Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol
ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatchi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zootecnia
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| Series: | Revista Brasileira de Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155&lng=en&tlng=en |
| _version_ | 1818235558915211264 |
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| author | Gilmara Junqueira Machado Luis David Solis Murgas Priscila Cotta Palhares Isadora de Lima Assis Tássia Flávia Dias Castro |
| author_facet | Gilmara Junqueira Machado Luis David Solis Murgas Priscila Cotta Palhares Isadora de Lima Assis Tássia Flávia Dias Castro |
| author_sort | Gilmara Junqueira Machado |
| collection | DOAJ |
| description | ABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae. |
| first_indexed | 2024-12-12T11:55:53Z |
| format | Article |
| id | doaj.art-cc72fd1a4cd648c4bdb18be02d295529 |
| institution | Directory Open Access Journal |
| issn | 1806-9290 |
| language | English |
| last_indexed | 2024-12-12T11:55:53Z |
| publisher | Sociedade Brasileira de Zootecnia |
| record_format | Article |
| series | Revista Brasileira de Zootecnia |
| spelling | doaj.art-cc72fd1a4cd648c4bdb18be02d2955292022-12-22T00:25:12ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92904810.1590/rbz4820160377S1516-35982019000100155Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanolGilmara Junqueira MachadoLuis David Solis MurgasPriscila Cotta PalharesIsadora de Lima AssisTássia Flávia Dias CastroABSTRACT This study aimed to evaluate the effect of using different concentrations (5, 7.5, 10, and 12.5%) of two permeating cryoprotectants, dimethyl sulphoxide (DMSO) and methanol, on Prochilodus lineatus embryos while being subjected to cooling for 2, 4, 6, and 8 h at 4 °C. We analyzed the hatching rate, viable larvae, and counts of hatched and spoiled eggs and those that did not complete their development after cooling. We observed a significant interaction among variables, permeable cryoprotectant concentration, and cooling time, having the increase of these factors caused a reduction in hatching rate. The curimba embryos showed a higher sensitivity to cold at a temperature of 4 °C for 6 and 8 h, directly influencing the production of viable larvae. We did not observe a significant difference in the variables analyzed when comparing the cryoprotectant solutions containing DMSO, methanol, and solutions containing only water and sucrose (0.5M). An increase in the concentration of cryoprotectant and cooling time promotes a reduction in the hatching rate and in the percentage of viable curimba larvae.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155&lng=en&tlng=enaquaculturebiotechnologycryopreservationeggsfishlarvae |
| spellingShingle | Gilmara Junqueira Machado Luis David Solis Murgas Priscila Cotta Palhares Isadora de Lima Assis Tássia Flávia Dias Castro Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol Revista Brasileira de Zootecnia aquaculture biotechnology cryopreservation eggs fish larvae |
| title | Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
| title_full | Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
| title_fullStr | Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
| title_full_unstemmed | Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
| title_short | Cooling of curimba (Prochilodus lineatus) embryos using different concentrations of dimethyl sulphoxide and methanol |
| title_sort | cooling of curimba prochilodus lineatus embryos using different concentrations of dimethyl sulphoxide and methanol |
| topic | aquaculture biotechnology cryopreservation eggs fish larvae |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100155&lng=en&tlng=en |
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