<i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study
Antimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a “One Health” approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the sprea...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/4/966 |
| _version_ | 1797539426772975616 |
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| author | Cristian Piras Pierluigi Aldo Di Ciccio Alessio Soggiu Viviana Greco Bruno Tilocca Nicola Costanzo Carlotta Ceniti Andrea Urbani Luigi Bonizzi Adriana Ianieri Paola Roncada |
| author_facet | Cristian Piras Pierluigi Aldo Di Ciccio Alessio Soggiu Viviana Greco Bruno Tilocca Nicola Costanzo Carlotta Ceniti Andrea Urbani Luigi Bonizzi Adriana Ianieri Paola Roncada |
| author_sort | Cristian Piras |
| collection | DOAJ |
| description | Antimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a “One Health” approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the spread of AMR. One of the most common ways for bacteria to live is to adhere to surfaces and form biofilms. <i>Staphylococcus aureus</i> (<i>S. aureus</i>) can form biofilm on most surfaces and in a wide heterogeneity of environmental conditions. The biofilm guarantees the survival of the <i>S. aureus</i> in harsh environmental conditions and represents an issue for the food industry and animal production. The identification and characterization of biofilm-related proteins may provide interesting insights into biofilm formation mechanisms in <i>S. aureus</i>. In this regard, the aims of this study were: (i) to use proteomics to compare proteomes of <i>S. aureus</i> growing in planktonic and biofilm forms in order to investigate the common features of biofilm formation properties of different strains; (ii) to identify specific biofilm mechanisms that may be involved in AMR. The proteomic analysis showed 14 differentially expressed proteins among biofilm and planktonic forms of <i>S. aureus</i>. Moreover, three proteins, such as alcohol dehydrogenase, ATP-dependent 6-phosphofructokinase, and fructose-bisphosphate aldolase, were only differentially expressed in strains classified as high biofilm producers. Differentially regulated catabolites metabolisms and the switch to lower oxygen-related metabolisms were related to the sessile conformation analyzed. |
| first_indexed | 2024-03-10T12:45:54Z |
| format | Article |
| id | doaj.art-cc5ab12c38624aaf8c3edbf78c5803b2 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T12:45:54Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-cc5ab12c38624aaf8c3edbf78c5803b22023-11-21T13:30:53ZengMDPI AGAnimals2076-26152021-03-0111496610.3390/ani11040966<i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic StudyCristian Piras0Pierluigi Aldo Di Ciccio1Alessio Soggiu2Viviana Greco3Bruno Tilocca4Nicola Costanzo5Carlotta Ceniti6Andrea Urbani7Luigi Bonizzi8Adriana Ianieri9Paola Roncada10Department of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, Grugliasco, 10095 Torino, ItalySurgical and Dental Sciences-One Health Unit, Department of Biomedical, University of Milano, Via Celoria 10, 20133 Milano, ItalyDepartment of Basic Biotechnological Sciences, Intensivological and Perioperative Clinics, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyDepartment of Basic Biotechnological Sciences, Intensivological and Perioperative Clinics, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Roma, ItalySurgical and Dental Sciences-One Health Unit, Department of Biomedical, University of Milano, Via Celoria 10, 20133 Milano, ItalyDeparment of Food and Drug, University of Parma, Parco Area delle Scienze 27A, 43124 Parma, ItalyDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Campus Universitario “S. Venuta”, Viale Europa, I-88100 Catanzaro, ItalyAntimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a “One Health” approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the spread of AMR. One of the most common ways for bacteria to live is to adhere to surfaces and form biofilms. <i>Staphylococcus aureus</i> (<i>S. aureus</i>) can form biofilm on most surfaces and in a wide heterogeneity of environmental conditions. The biofilm guarantees the survival of the <i>S. aureus</i> in harsh environmental conditions and represents an issue for the food industry and animal production. The identification and characterization of biofilm-related proteins may provide interesting insights into biofilm formation mechanisms in <i>S. aureus</i>. In this regard, the aims of this study were: (i) to use proteomics to compare proteomes of <i>S. aureus</i> growing in planktonic and biofilm forms in order to investigate the common features of biofilm formation properties of different strains; (ii) to identify specific biofilm mechanisms that may be involved in AMR. The proteomic analysis showed 14 differentially expressed proteins among biofilm and planktonic forms of <i>S. aureus</i>. Moreover, three proteins, such as alcohol dehydrogenase, ATP-dependent 6-phosphofructokinase, and fructose-bisphosphate aldolase, were only differentially expressed in strains classified as high biofilm producers. Differentially regulated catabolites metabolisms and the switch to lower oxygen-related metabolisms were related to the sessile conformation analyzed.https://www.mdpi.com/2076-2615/11/4/966<i>Staphylococcus aureus</i>planktonic cellsbiofilmproteomicsfood safetyantimicrobial resistance |
| spellingShingle | Cristian Piras Pierluigi Aldo Di Ciccio Alessio Soggiu Viviana Greco Bruno Tilocca Nicola Costanzo Carlotta Ceniti Andrea Urbani Luigi Bonizzi Adriana Ianieri Paola Roncada <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study Animals <i>Staphylococcus aureus</i> planktonic cells biofilm proteomics food safety antimicrobial resistance |
| title | <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study |
| title_full | <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study |
| title_fullStr | <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study |
| title_full_unstemmed | <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study |
| title_short | <i>S. aureus</i> Biofilm Protein Expression Linked to Antimicrobial Resistance: A Proteomic Study |
| title_sort | i s aureus i biofilm protein expression linked to antimicrobial resistance a proteomic study |
| topic | <i>Staphylococcus aureus</i> planktonic cells biofilm proteomics food safety antimicrobial resistance |
| url | https://www.mdpi.com/2076-2615/11/4/966 |
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