Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche
The utility of immunomagnetic separation (IMS) for the recovery of Escherichia coli O157:H7 in milk creams was evaluated. The efficiency of different pre-enrichments broths previous to ISM was determined and different agars were analyzed (differential and/or selective) for plating of the inmunobeads...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedad Latinoamericana de Nutrición
|
| Series: | Archivos Latinoamericanos de Nutrición |
| Subjects: | |
| Online Access: | http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222006000300008&lng=en&tlng=en |
| _version_ | 1797965228582895616 |
|---|---|
| author | Tomás Rojas Ysvette Vásquez Doris Reyes Carlos Martínez Luís Medina |
| author_facet | Tomás Rojas Ysvette Vásquez Doris Reyes Carlos Martínez Luís Medina |
| author_sort | Tomás Rojas |
| collection | DOAJ |
| description | The utility of immunomagnetic separation (IMS) for the recovery of Escherichia coli O157:H7 in milk creams was evaluated. The efficiency of different pre-enrichments broths previous to ISM was determined and different agars were analyzed (differential and/or selective) for plating of the inmunobeads. Portions of 25 g from milk creams were inoculated with pathogen low levels (50, 10 and 1 cell/g) and then, were enriched in buffered peptone water with vancomycin (BPW-V), buffered peptone water with vancomycin and cefixime (BPW-VC) and triptone soya broth with vancomycin, cefixime and tellurite (TSB-VCT) being incubated 35ºC/18 hours. Then, IMS was applied and immunobeads were plated onto sorbitol MacConkey agar (SMA), sorbitol MacConkey agar with cefixime and tellurite (SMA-CT) and chromogenic agar with tellurite and cefixime (CRGA-TC). It was observed significant differences (p<0,05) in the recovery percentages according to the pre-enrichment broth used, being obtained the biggest recovery rates with the use of TSB-VCT for the three inocula levels. The recovery rates did not vary significantly (p<0,05) among the utilized agars, while the number of inoculated cells impacts the recovery percentages, being bigger as it increases the inocula. In conclusion, IMS is a sensitive method for the recovery of Escherichia coli O157:H7 in milk creams, recommending for isolation of this pathogen, the enrichment in TSB-VCT, application of IMS, and plating of immunobeads onto nonselective agar (SMA) and selective agar (SMA-TC or CRGA-TC). |
| first_indexed | 2024-04-11T01:56:42Z |
| format | Article |
| id | doaj.art-cc6a3cbfc3e74d7080cb205013bc67d9 |
| institution | Directory Open Access Journal |
| issn | 0004-0622 |
| language | English |
| last_indexed | 2024-04-11T01:56:42Z |
| publisher | Sociedad Latinoamericana de Nutrición |
| record_format | Article |
| series | Archivos Latinoamericanos de Nutrición |
| spelling | doaj.art-cc6a3cbfc3e74d7080cb205013bc67d92023-01-03T05:11:28ZengSociedad Latinoamericana de NutriciónArchivos Latinoamericanos de Nutrición0004-0622563257264S0004-06222006000300008Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de lecheTomás Rojas0Ysvette VásquezDoris ReyesCarlos MartínezLuís MedinaUnidad de Microbiologia AmbientalThe utility of immunomagnetic separation (IMS) for the recovery of Escherichia coli O157:H7 in milk creams was evaluated. The efficiency of different pre-enrichments broths previous to ISM was determined and different agars were analyzed (differential and/or selective) for plating of the inmunobeads. Portions of 25 g from milk creams were inoculated with pathogen low levels (50, 10 and 1 cell/g) and then, were enriched in buffered peptone water with vancomycin (BPW-V), buffered peptone water with vancomycin and cefixime (BPW-VC) and triptone soya broth with vancomycin, cefixime and tellurite (TSB-VCT) being incubated 35ºC/18 hours. Then, IMS was applied and immunobeads were plated onto sorbitol MacConkey agar (SMA), sorbitol MacConkey agar with cefixime and tellurite (SMA-CT) and chromogenic agar with tellurite and cefixime (CRGA-TC). It was observed significant differences (p<0,05) in the recovery percentages according to the pre-enrichment broth used, being obtained the biggest recovery rates with the use of TSB-VCT for the three inocula levels. The recovery rates did not vary significantly (p<0,05) among the utilized agars, while the number of inoculated cells impacts the recovery percentages, being bigger as it increases the inocula. In conclusion, IMS is a sensitive method for the recovery of Escherichia coli O157:H7 in milk creams, recommending for isolation of this pathogen, the enrichment in TSB-VCT, application of IMS, and plating of immunobeads onto nonselective agar (SMA) and selective agar (SMA-TC or CRGA-TC).http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222006000300008&lng=en&tlng=enescherichia coli o157:h7inmunoseparación magnéticaproductos lácteosescherichia coli o157:h7immunomagnetic separationdairy products |
| spellingShingle | Tomás Rojas Ysvette Vásquez Doris Reyes Carlos Martínez Luís Medina Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche Archivos Latinoamericanos de Nutrición escherichia coli o157:h7 inmunoseparación magnética productos lácteos escherichia coli o157:h7 immunomagnetic separation dairy products |
| title | Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche |
| title_full | Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche |
| title_fullStr | Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche |
| title_full_unstemmed | Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche |
| title_short | Evaluación de la técnica de inmunoseparación magnética para recuperación de Escherichia coli O157:H7 en cremas de leche |
| title_sort | evaluacion de la tecnica de inmunoseparacion magnetica para recuperacion de escherichia coli o157 h7 en cremas de leche |
| topic | escherichia coli o157:h7 inmunoseparación magnética productos lácteos escherichia coli o157:h7 immunomagnetic separation dairy products |
| url | http://www.scielo.org.ve/scielo.php?script=sci_arttext&pid=S0004-06222006000300008&lng=en&tlng=en |
| work_keys_str_mv | AT tomasrojas evaluaciondelatecnicadeinmunoseparacionmagneticapararecuperaciondeescherichiacolio157h7encremasdeleche AT ysvettevasquez evaluaciondelatecnicadeinmunoseparacionmagneticapararecuperaciondeescherichiacolio157h7encremasdeleche AT dorisreyes evaluaciondelatecnicadeinmunoseparacionmagneticapararecuperaciondeescherichiacolio157h7encremasdeleche AT carlosmartinez evaluaciondelatecnicadeinmunoseparacionmagneticapararecuperaciondeescherichiacolio157h7encremasdeleche AT luismedina evaluaciondelatecnicadeinmunoseparacionmagneticapararecuperaciondeescherichiacolio157h7encremasdeleche |