The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment
Abstract The interactions between environmental factors and bacterial community shift in solid-phase denitrification are crucial for optimum operation of a reactor and to achieve maximum treatment efficiency. In this study, Illumina high-throughput sequencing was applied to reveal the effects of dif...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-06-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-017-0412-3 |
| _version_ | 1811263647949783040 |
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| author | Ya-Le Deng Yun-Jie Ruan Song-Ming Zhu Xi-Shan Guo Zhi-Ying Han Zhang-Ying Ye Gang Liu Ming-Ming Shi |
| author_facet | Ya-Le Deng Yun-Jie Ruan Song-Ming Zhu Xi-Shan Guo Zhi-Ying Han Zhang-Ying Ye Gang Liu Ming-Ming Shi |
| author_sort | Ya-Le Deng |
| collection | DOAJ |
| description | Abstract The interactions between environmental factors and bacterial community shift in solid-phase denitrification are crucial for optimum operation of a reactor and to achieve maximum treatment efficiency. In this study, Illumina high-throughput sequencing was applied to reveal the effects of different operational conditions on bacterial community distribution of three continuous operated poly(butylene succinate) biological denitrification reactors used for recirculating aquaculture system (RAS) wastewater treatment. The results indicated that salinity decreased OTU numbers and diversity while dissolved oxygen (DO) had no obvious influence on OTU numbers. Significant microbial community composition differences were observed among and between three denitrification reactors under varied operation conditions. This result was also demonstrated by cluster analysis (CA) and detrended correspondence analysis (DCA). Hierarchical clustering and redundancy analysis (RDA) was performed to test the relationship between environmental factors and bacterial community compositions and result indicated that salinity, DO and hydraulic retention time (HRT) were the three key factors in microbial community formation. Besides, Simplicispira was detected under all operational conditions, which worth drawing more attention for nitrate removal. Moreover, the abundance of nosZ gene and 16S rRNA were analyzed by real-time PCR, which suggested that salinity decreased the proportion of denitrifiers among whole bacterial community while DO had little influence on marine reactors. This study provides an overview of microbial community shift dynamics in solid-phase denitrification reactors when operation parameters changed and proved the feasibility to apply interval aeration for denitrification process based on microbial level, which may shed light on improving the performance of RAS treatment units. |
| first_indexed | 2024-04-12T19:49:08Z |
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| id | doaj.art-cc6dc462f27640cfbbd9cf61fd75242a |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-12T19:49:08Z |
| publishDate | 2017-06-01 |
| publisher | SpringerOpen |
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| series | AMB Express |
| spelling | doaj.art-cc6dc462f27640cfbbd9cf61fd75242a2022-12-22T03:18:53ZengSpringerOpenAMB Express2191-08552017-06-017111110.1186/s13568-017-0412-3The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatmentYa-Le Deng0Yun-Jie Ruan1Song-Ming Zhu2Xi-Shan Guo3Zhi-Ying Han4Zhang-Ying Ye5Gang Liu6Ming-Ming Shi7Institute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityInstitute of Agricultural Bio-Environmental Engineering, College of Bio-systems Engineering and Food Science, Zhejiang UniversityAbstract The interactions between environmental factors and bacterial community shift in solid-phase denitrification are crucial for optimum operation of a reactor and to achieve maximum treatment efficiency. In this study, Illumina high-throughput sequencing was applied to reveal the effects of different operational conditions on bacterial community distribution of three continuous operated poly(butylene succinate) biological denitrification reactors used for recirculating aquaculture system (RAS) wastewater treatment. The results indicated that salinity decreased OTU numbers and diversity while dissolved oxygen (DO) had no obvious influence on OTU numbers. Significant microbial community composition differences were observed among and between three denitrification reactors under varied operation conditions. This result was also demonstrated by cluster analysis (CA) and detrended correspondence analysis (DCA). Hierarchical clustering and redundancy analysis (RDA) was performed to test the relationship between environmental factors and bacterial community compositions and result indicated that salinity, DO and hydraulic retention time (HRT) were the three key factors in microbial community formation. Besides, Simplicispira was detected under all operational conditions, which worth drawing more attention for nitrate removal. Moreover, the abundance of nosZ gene and 16S rRNA were analyzed by real-time PCR, which suggested that salinity decreased the proportion of denitrifiers among whole bacterial community while DO had little influence on marine reactors. This study provides an overview of microbial community shift dynamics in solid-phase denitrification reactors when operation parameters changed and proved the feasibility to apply interval aeration for denitrification process based on microbial level, which may shed light on improving the performance of RAS treatment units.http://link.springer.com/article/10.1186/s13568-017-0412-3Recirculating aquaculture systemBiological denitrificationSalinityDissolved oxygenMicrobial communityReal-time PCR |
| spellingShingle | Ya-Le Deng Yun-Jie Ruan Song-Ming Zhu Xi-Shan Guo Zhi-Ying Han Zhang-Ying Ye Gang Liu Ming-Ming Shi The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment AMB Express Recirculating aquaculture system Biological denitrification Salinity Dissolved oxygen Microbial community Real-time PCR |
| title | The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment |
| title_full | The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment |
| title_fullStr | The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment |
| title_full_unstemmed | The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment |
| title_short | The impact of DO and salinity on microbial community in poly(butylene succinate) denitrification reactors for recirculating aquaculture system wastewater treatment |
| title_sort | impact of do and salinity on microbial community in poly butylene succinate denitrification reactors for recirculating aquaculture system wastewater treatment |
| topic | Recirculating aquaculture system Biological denitrification Salinity Dissolved oxygen Microbial community Real-time PCR |
| url | http://link.springer.com/article/10.1186/s13568-017-0412-3 |
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