Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt
Staphylococcal infections in poultry are taking an increasing significance and both coagulase positive Staphylococci (CoPS) and coagulase negative Staphylococci (CoNS) can cause infections in poultry. On the other hand, methicillin resistance Staphylococci (MRS) is a global problem currently and M...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
South Valley University
2021-10-01
|
| Series: | SVU-International Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://svu.journals.ekb.eg/article_200549.html |
| _version_ | 1818673314182201344 |
|---|---|
| author | Waleed Younis Mahmoud Sabra Haitham Helmy Sayed |
| author_facet | Waleed Younis Mahmoud Sabra Haitham Helmy Sayed |
| author_sort | Waleed Younis |
| collection | DOAJ |
| description | Staphylococcal infections in poultry are taking an increasing significance and both coagulase positive
Staphylococci (CoPS) and coagulase negative Staphylococci (CoNS) can cause infections in poultry. On the
other hand, methicillin resistance Staphylococci (MRS) is a global problem currently and MRS are often has
multidrug resistance (MDR) against variety of the other antibiotics. This study was performed to investigate
incidence of CoPS and CoNS and their characteristics among Japanese quails and broiler chickens in some
farms at Qena Governorate Therefore, 80 Japanese quail samples of 3-4 weeks old and 70 broiler chicken
samples of 21-25 days old were collected from different localities in Qena Governorate during the period
from October 2020 to March 2021 for analysis. Bacterial isolates were identified as Staphylococci through
phenotypic characteristics and PCR assay target 16s rRNA gene of Staphylococci, additionally CoPS were
divided into Staphylococcus aureus(S. aureus) and other coagulase positive Staphylococci (OCoPS) by PCR
assay target nuc gene of S. aureus. Bacteriologically, 11 Staphylococcus isolates were isolated from Japanese
quail samples with percentage of (13.75%) and they identified as S. aureus (n=2), OCoPS (n=2) and CoNS
(n=7) while 5 CoNS isolates were isolated and identified from broiler chicken samples with percentage of
(7.14%). Examination of Staphylococcus isolates for their ability to form biofilm by Microtiter plate (MTP)
and Congo Red Agar (CRA) tests revealed that all isolates were biofilm producer but with varied grades of
biofilm production and the correlation rate between MTP and CRA tests for detection of biofilm-producing
Staphylococci were 93.75%. Antimicrobial susceptibility testing of Staphylococcus isolates revealed that all
the isolates were resistant to ampicillin, oxacillin, cefazolin, cefotaxime, vancomycin, erythromycin and
clindamycin and they had antibiotic sensitivity to ciprofloxacin (87.5%) followed by gentamicin (75.0%),
amoxicillin/clavulanic acid (62.5%), oxytetracycline (25%) and chloramphenicol (12.5%). On the other
hand, screening Staphylococcus isolates by PCR for presence of mecA, blaz and vanA resistance genes
revealed that (81.25%), (56.25%) and (31.25%) of the isolates harbor these genes respectively and each MRS
harbor one gene of mecA and blaz at least. The present study demonstrates that Japanese quails and chickens
harbor multidrug-resistant bacteria that could be transmitted to human |
| first_indexed | 2024-12-17T07:53:49Z |
| format | Article |
| id | doaj.art-cc74f1a761eb49fab590ae07f6a5e9c4 |
| institution | Directory Open Access Journal |
| issn | 2535-1826 2535-1877 |
| language | English |
| last_indexed | 2024-12-17T07:53:49Z |
| publishDate | 2021-10-01 |
| publisher | South Valley University |
| record_format | Article |
| series | SVU-International Journal of Veterinary Sciences |
