To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration
Oxidative stress has been suggested as an important factor in the progress of sarcopenia. The current treatments for sarcopenia have the disadvantages of insufficient effect or daily administration. Therefore, an alternative for effective, safety and long-term treatment may be a solution for unmet n...
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MDPI AG
2021-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/3/488 |
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| author | Ya-Jyun Liang Jia-Yu Hong I-Hsuan Yang Xin-Ran Zhou Yi-Wen Lin Tzu-Chieh Lin Chun-Han Hou Feng-Huei Lin |
| author_facet | Ya-Jyun Liang Jia-Yu Hong I-Hsuan Yang Xin-Ran Zhou Yi-Wen Lin Tzu-Chieh Lin Chun-Han Hou Feng-Huei Lin |
| author_sort | Ya-Jyun Liang |
| collection | DOAJ |
| description | Oxidative stress has been suggested as an important factor in the progress of sarcopenia. The current treatments for sarcopenia have the disadvantages of insufficient effect or daily administration. Therefore, an alternative for effective, safety and long-term treatment may be a solution for unmet needs. <i>Bletilla striata</i> polysaccharide has been reported to have antioxidative and anti-inflammatory properties. In this study, we used <i>Bletilla striata</i> polysaccharide (BSP) combined with hydroxyapatite, a carrier. We hypothesized that the resulting combination (BSP-HAP) is a good formula for the controlled release of BSP via intramuscular (IM) administration, so as to prevent the worsening of presarcopenia or even recover from the early stage of the illness. In this research, BSP-HAP was synthesized by a modified low temperature co-precipitation process that would be beneficial for BSP loading. By conducting DCFDA, WST-1 and the Live/Dead assay, BSP-HAP is shown to be a biocompatible material which may release BSP by cells through the endocytosis pathway. Animal studies revealed that the rats treated with BSP-HAP could effectively recover muscle endurance, grip strength or fat/lean mass ratio from lipopolysaccharide (LPS)-induced sarcopenia. This study shows BSP delivered by BSP-HAP system has potential for application in the treatment and prevention of sarcopenia in the future. |
| first_indexed | 2024-03-10T13:04:13Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T13:04:13Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-cc65af782f02455ab66ecfa52b84e9ca2023-11-21T11:19:02ZengMDPI AGAntioxidants2076-39212021-03-0110348810.3390/antiox10030488To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular AdministrationYa-Jyun Liang0Jia-Yu Hong1I-Hsuan Yang2Xin-Ran Zhou3Yi-Wen Lin4Tzu-Chieh Lin5Chun-Han Hou6Feng-Huei Lin7Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanDepartment of Orthopedic Surgery, National Taiwan University Hospital, Taipei 10617, TaiwanInstitute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 10617, TaiwanOxidative stress has been suggested as an important factor in the progress of sarcopenia. The current treatments for sarcopenia have the disadvantages of insufficient effect or daily administration. Therefore, an alternative for effective, safety and long-term treatment may be a solution for unmet needs. <i>Bletilla striata</i> polysaccharide has been reported to have antioxidative and anti-inflammatory properties. In this study, we used <i>Bletilla striata</i> polysaccharide (BSP) combined with hydroxyapatite, a carrier. We hypothesized that the resulting combination (BSP-HAP) is a good formula for the controlled release of BSP via intramuscular (IM) administration, so as to prevent the worsening of presarcopenia or even recover from the early stage of the illness. In this research, BSP-HAP was synthesized by a modified low temperature co-precipitation process that would be beneficial for BSP loading. By conducting DCFDA, WST-1 and the Live/Dead assay, BSP-HAP is shown to be a biocompatible material which may release BSP by cells through the endocytosis pathway. Animal studies revealed that the rats treated with BSP-HAP could effectively recover muscle endurance, grip strength or fat/lean mass ratio from lipopolysaccharide (LPS)-induced sarcopenia. This study shows BSP delivered by BSP-HAP system has potential for application in the treatment and prevention of sarcopenia in the future.https://www.mdpi.com/2076-3921/10/3/488sarcopeniaantioxidant<i>Bletilla striata</i> polysaccharide (BSP)hydroxyapatitedrug delivery |
| spellingShingle | Ya-Jyun Liang Jia-Yu Hong I-Hsuan Yang Xin-Ran Zhou Yi-Wen Lin Tzu-Chieh Lin Chun-Han Hou Feng-Huei Lin To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration Antioxidants sarcopenia antioxidant <i>Bletilla striata</i> polysaccharide (BSP) hydroxyapatite drug delivery |
| title | To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration |
| title_full | To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration |
| title_fullStr | To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration |
| title_full_unstemmed | To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration |
| title_short | To Synthesize Hydroxyapatite by Modified Low Temperature Method Loaded with <i>Bletilla striata</i> Polysaccharide as Antioxidant for the Prevention of Sarcopenia by Intramuscular Administration |
| title_sort | to synthesize hydroxyapatite by modified low temperature method loaded with i bletilla striata i polysaccharide as antioxidant for the prevention of sarcopenia by intramuscular administration |
| topic | sarcopenia antioxidant <i>Bletilla striata</i> polysaccharide (BSP) hydroxyapatite drug delivery |
| url | https://www.mdpi.com/2076-3921/10/3/488 |
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