Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response
Petunias are important ornamentals with the capacity for cold acclimation. So far, there is limited information concerning gene regulation and signaling pathways related to the cold stress response in petunias. A custom-designed petunia microarray representing 24816 genes was used to perform transcr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00118/full |
| _version_ | 1818584887773364224 |
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| author | Bei eLi Luyun eNing Junwei eZhang Manzhu eBao Wei eZhang |
| author_facet | Bei eLi Luyun eNing Junwei eZhang Manzhu eBao Wei eZhang |
| author_sort | Bei eLi |
| collection | DOAJ |
| description | Petunias are important ornamentals with the capacity for cold acclimation. So far, there is limited information concerning gene regulation and signaling pathways related to the cold stress response in petunias. A custom-designed petunia microarray representing 24816 genes was used to perform transcriptome profiling in petunia seedlings subjected to cold at 2°C for 0.5 h, 2 h, 24 h and 5 d. A total of 2071 transcripts displayed differential expression patterns under cold stress, of which 1149 were up-regulated and 922 were down-regulated. Gene ontology enrichment analysis demarcated related biological processes, suggesting a possible link between flavonoid metabolism and plant adaptation to low temperatures. Many novel stress-responsive regulators were revealed, suggesting that diverse regulatory pathways may exist in petunias in addition to the well-characterized CBF pathway. The expression changes of selected genes under cold and other abiotic stress conditions were confirmed by real-time RT-PCR. Furthermore, weighted gene co-expression network analysis divided the petunia genes on the array into 65 modules that showed high co-expression and identified stress-specific hub genes with high connectivity. Our identification of these transcriptional responses and groups of differentially expressed regulators will facilitate the functional dissection of the molecular mechanism in petunias responding to environment stresses and extend our ability to improve cold tolerance in plants. |
| first_indexed | 2024-12-16T08:28:19Z |
| format | Article |
| id | doaj.art-cc6c74d620894939b79e2781642e5cd1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T08:28:19Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-cc6c74d620894939b79e2781642e5cd12022-12-21T22:37:57ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-03-01610.3389/fpls.2015.00118129208Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress responseBei eLi0Luyun eNing1Junwei eZhang2Manzhu eBao3Wei eZhang4Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences,Huazhong Agricultural UniversityPetunias are important ornamentals with the capacity for cold acclimation. So far, there is limited information concerning gene regulation and signaling pathways related to the cold stress response in petunias. A custom-designed petunia microarray representing 24816 genes was used to perform transcriptome profiling in petunia seedlings subjected to cold at 2°C for 0.5 h, 2 h, 24 h and 5 d. A total of 2071 transcripts displayed differential expression patterns under cold stress, of which 1149 were up-regulated and 922 were down-regulated. Gene ontology enrichment analysis demarcated related biological processes, suggesting a possible link between flavonoid metabolism and plant adaptation to low temperatures. Many novel stress-responsive regulators were revealed, suggesting that diverse regulatory pathways may exist in petunias in addition to the well-characterized CBF pathway. The expression changes of selected genes under cold and other abiotic stress conditions were confirmed by real-time RT-PCR. Furthermore, weighted gene co-expression network analysis divided the petunia genes on the array into 65 modules that showed high co-expression and identified stress-specific hub genes with high connectivity. Our identification of these transcriptional responses and groups of differentially expressed regulators will facilitate the functional dissection of the molecular mechanism in petunias responding to environment stresses and extend our ability to improve cold tolerance in plants.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00118/fullGene ExpressionMicroarraytranscriptional profilingabiotic stressPetunia hybridacold-responsive regulator |
| spellingShingle | Bei eLi Luyun eNing Junwei eZhang Manzhu eBao Wei eZhang Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response Frontiers in Plant Science Gene Expression Microarray transcriptional profiling abiotic stress Petunia hybrida cold-responsive regulator |
| title | Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response |
| title_full | Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response |
| title_fullStr | Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response |
| title_full_unstemmed | Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response |
| title_short | Transcriptional profiling of Petunia seedlings reveals candidate regulators of the cold stress response |
| title_sort | transcriptional profiling of petunia seedlings reveals candidate regulators of the cold stress response |
| topic | Gene Expression Microarray transcriptional profiling abiotic stress Petunia hybrida cold-responsive regulator |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00118/full |
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