High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury
Background: microRNAs (miRNAs) are closely associated with the pathogenesis of various diseases, but the relationship between miRNAs and myocardial ischemia-reperfusion (I/R) injury remains unclear. Therefore, we aimed to explore the role and function of miRNAs and identify target genes regulating I...
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| Format: | Article |
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IMR Press
2024-01-01
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| Series: | Frontiers in Bioscience-Landmark |
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| Online Access: | https://www.imrpress.com/journal/FBL/29/1/10.31083/j.fbl2901038 |
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| author | Senjie Li Dongqing Lv Yan Lu Yanwei Zhang Yongping Jia |
| author_facet | Senjie Li Dongqing Lv Yan Lu Yanwei Zhang Yongping Jia |
| author_sort | Senjie Li |
| collection | DOAJ |
| description | Background: microRNAs (miRNAs) are closely associated with the pathogenesis of various diseases, but the relationship between miRNAs and myocardial ischemia-reperfusion (I/R) injury remains unclear. Therefore, we aimed to explore the role and function of miRNAs and identify target genes regulating I/R. Methods: We established a hypoxia/reoxygenation (H/R) model to detect differentially expressed miRNAs using high-throughput sequencing in rat myocardial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were used to analyze the potential functions and signaling pathways of target genes. Results: We identified 113 differentially expressed miRNAs, comprising 76 and 37 upregulated and downregulated genes, respectively. Database predictions suggested that miR-200a-3p may act through the ferroptosis pathway, and we assessed the expression of miR-200a-3p, iron ions, and ferroptosis markers. The expression of miR-200a-3p significantly increased in the H/R group, along with increased production of reactive oxygen species (ROS) and iron ions. When the expression of miR-200a-3p was inhibited, iron ions and ROS levels decreased significantly. Western blotting showed that transferrin receptor (TFRC) and Acyl-coA synthetase long-chain family member 4 (ACSL4) levels were decreased and Glutathione peroxidase 4 (GPX4) expression was increased. Conclusions: These findings offer a novel perspective on I/R regulation, and the specific mechanisms underlying the actions of miR-200a-3p merit further investigation. |
| first_indexed | 2024-03-08T09:37:12Z |
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| institution | Directory Open Access Journal |
| issn | 2768-6701 |
| language | English |
| last_indexed | 2024-03-08T09:37:12Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Frontiers in Bioscience-Landmark |
| spelling | doaj.art-cc69a0107096487c8933c64c45e312b52024-01-30T07:44:34ZengIMR PressFrontiers in Bioscience-Landmark2768-67012024-01-012913810.31083/j.fbl2901038S2768-6701(23)01099-7High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion InjurySenjie Li0Dongqing Lv1Yan Lu2Yanwei Zhang3Yongping Jia4Department of Cardiology, First Hospital of Shanxi Medical University, 030001 Taiyuan, Shanxi, ChinaDepartment of Endocrinology, First Hospital of Shanxi Medical University, 030001 Taiyuan, Shanxi, ChinaDepartment of Cardiology, First Hospital of Shanxi Medical University, 030001 Taiyuan, Shanxi, ChinaDepartment of Cardiology, First Hospital of Shanxi Medical University, 030001 Taiyuan, Shanxi, ChinaDepartment of Cardiology, First Hospital of Shanxi Medical University, 030001 Taiyuan, Shanxi, ChinaBackground: microRNAs (miRNAs) are closely associated with the pathogenesis of various diseases, but the relationship between miRNAs and myocardial ischemia-reperfusion (I/R) injury remains unclear. Therefore, we aimed to explore the role and function of miRNAs and identify target genes regulating I/R. Methods: We established a hypoxia/reoxygenation (H/R) model to detect differentially expressed miRNAs using high-throughput sequencing in rat myocardial cells. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment were used to analyze the potential functions and signaling pathways of target genes. Results: We identified 113 differentially expressed miRNAs, comprising 76 and 37 upregulated and downregulated genes, respectively. Database predictions suggested that miR-200a-3p may act through the ferroptosis pathway, and we assessed the expression of miR-200a-3p, iron ions, and ferroptosis markers. The expression of miR-200a-3p significantly increased in the H/R group, along with increased production of reactive oxygen species (ROS) and iron ions. When the expression of miR-200a-3p was inhibited, iron ions and ROS levels decreased significantly. Western blotting showed that transferrin receptor (TFRC) and Acyl-coA synthetase long-chain family member 4 (ACSL4) levels were decreased and Glutathione peroxidase 4 (GPX4) expression was increased. Conclusions: These findings offer a novel perspective on I/R regulation, and the specific mechanisms underlying the actions of miR-200a-3p merit further investigation.https://www.imrpress.com/journal/FBL/29/1/10.31083/j.fbl2901038myocardial cellsmicrornaischemia-reperfusionhigh-throughput sequencingferroptosis |
| spellingShingle | Senjie Li Dongqing Lv Yan Lu Yanwei Zhang Yongping Jia High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury Frontiers in Bioscience-Landmark myocardial cells microrna ischemia-reperfusion high-throughput sequencing ferroptosis |
| title | High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury |
| title_full | High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury |
| title_fullStr | High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury |
| title_full_unstemmed | High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury |
| title_short | High-Throughput Sequencing to Investigate the Expression and Potential Role of Differentially Expressed microRNAs in Myocardial Cells after Ischemia-Reperfusion Injury |
| title_sort | high throughput sequencing to investigate the expression and potential role of differentially expressed micrornas in myocardial cells after ischemia reperfusion injury |
| topic | myocardial cells microrna ischemia-reperfusion high-throughput sequencing ferroptosis |
| url | https://www.imrpress.com/journal/FBL/29/1/10.31083/j.fbl2901038 |
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