Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea
Background: Escherichia coli strains include both commensal and virulent clones distributed in different phylogenetic groups. Antimicrobial resistance is an increasingly serious public health threat at the global level and integrons are important mobile genetic elements involved in resistance dissem...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-05-01
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| Series: | Journal of Infection and Public Health |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034119303648 |
| _version_ | 1811286637467926528 |
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| author | Yesmi Patricia Ahumada-Santos María Elena Báez-Flores Sylvia Páz Díaz-Camacho Magdalena de Jesús Uribe-Beltrán Carlos Alberto Eslava-Campos Jesús Ricardo Parra-Unda Francisco Delgado-Vargas |
| author_facet | Yesmi Patricia Ahumada-Santos María Elena Báez-Flores Sylvia Páz Díaz-Camacho Magdalena de Jesús Uribe-Beltrán Carlos Alberto Eslava-Campos Jesús Ricardo Parra-Unda Francisco Delgado-Vargas |
| author_sort | Yesmi Patricia Ahumada-Santos |
| collection | DOAJ |
| description | Background: Escherichia coli strains include both commensal and virulent clones distributed in different phylogenetic groups. Antimicrobial resistance is an increasingly serious public health threat at the global level and integrons are important mobile genetic elements involved in resistance dissemination. This paper aims to determine the phylogenetic groups and presence of class 1 (intl1) and 2 (intl2) integrons in E. coli clinical isolates from children with diarrhoea, and to associate these characteristics with their antimicrobial resistance. Methods: Phylogeny and presence of integrons (intl1 and intl2) were analysed by PCR and amplicon sequencing in 70 E. coli isolates from children with and without diarrhoea (35 of each group) from Sinaloa, Mexico; these variables were analysed for correlation with the antimicrobial resistance profile of the isolates. Results: The most frequent phylogroups were A (42.9%) and B2 (15.7%). The E. coli isolates from children with diarrhoea were distributed in all phylogroups; while strains from children without diarrhoea were absent from phylogroups C, E, and clade I. The 17.1% of the isolates carried integrons (15.7% intI1 and 1.4% intI2); 28.6% of the isolates from children with diarrhoea showed the class 1 integron. Strains of phylogroup A showed the highest frequency of integrons (33.3%). The association of multidrug resistance and the presence of integrons was identified in 58.3% of strains isolated from children with diarrhoea included in phylogroups A and B2. The sequence analysis of intl1 and intl2 showed silent point mutations and similarities with plasmids of some APEC and AIEC strains. Conclusion: Commensal E. coli strains are potential disseminators of antimicrobial resistance, and the improvement in the use of antimicrobials to treat childhood diarrhoea is essential for the control of such resistance. Keywords: Escherichia coli, Phylogeny, Integrons, Antimicrobial resistance, Diarrhoea |
| first_indexed | 2024-04-13T03:04:33Z |
| format | Article |
| id | doaj.art-cc6f28c301de4396bb4881e72ba3f23c |
| institution | Directory Open Access Journal |
| issn | 1876-0341 |
| language | English |
| last_indexed | 2024-04-13T03:04:33Z |
| publishDate | 2020-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj.art-cc6f28c301de4396bb4881e72ba3f23c2022-12-22T03:05:19ZengElsevierJournal of Infection and Public Health1876-03412020-05-01135767772Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoeaYesmi Patricia Ahumada-Santos0María Elena Báez-Flores1Sylvia Páz Díaz-Camacho2Magdalena de Jesús Uribe-Beltrán3Carlos Alberto Eslava-Campos4Jesús Ricardo Parra-Unda5Francisco Delgado-Vargas6School of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan, Sinaloa, MexicoSchool of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan, Sinaloa, MexicoResearch Unit in Environment and Health, Autonomous University of Occident, Culiacan, Sinaloa, MexicoSchool of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan, Sinaloa, MexicoSchool of Medicine, National Autonomous University of Mexico, Ciudad Universitaria, Coyoacan, 04510, Ciudad de Mexico, Mexico; Laboratory of Bacterial Pathogenicity, Hemato Oncology and Research Unit, Hospital Infantil de Mexico Federico Gomez 06720, Ciudad de Mexico, MexicoSchool of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan, Sinaloa, MexicoSchool of Chemical and Biological Sciences, Autonomous University of Sinaloa, Ciudad Universitaria, Culiacan, Sinaloa, Mexico; Corresponding author.Background: Escherichia coli strains include both commensal and virulent clones distributed in different phylogenetic groups. Antimicrobial resistance is an increasingly serious public health threat at the global level and integrons are important mobile genetic elements involved in resistance dissemination. This paper aims to determine the phylogenetic groups and presence of class 1 (intl1) and 2 (intl2) integrons in E. coli clinical isolates from children with diarrhoea, and to associate these characteristics with their antimicrobial resistance. Methods: Phylogeny and presence of integrons (intl1 and intl2) were analysed by PCR and amplicon sequencing in 70 E. coli isolates from children with and without diarrhoea (35 of each group) from Sinaloa, Mexico; these variables were analysed for correlation with the antimicrobial resistance profile of the isolates. Results: The most frequent phylogroups were A (42.9%) and B2 (15.7%). The E. coli isolates from children with diarrhoea were distributed in all phylogroups; while strains from children without diarrhoea were absent from phylogroups C, E, and clade I. The 17.1% of the isolates carried integrons (15.7% intI1 and 1.4% intI2); 28.6% of the isolates from children with diarrhoea showed the class 1 integron. Strains of phylogroup A showed the highest frequency of integrons (33.3%). The association of multidrug resistance and the presence of integrons was identified in 58.3% of strains isolated from children with diarrhoea included in phylogroups A and B2. The sequence analysis of intl1 and intl2 showed silent point mutations and similarities with plasmids of some APEC and AIEC strains. Conclusion: Commensal E. coli strains are potential disseminators of antimicrobial resistance, and the improvement in the use of antimicrobials to treat childhood diarrhoea is essential for the control of such resistance. Keywords: Escherichia coli, Phylogeny, Integrons, Antimicrobial resistance, Diarrhoeahttp://www.sciencedirect.com/science/article/pii/S1876034119303648 |
| spellingShingle | Yesmi Patricia Ahumada-Santos María Elena Báez-Flores Sylvia Páz Díaz-Camacho Magdalena de Jesús Uribe-Beltrán Carlos Alberto Eslava-Campos Jesús Ricardo Parra-Unda Francisco Delgado-Vargas Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea Journal of Infection and Public Health |
| title | Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea |
| title_full | Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea |
| title_fullStr | Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea |
| title_full_unstemmed | Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea |
| title_short | Association of phylogenetic distribution and presence of integrons with multidrug resistance in Escherichia coli clinical isolates from children with diarrhoea |
| title_sort | association of phylogenetic distribution and presence of integrons with multidrug resistance in escherichia coli clinical isolates from children with diarrhoea |
| url | http://www.sciencedirect.com/science/article/pii/S1876034119303648 |
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