| spelling | doaj.art-cc74f1a761eb49fab590ae07f6a5e9c42022-12-21T21:57:46ZengSouth Valley UniversitySVU-International Journal of Veterinary Sciences2535-18262535-18772021-10-014411510.21608/SVU.2021.92987.1146Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, EgyptWaleed Younis0 Mahmoud Sabra1Haitham Helmy Sayed2microbiology department , faculty of veterinary medicine, south valley universityDepartment of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, EgyptDepartment of Microbiology, Faculty of Veterinary Medicine, Sohag University, Sohag, 82524, Egypt.Staphylococcal infections in poultry are taking an increasing significance and both coagulase positive Staphylococci (CoPS) and coagulase negative Staphylococci (CoNS) can cause infections in poultry. On the other hand, methicillin resistance Staphylococci (MRS) is a global problem currently and MRS are often has multidrug resistance (MDR) against variety of the other antibiotics. This study was performed to investigate incidence of CoPS and CoNS and their characteristics among Japanese quails and broiler chickens in some farms at Qena Governorate Therefore, 80 Japanese quail samples of 3-4 weeks old and 70 broiler chicken samples of 21-25 days old were collected from different localities in Qena Governorate during the period from October 2020 to March 2021 for analysis. Bacterial isolates were identified as Staphylococci through phenotypic characteristics and PCR assay target 16s rRNA gene of Staphylococci, additionally CoPS were divided into Staphylococcus aureus(S. aureus) and other coagulase positive Staphylococci (OCoPS) by PCR assay target nuc gene of S. aureus. Bacteriologically, 11 Staphylococcus isolates were isolated from Japanese quail samples with percentage of (13.75%) and they identified as S. aureus (n=2), OCoPS (n=2) and CoNS (n=7) while 5 CoNS isolates were isolated and identified from broiler chicken samples with percentage of (7.14%). Examination of Staphylococcus isolates for their ability to form biofilm by Microtiter plate (MTP) and Congo Red Agar (CRA) tests revealed that all isolates were biofilm producer but with varied grades of biofilm production and the correlation rate between MTP and CRA tests for detection of biofilm-producing Staphylococci were 93.75%. Antimicrobial susceptibility testing of Staphylococcus isolates revealed that all the isolates were resistant to ampicillin, oxacillin, cefazolin, cefotaxime, vancomycin, erythromycin and clindamycin and they had antibiotic sensitivity to ciprofloxacin (87.5%) followed by gentamicin (75.0%), amoxicillin/clavulanic acid (62.5%), oxytetracycline (25%) and chloramphenicol (12.5%). On the other hand, screening Staphylococcus isolates by PCR for presence of mecA, blaz and vanA resistance genes revealed that (81.25%), (56.25%) and (31.25%) of the isolates harbor these genes respectively and each MRS harbor one gene of mecA and blaz at least. The present study demonstrates that Japanese quails and chickens harbor multidrug-resistant bacteria that could be transmitted to humanhttps://svu.journals.ekb.eg/article_200549.htmlcharacterizationcoagulase positivecoagulase negativestaphylococci |
| spellingShingle | Waleed Younis Mahmoud Sabra Haitham Helmy Sayed Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt SVU-International Journal of Veterinary Sciences characterization coagulase positive coagulase negative staphylococci |
| title | Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt |
| title_full | Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt |
| title_fullStr | Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt |
| title_full_unstemmed | Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt |
| title_short | Occurrence and characterization of coagulase positive and negative Staphylococci isolated from Japanese quails and broiler chickens at Qena Governorate, Egypt |
| title_sort | occurrence and characterization of coagulase positive and negative staphylococci isolated from japanese quails and broiler chickens at qena governorate egypt |
| topic | characterization coagulase positive coagulase negative staphylococci |
| url | https://svu.journals.ekb.eg/article_200549.html |
| work_keys_str_mv | AT waleedyounis occurrenceandcharacterizationofcoagulasepositiveandnegativestaphylococciisolatedfromjapanesequailsandbroilerchickensatqenagovernorateegypt AT mahmoudsabra occurrenceandcharacterizationofcoagulasepositiveandnegativestaphylococciisolatedfromjapanesequailsandbroilerchickensatqenagovernorateegypt AT haithamhelmysayed occurrenceandcharacterizationofcoagulasepositiveandnegativestaphylococciisolatedfromjapanesequailsandbroilerchickensatqenagovernorateegypt |