Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever
Objective: A prospective evaluation of a rapid diagnostic test Dot EIA (Typhidot) for typhoid fever. Study design & duration: This prospective study was undertaken in a tertiary hospital from April 2007 to July 2008. Research Methodology: The patients were divided into three groups as I, II &am...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dow University of Health Sciences
2009-12-01
|
| Series: | Journal of the Dow University of Health Sciences |
| Subjects: | |
| Online Access: | https://www.jduhs.com/index.php/jduhs/article/view/1348 |
| _version_ | 1827939515365326848 |
|---|---|
| author | Haji Khan Khoharo Shuaib Ansari Fatima Qureshi |
| author_facet | Haji Khan Khoharo Shuaib Ansari Fatima Qureshi |
| author_sort | Haji Khan Khoharo |
| collection | DOAJ |
| description |
Objective: A prospective evaluation of a rapid diagnostic test Dot EIA (Typhidot) for typhoid fever. Study design & duration: This prospective study was undertaken in a tertiary hospital from April 2007 to July 2008.
Research Methodology: The patients were divided into three groups as I, II & III with typhoid fever, typhoid suspects & non-typhoid febrile illnesses respectively. Blood culture and typhidot tests were done for the subjects included in the study. The validity of the typhidot test was evaluated by determ ining the sensitivity, specificity, positive and negative predictive value.
Results: The mean age in years was 28±19 (SD). Out of 150 subjects, males were more than females. The typhidot IgM yielded very high sensitivity, specificity and a negative predictive value noted in group I = 93% & 98.80% 93% respectively and in group II= 79%, 98.80%, & 83% respectively where as in group III (non-typhoid febrile controls), the IgM antibodies were not detected at all (p=0.01). Sensitivity, specificity, and negative predictive value for typhidot IgG noted in group I = 73%, 63%, & 22.4% respectively and in group II = 82%, 37%, & 68% respectively (p=0.03) and a false positive reading of 63% was noted in group III (controls).
Conclusions: Typhidot test is a valid tool in the diagnosis of typhoid fever but a reliable and valid interpretation should be based on positive IgM.
|
| first_indexed | 2024-03-13T08:55:46Z |
| format | Article |
| id | doaj.art-cc5e48bcbe074238b626ebd069d797a7 |
| institution | Directory Open Access Journal |
| issn | 1995-2198 2410-2180 |
| language | English |
| last_indexed | 2024-03-13T08:55:46Z |
| publishDate | 2009-12-01 |
| publisher | Dow University of Health Sciences |
| record_format | Article |
| series | Journal of the Dow University of Health Sciences |
| spelling | doaj.art-cc5e48bcbe074238b626ebd069d797a72023-05-29T03:11:51ZengDow University of Health SciencesJournal of the Dow University of Health Sciences1995-21982410-21802009-12-0133Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid FeverHaji Khan Khoharo0Shuaib Ansari1Fatima Qureshi2 Department of Medicine, Muhammad Medical College Hospital, Mirpurkhas, Pakistan.Department of Medicine, Liaquat University Hospital Hyderabad/ Jamshoro, Pakistan.Department of Medicine, Muhammad Medical College Hospital, Mirpurkhas, Pakistan. Objective: A prospective evaluation of a rapid diagnostic test Dot EIA (Typhidot) for typhoid fever. Study design & duration: This prospective study was undertaken in a tertiary hospital from April 2007 to July 2008. Research Methodology: The patients were divided into three groups as I, II & III with typhoid fever, typhoid suspects & non-typhoid febrile illnesses respectively. Blood culture and typhidot tests were done for the subjects included in the study. The validity of the typhidot test was evaluated by determ ining the sensitivity, specificity, positive and negative predictive value. Results: The mean age in years was 28±19 (SD). Out of 150 subjects, males were more than females. The typhidot IgM yielded very high sensitivity, specificity and a negative predictive value noted in group I = 93% & 98.80% 93% respectively and in group II= 79%, 98.80%, & 83% respectively where as in group III (non-typhoid febrile controls), the IgM antibodies were not detected at all (p=0.01). Sensitivity, specificity, and negative predictive value for typhidot IgG noted in group I = 73%, 63%, & 22.4% respectively and in group II = 82%, 37%, & 68% respectively (p=0.03) and a false positive reading of 63% was noted in group III (controls). Conclusions: Typhidot test is a valid tool in the diagnosis of typhoid fever but a reliable and valid interpretation should be based on positive IgM. https://www.jduhs.com/index.php/jduhs/article/view/1348Salmonella typhi Dot- EIA (typhidot)typhoid fever |
| spellingShingle | Haji Khan Khoharo Shuaib Ansari Fatima Qureshi Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever Journal of the Dow University of Health Sciences Salmonella typhi Dot- EIA (typhidot)typhoid fever |
| title | Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever |
| title_full | Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever |
| title_fullStr | Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever |
| title_full_unstemmed | Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever |
| title_short | Prospective Evaluation of a Rapid Diagnostic Test Dot EIA (Typhidot) for Typhoid Fever |
| title_sort | prospective evaluation of a rapid diagnostic test dot eia typhidot for typhoid fever |
| topic | Salmonella typhi Dot- EIA (typhidot)typhoid fever |
| url | https://www.jduhs.com/index.php/jduhs/article/view/1348 |
| work_keys_str_mv | AT hajikhankhoharo prospectiveevaluationofarapiddiagnostictestdoteiatyphidotfortyphoidfever AT shuaibansari prospectiveevaluationofarapiddiagnostictestdoteiatyphidotfortyphoidfever AT fatimaqureshi prospectiveevaluationofarapiddiagnostictestdoteiatyphidotfortyphoidfever |