Long-term preservation of modern Russian potato cultivars in the VIR cryobank
Cryopreservation of shoot-tips (apexes) excised from in vitro plants is used for long-term preservation of potato cultivars. The most widely used method for creating potato cryo-collections is droplet-vitrification of shoot-tips which, together with its numerous modifications, is widely used in gene...
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| Format: | Article |
| Language: | English |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2021-04-01
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| Series: | Биотехнология и селекция растений |
| Subjects: | |
| Online Access: | https://biosel.elpub.ru/jour/article/view/101 |
| _version_ | 1797324226229698560 |
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| author | O. S. Efremova N. N. Volkova T. A. Gavrilenko |
| author_facet | O. S. Efremova N. N. Volkova T. A. Gavrilenko |
| author_sort | O. S. Efremova |
| collection | DOAJ |
| description | Cryopreservation of shoot-tips (apexes) excised from in vitro plants is used for long-term preservation of potato cultivars. The most widely used method for creating potato cryo-collections is droplet-vitrification of shoot-tips which, together with its numerous modifications, is widely used in genebanks. A modified protocol of droplet vitrification method is used at VIR for cryopreservation of shoot tips from in vitro potato plants. This paper presents the results of cryopreservation of modern cultivars which were released by seven Russian breeding centers. In vitro clones used in the cryopreservation experiments were genetically identical to the cultivars’ nomenclatural standards and herbarium vouchers.The frequency of post-thaw regeneration in control experiments after short-term cryopreservation varied from 23.3 to 53.3%, depending on the genotype. Five out of 16 accessions (‘Varâg’, ‘Gusar’, ‘Evpatij’, ‘Solnečnyj’, ‘Tango’)* had low post-cryogenic regenerative capacity from 20 to 30%; the regeneration rate exceeded 30% in 11 accessions, and 8 cultivars (‘Grand’, ‘Zlatka’, ‘Lina’, ‘Safo’, ‘Siverskij’, ‘Signal’, ‘Utro’, ‘Ûna’) and ‘Аlyj Parus’ breeding clone had regeneration rate above 40%. The regeneration rate in the studied subset was genotype independent according to the ANOVA results (p=0.711). Viability and regeneration rate were significantly correlated (r=0.86). As a result of the experiments, explants of 14 modern cultivars and two breeding clones with the known post-thaw regeneration rate were successfully cryopreserved in the VIR cryobank. Four cultivars (‘Grand’, ‘Gusar’, ‘Signal’, ‘Utro’) were monitored for their regeneration capacity after the long-term (seven months) preservation in the VIR cryobank. On an average, these four cultivars demonstrated a post-thaw regeneration capacity of 41.8%. It can be concluded that the use of the modified method of droplet vitrification is relevant for increasing the VIR potato cryo-collection. |
| first_indexed | 2024-03-08T05:53:01Z |
| format | Article |
| id | doaj.art-cc67322c573945c08f1f0067c02427f3 |
| institution | Directory Open Access Journal |
| issn | 2658-6266 2658-6258 |
| language | English |
| last_indexed | 2024-03-08T05:53:01Z |
| publishDate | 2021-04-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Биотехнология и селекция растений |
| spelling | doaj.art-cc67322c573945c08f1f0067c02427f32024-02-05T06:53:48ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesБиотехнология и селекция растений2658-62662658-62582021-04-0133687610.30901/2658-6266-2020-3-o156Long-term preservation of modern Russian potato cultivars in the VIR cryobankO. S. Efremova0N. N. Volkova1T. A. Gavrilenko2N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR)Cryopreservation of shoot-tips (apexes) excised from in vitro plants is used for long-term preservation of potato cultivars. The most widely used method for creating potato cryo-collections is droplet-vitrification of shoot-tips which, together with its numerous modifications, is widely used in genebanks. A modified protocol of droplet vitrification method is used at VIR for cryopreservation of shoot tips from in vitro potato plants. This paper presents the results of cryopreservation of modern cultivars which were released by seven Russian breeding centers. In vitro clones used in the cryopreservation experiments were genetically identical to the cultivars’ nomenclatural standards and herbarium vouchers.The frequency of post-thaw regeneration in control experiments after short-term cryopreservation varied from 23.3 to 53.3%, depending on the genotype. Five out of 16 accessions (‘Varâg’, ‘Gusar’, ‘Evpatij’, ‘Solnečnyj’, ‘Tango’)* had low post-cryogenic regenerative capacity from 20 to 30%; the regeneration rate exceeded 30% in 11 accessions, and 8 cultivars (‘Grand’, ‘Zlatka’, ‘Lina’, ‘Safo’, ‘Siverskij’, ‘Signal’, ‘Utro’, ‘Ûna’) and ‘Аlyj Parus’ breeding clone had regeneration rate above 40%. The regeneration rate in the studied subset was genotype independent according to the ANOVA results (p=0.711). Viability and regeneration rate were significantly correlated (r=0.86). As a result of the experiments, explants of 14 modern cultivars and two breeding clones with the known post-thaw regeneration rate were successfully cryopreserved in the VIR cryobank. Four cultivars (‘Grand’, ‘Gusar’, ‘Signal’, ‘Utro’) were monitored for their regeneration capacity after the long-term (seven months) preservation in the VIR cryobank. On an average, these four cultivars demonstrated a post-thaw regeneration capacity of 41.8%. It can be concluded that the use of the modified method of droplet vitrification is relevant for increasing the VIR potato cryo-collection.https://biosel.elpub.ru/jour/article/view/101cryopreservation<i>solanum tuberosum</i>released cultivars |
| spellingShingle | O. S. Efremova N. N. Volkova T. A. Gavrilenko Long-term preservation of modern Russian potato cultivars in the VIR cryobank Биотехнология и селекция растений cryopreservation <i>solanum tuberosum</i> released cultivars |
| title | Long-term preservation of modern Russian potato cultivars in the VIR cryobank |
| title_full | Long-term preservation of modern Russian potato cultivars in the VIR cryobank |
| title_fullStr | Long-term preservation of modern Russian potato cultivars in the VIR cryobank |
| title_full_unstemmed | Long-term preservation of modern Russian potato cultivars in the VIR cryobank |
| title_short | Long-term preservation of modern Russian potato cultivars in the VIR cryobank |
| title_sort | long term preservation of modern russian potato cultivars in the vir cryobank |
| topic | cryopreservation <i>solanum tuberosum</i> released cultivars |
| url | https://biosel.elpub.ru/jour/article/view/101 |
| work_keys_str_mv | AT osefremova longtermpreservationofmodernrussianpotatocultivarsinthevircryobank AT nnvolkova longtermpreservationofmodernrussianpotatocultivarsinthevircryobank AT tagavrilenko longtermpreservationofmodernrussianpotatocultivarsinthevircryobank |