Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants
Introduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein. Purpose. To determine the antigenic identity of immunodominant proteins of different...
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| Format: | Article |
| Language: | Russian |
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Central Research Institute for Epidemiology
2023-05-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
| Subjects: | |
| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/11153/869 |
| _version_ | 1797629306922336256 |
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| author | Margarita P. Izhberdeeva Anastasiya A. Sautkina Irina A. Barkova Dmitry V. Viktorov |
| author_facet | Margarita P. Izhberdeeva Anastasiya A. Sautkina Irina A. Barkova Dmitry V. Viktorov |
| author_sort | Margarita P. Izhberdeeva |
| collection | DOAJ |
| description | Introduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein.
Purpose. To determine the antigenic identity of immunodominant proteins of different genovariants of B. anthracis isolated by gel chromatography and electrophoresis.
Materials and methods. Culture filtrates of isogenic variants of B. anthracis strain 575/122 (pXO1+, pXO2+): R01 (pXO1+, pXO2); R00 (pXO1, pXO2) were used in the study. Gel chromatographic fractionation and electrophoretic separation were carried out according to standard methods. The antigenic properties of proteins isolated by gel chromatography and electrophoresis were studied by immunodiffusion with polyclonal monospecific sera against the protective antigen and the EA1 protein of the S-layer.
Results. Gel chromatographic separation of B. anthracis 575/122 culture filtrates R01 (pXO1+, pXO2) and R00 (pXO1, pXO2)yielded fractions 1 and 5. Sera against EA1 protein and antigens of fraction 1 of strains B. anthracis 575/122 R00 and B. anthracis 575/122 R01 culture filtrates identified the identical antigens. Serum against antigens of fraction 5 of B. anthracis 575/122 R01 contained antibodies to numerous proteins, including the protective antigen isolated by electrophoresis.
Discussion. The antigenic identity of immunodominant proteins isolated by gel chromatography and electrophoresis was identified.
Conclusion. EA1 and PA proteins isolated by electrophoresis and gel chromatography can be used for production of monoclonal and polyclonal monospecific antibodies suitable for the design of diagnostic preparations. |
| first_indexed | 2024-03-11T10:51:29Z |
| format | Article |
| id | doaj.art-cc61659562b64581962c8f518215b8e2 |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-03-11T10:51:29Z |
| publishDate | 2023-05-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-cc61659562b64581962c8f518215b8e22023-11-13T17:20:01ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132023-05-01100220320810.36233/0372-9311-284882Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariantsMargarita P. Izhberdeeva0https://orcid.org/0000-0002-2979-4452Anastasiya A. Sautkina1https://orcid.org/0000-0001-6302-4438Irina A. Barkova2https://orcid.org/0000-0002-5036-0034Dmitry V. Viktorov3https://orcid.org/0000-0002-2722-7948Volgograd Plague Control Research InstituteVolgograd Plague Control Research InstituteVolgograd Plague Control Research InstituteVolgograd Plague Control Research InstituteIntroduction. The main biological raw materials for the production of immunobiological preparations for identification of Bacillus anthracis are its specific antigens, the protective antigen and the EA1 protein. Purpose. To determine the antigenic identity of immunodominant proteins of different genovariants of B. anthracis isolated by gel chromatography and electrophoresis. Materials and methods. Culture filtrates of isogenic variants of B. anthracis strain 575/122 (pXO1+, pXO2+): R01 (pXO1+, pXO2); R00 (pXO1, pXO2) were used in the study. Gel chromatographic fractionation and electrophoretic separation were carried out according to standard methods. The antigenic properties of proteins isolated by gel chromatography and electrophoresis were studied by immunodiffusion with polyclonal monospecific sera against the protective antigen and the EA1 protein of the S-layer. Results. Gel chromatographic separation of B. anthracis 575/122 culture filtrates R01 (pXO1+, pXO2) and R00 (pXO1, pXO2)yielded fractions 1 and 5. Sera against EA1 protein and antigens of fraction 1 of strains B. anthracis 575/122 R00 and B. anthracis 575/122 R01 culture filtrates identified the identical antigens. Serum against antigens of fraction 5 of B. anthracis 575/122 R01 contained antibodies to numerous proteins, including the protective antigen isolated by electrophoresis. Discussion. The antigenic identity of immunodominant proteins isolated by gel chromatography and electrophoresis was identified. Conclusion. EA1 and PA proteins isolated by electrophoresis and gel chromatography can be used for production of monoclonal and polyclonal monospecific antibodies suitable for the design of diagnostic preparations.https://microbiol.crie.ru/jour/article/viewFile/11153/869bacillus anthracisprotective antigenea1 proteingel chromatographyelectrophoresisimmunodiffusion reactionantigenic identity |
| spellingShingle | Margarita P. Izhberdeeva Anastasiya A. Sautkina Irina A. Barkova Dmitry V. Viktorov Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants Журнал микробиологии, эпидемиологии и иммунобиологии bacillus anthracis protective antigen ea1 protein gel chromatography electrophoresis immunodiffusion reaction antigenic identity |
| title | Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants |
| title_full | Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants |
| title_fullStr | Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants |
| title_full_unstemmed | Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants |
| title_short | Antigenic identity of immunodominant proteins of <i>Bacillus anthracis</i> genovariants |
| title_sort | antigenic identity of immunodominant proteins of i bacillus anthracis i genovariants |
| topic | bacillus anthracis protective antigen ea1 protein gel chromatography electrophoresis immunodiffusion reaction antigenic identity |
| url | https://microbiol.crie.ru/jour/article/viewFile/11153/869 |
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