Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath
High concentrations of H2O2, indicative of increased oxidative stress in the lung, are observed in the exhaled breath of individuals affected by different respiratory diseases. Therefore, measuring H2O2 in exhaled breath represents a promising and non-invasive approach for monitoring the onset and p...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137024000402 |
| _version_ | 1797214840032329728 |
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| author | M.G. Bruno B. Patella M. Ferraro S. Di Vincenzo P. Pinto C. Torino A. Vilasi M.R. Giuffrè V.B. Juska A. O'Riordan R. Inguanta C. Cipollina E. Pace G. Aiello |
| author_facet | M.G. Bruno B. Patella M. Ferraro S. Di Vincenzo P. Pinto C. Torino A. Vilasi M.R. Giuffrè V.B. Juska A. O'Riordan R. Inguanta C. Cipollina E. Pace G. Aiello |
| author_sort | M.G. Bruno |
| collection | DOAJ |
| description | High concentrations of H2O2, indicative of increased oxidative stress in the lung, are observed in the exhaled breath of individuals affected by different respiratory diseases. Therefore, measuring H2O2 in exhaled breath represents a promising and non-invasive approach for monitoring the onset and progression of these diseases. Herein, we have developed an innovative, inexpensive, and easy-to-use device for the measurement of H2O2 in exhaled breath. The device is based on a silver layer covered with an electrodeposited thin film of chitosan, that ensures the wettability of the sensor in a humid atmosphere. The s-ensor was calibrated in the aerosol phase using both phosphate buffer solution and cell culture medium. In the buffer, a sensitivity of 0.110 ± 0.0042 μA μM−1 cm−2 (RSD: 4%) and a limit of detection of 30 μM were calculated, while in the cell culture medium, a sensitivity of 0.098 ± 0.0022 μA μM−1 cm−2 (RSD 2%) and a limit of detection of 40 μM were obtained. High selectivity to different interfering species was also verified. The sensor was further tested versus an aerosol phase obtained by nebulizing the culture medium derived from human bronchial epithelial cells that had been exposed to pro-oxidant and antioxidant treatments. The results were comparable with those obtained using the conventional cytofluorimetric method. Finally, sensor was tested in real exhaled breath samples and even after undergoing physical deformations. Data herein presented support that in future applications this device can be integrated into face masks allowing for easy breath monitoring. |
| first_indexed | 2024-04-24T11:20:33Z |
| format | Article |
| id | doaj.art-cc5f5446d63a476db43047e66c1a4329 |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-24T11:20:33Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-cc5f5446d63a476db43047e66c1a43292024-04-11T04:41:53ZengElsevierBiosensors and Bioelectronics: X2590-13702024-06-0118100476Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breathM.G. Bruno0B. Patella1M. Ferraro2S. Di Vincenzo3P. Pinto4C. Torino5A. Vilasi6M.R. Giuffrè7V.B. Juska8A. O'Riordan9R. Inguanta10C. Cipollina11E. Pace12G. Aiello13Dipartimento di Ingegneria, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyDipartimento di Ingegneria, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyInstitute of Translational Pharmacology, National Research Council, 90146 Palermo, ItalyInstitute of Translational Pharmacology, National Research Council, 90146 Palermo, ItalyInstitute of Translational Pharmacology, National Research Council, 90146 Palermo, Italy; Department of Public Health, Experimental and Forensic Medicine, University of Pavia, Pavia, 27100, ItalyInstitute of Clinical Physiology, National Research Council, 89124 Reggio Calabria, ItalyInstitute of Clinical Physiology, National Research Council, 89124 Reggio Calabria, ItalyRi.MED Foundation, 90133 Palermo, ItalyNanotechnology Group, Tyndall National Institute, University College Cork, Cork, T12 R5CP, IrelandNanotechnology Group, Tyndall National Institute, University College Cork, Cork, T12 R5CP, IrelandDipartimento di Ingegneria, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, Italy; Institute of Translational Pharmacology, National Research Council, 90146 Palermo, Italy; Corresponding author. Dipartimento di Ingegneria, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, Italy.Ri.MED Foundation, 90133 Palermo, Italy; Institute of Translational Pharmacology, National Research Council, 90146 Palermo, ItalyInstitute of Translational Pharmacology, National Research Council, 90146 Palermo, ItalyDipartimento di Ingegneria, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, ItalyHigh concentrations of H2O2, indicative of increased oxidative stress in the lung, are observed in the exhaled breath of individuals affected by different respiratory diseases. Therefore, measuring H2O2 in exhaled breath represents a promising and non-invasive approach for monitoring the onset and progression of these diseases. Herein, we have developed an innovative, inexpensive, and easy-to-use device for the measurement of H2O2 in exhaled breath. The device is based on a silver layer covered with an electrodeposited thin film of chitosan, that ensures the wettability of the sensor in a humid atmosphere. The s-ensor was calibrated in the aerosol phase using both phosphate buffer solution and cell culture medium. In the buffer, a sensitivity of 0.110 ± 0.0042 μA μM−1 cm−2 (RSD: 4%) and a limit of detection of 30 μM were calculated, while in the cell culture medium, a sensitivity of 0.098 ± 0.0022 μA μM−1 cm−2 (RSD 2%) and a limit of detection of 40 μM were obtained. High selectivity to different interfering species was also verified. The sensor was further tested versus an aerosol phase obtained by nebulizing the culture medium derived from human bronchial epithelial cells that had been exposed to pro-oxidant and antioxidant treatments. The results were comparable with those obtained using the conventional cytofluorimetric method. Finally, sensor was tested in real exhaled breath samples and even after undergoing physical deformations. Data herein presented support that in future applications this device can be integrated into face masks allowing for easy breath monitoring.http://www.sciencedirect.com/science/article/pii/S2590137024000402Electrochemical sensorsHuman bronchial epithelial cellsOxidative stressReal-time monitoringWorkers' health and safetyWearable sensors |
| spellingShingle | M.G. Bruno B. Patella M. Ferraro S. Di Vincenzo P. Pinto C. Torino A. Vilasi M.R. Giuffrè V.B. Juska A. O'Riordan R. Inguanta C. Cipollina E. Pace G. Aiello Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath Biosensors and Bioelectronics: X Electrochemical sensors Human bronchial epithelial cells Oxidative stress Real-time monitoring Workers' health and safety Wearable sensors |
| title | Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath |
| title_full | Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath |
| title_fullStr | Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath |
| title_full_unstemmed | Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath |
| title_short | Wearable sensor for real-time monitoring of oxidative stress in simulated exhaled breath |
| title_sort | wearable sensor for real time monitoring of oxidative stress in simulated exhaled breath |
| topic | Electrochemical sensors Human bronchial epithelial cells Oxidative stress Real-time monitoring Workers' health and safety Wearable sensors |
| url | http://www.sciencedirect.com/science/article/pii/S2590137024000402 |
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