Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times
The current research was characterized on phenolic metabolite profile including six chemical structures (phenolic acid, luteolin, orientin, apigenin, isoscoparin, and tricin) in wheat seedlings using HPLC-Q-Orbitrap-MS/MS and NMR techniques. Our study was also was the first to demonstrate fluctuatio...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157522003558 |
| _version_ | 1797868318524178432 |
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| author | HanGyeol Lee Ji Yeong Yang Ji Eun Ra Hyung-Jae Ahn Mi Ja Lee Hyun Young Kim Seung-Yeob Song Du Hyun Kim Jin Hwan Lee Woo Duck Seo |
| author_facet | HanGyeol Lee Ji Yeong Yang Ji Eun Ra Hyung-Jae Ahn Mi Ja Lee Hyun Young Kim Seung-Yeob Song Du Hyun Kim Jin Hwan Lee Woo Duck Seo |
| author_sort | HanGyeol Lee |
| collection | DOAJ |
| description | The current research was characterized on phenolic metabolite profile including six chemical structures (phenolic acid, luteolin, orientin, apigenin, isoscoparin, and tricin) in wheat seedlings using HPLC-Q-Orbitrap-MS/MS and NMR techniques. Our study was also was the first to demonstrate fluctuations of isolated nine phenolic contents and antioxidant properties in various cultivars of this species with different growth times. The antioxidant abilities differed significantly in the 80 % methanol extracts (600 μg/mL) according to cultivar and growth time, with the highest average activities (DPPH: 82 %; ABTS: 87 %) observed after 7 days. The isolated nine compositions exhibited considerable differences in cultivars and growth times, specifically, isoorientin (6) and isochaftoside (8) were observed the most abundant average contents (99.3; 64.3 mg/100 g), representing approximately 28.3 and 18.3 % (total content: 350.8 mg/100 g). Their total phenolics showed the highest rates (420.8 mg/100 g) at 7 days, followed by 9 → 5 → 12 → 14 days with 374.6 → 366.7 → 350.7 → 241.1 mg/100 g, as the rank orders of antioxidant effects. These findings suggest that wheat seedlings may be a potent source of functional agents. |
| first_indexed | 2024-04-09T23:54:00Z |
| format | Article |
| id | doaj.art-cc5b98ab1162460fbeae5f821d124f43 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-09T23:54:00Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-cc5b98ab1162460fbeae5f821d124f432023-03-17T04:34:13ZengElsevierFood Chemistry: X2590-15752023-03-0117100557Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth timesHanGyeol Lee0Ji Yeong Yang1Ji Eun Ra2Hyung-Jae Ahn3Mi Ja Lee4Hyun Young Kim5Seung-Yeob Song6Du Hyun Kim7Jin Hwan Lee8Woo Duck Seo9Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of KoreaDepartment of Life Resources Industry, Dong-A University, Busan 49315, Republic of KoreaDepartment of Life Resources Industry, Dong-A University, Busan 49315, Republic of Korea; Corresponding authors.Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of Korea; Corresponding authors.The current research was characterized on phenolic metabolite profile including six chemical structures (phenolic acid, luteolin, orientin, apigenin, isoscoparin, and tricin) in wheat seedlings using HPLC-Q-Orbitrap-MS/MS and NMR techniques. Our study was also was the first to demonstrate fluctuations of isolated nine phenolic contents and antioxidant properties in various cultivars of this species with different growth times. The antioxidant abilities differed significantly in the 80 % methanol extracts (600 μg/mL) according to cultivar and growth time, with the highest average activities (DPPH: 82 %; ABTS: 87 %) observed after 7 days. The isolated nine compositions exhibited considerable differences in cultivars and growth times, specifically, isoorientin (6) and isochaftoside (8) were observed the most abundant average contents (99.3; 64.3 mg/100 g), representing approximately 28.3 and 18.3 % (total content: 350.8 mg/100 g). Their total phenolics showed the highest rates (420.8 mg/100 g) at 7 days, followed by 9 → 5 → 12 → 14 days with 374.6 → 366.7 → 350.7 → 241.1 mg/100 g, as the rank orders of antioxidant effects. These findings suggest that wheat seedlings may be a potent source of functional agents.http://www.sciencedirect.com/science/article/pii/S2590157522003558Wheat seedlingPhenolic metaboliteAntioxidantGrowth timeNMRHPLC-Q-Orbitrap-MS/MS |
| spellingShingle | HanGyeol Lee Ji Yeong Yang Ji Eun Ra Hyung-Jae Ahn Mi Ja Lee Hyun Young Kim Seung-Yeob Song Du Hyun Kim Jin Hwan Lee Woo Duck Seo Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times Food Chemistry: X Wheat seedling Phenolic metabolite Antioxidant Growth time NMR HPLC-Q-Orbitrap-MS/MS |
| title | Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times |
| title_full | Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times |
| title_fullStr | Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times |
| title_full_unstemmed | Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times |
| title_short | Elucidation of phenolic metabolites in wheat seedlings (Triticum aestivum L.) by NMR and HPLC-Q-Orbitrap-MS/MS: Changes in isolated phenolics and antioxidant effects through diverse growth times |
| title_sort | elucidation of phenolic metabolites in wheat seedlings triticum aestivum l by nmr and hplc q orbitrap ms ms changes in isolated phenolics and antioxidant effects through diverse growth times |
| topic | Wheat seedling Phenolic metabolite Antioxidant Growth time NMR HPLC-Q-Orbitrap-MS/MS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157522003558 |
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