antiseptic agent.https://www.mdpi.com/2311-5637/10/2/102<i>Clostridium autoethanogenum</i>syngas fermentationmixotrophic alcohol productioncarbon monoxide conversionL-arabinose<i>meso</i>-2,3-butandiol |
| spellingShingle | Anne Oppelt Anton Rückel Markus Rupp Dirk Weuster-Botz Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> Fermentation <i>Clostridium autoethanogenum</i> syngas fermentation mixotrophic alcohol production carbon monoxide conversion L-arabinose <i>meso</i>-2,3-butandiol |
| title | Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> |
| title_full | Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> |
| title_fullStr | Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> |
| title_full_unstemmed | Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> |
| title_short | Mixotrophic Syngas Conversion Enables the Production of <i>meso</i>-2,3-butanediol with <i>Clostridium autoethanogenum</i> |
| title_sort | mixotrophic syngas conversion enables the production of i meso i 2 3 butanediol with i clostridium autoethanogenum i |
| topic | <i>Clostridium autoethanogenum</i> syngas fermentation mixotrophic alcohol production carbon monoxide conversion L-arabinose <i>meso</i>-2,3-butandiol |
| url | https://www.mdpi.com/2311-5637/10/2/102 |
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