Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i>
<i>Candida tropicalis</i>, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant <i>C</i><i>. tropicalis</i> X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual incr...
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MDPI AG
2022-01-01
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| Online Access: | https://www.mdpi.com/1996-1073/15/2/561 |
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| author | Koudkeo Phommachan Chansom Keo-oudone Mochamad Nurcholis Nookhao Vongvilaisak Mingkhuan Chanhming Vanhnavong Savanhnaly Somchanh Bounphanmy Minenosuke Matsutani Tomoyuki Kosaka Savitree Limtong Mamoru Yamada |
| author_facet | Koudkeo Phommachan Chansom Keo-oudone Mochamad Nurcholis Nookhao Vongvilaisak Mingkhuan Chanhming Vanhnavong Savanhnaly Somchanh Bounphanmy Minenosuke Matsutani Tomoyuki Kosaka Savitree Limtong Mamoru Yamada |
| author_sort | Koudkeo Phommachan |
| collection | DOAJ |
| description | <i>Candida tropicalis</i>, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant <i>C</i><i>. tropicalis</i> X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also up-regulated. From these findings, it can be inferred that the up-regulation of genes, including the gene for a glucose transporter, is responsible for the phenotype of the adaptive strain. This study revealed part of the mechanisms of fermentability at high glucose concentrations in <i>C. tropicalis</i> and the results of this study suggest that RLCGT is an effective procedure for improving multistress tolerance. |
| first_indexed | 2024-03-10T01:32:59Z |
| format | Article |
| id | doaj.art-cc782dfca5bc476f96e7d4759e06bbfc |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-03-10T01:32:59Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-cc782dfca5bc476f96e7d4759e06bbfc2023-11-23T13:38:18ZengMDPI AGEnergies1996-10732022-01-0115256110.3390/en15020561Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i>Koudkeo Phommachan0Chansom Keo-oudone1Mochamad Nurcholis2Nookhao Vongvilaisak3Mingkhuan Chanhming4Vanhnavong Savanhnaly5Somchanh Bounphanmy6Minenosuke Matsutani7Tomoyuki Kosaka8Savitree Limtong9Mamoru Yamada10Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, LaosDepartment of Food Science and Technology, Faculty of Agricultural Technology, Brawijaya University, Malang 65145, IndonesiaDepartment of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, LaosDepartment of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, LaosDepartment of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, LaosDepartment of Biology, Faculty of Natural Science, National University of Laos, Vientiane 7322, LaosDepartment of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, JapanGraduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandGraduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan<i>Candida tropicalis</i>, a xylose-fermenting yeast, has the potential for converting cellulosic biomass to ethanol. Thermotolerant <i>C</i><i>. tropicalis</i> X-17, which was isolated in Laos, was subjected to repetitive long-term cultivation with a gradual increase in temperature (RLCGT) in the presence of a high concentration of glucose, which exposed cells to various stresses in addition to the high concentration of glucose and high temperatures. The resultant adapted strain demonstrated increased tolerance to ethanol, furfural and hydroxymethylfurfural at high temperatures and displayed improvement in fermentation ability at high glucose concentrations and xylose-fermenting ability. Transcriptome analysis revealed the up-regulation of a gene for a glucose transporter of the major facilitator superfamily and genes for stress response and cell wall proteins. Additionally, hydropathy analysis revealed that three genes for putative membrane proteins with multiple membrane-spanning segments were also up-regulated. From these findings, it can be inferred that the up-regulation of genes, including the gene for a glucose transporter, is responsible for the phenotype of the adaptive strain. This study revealed part of the mechanisms of fermentability at high glucose concentrations in <i>C. tropicalis</i> and the results of this study suggest that RLCGT is an effective procedure for improving multistress tolerance.https://www.mdpi.com/1996-1073/15/2/561<i>Candida tropicalis</i>fermenting yeastadaptive laboratory evolutionfermentability at high glucose concentrationsmultistress tolerance |
| spellingShingle | Koudkeo Phommachan Chansom Keo-oudone Mochamad Nurcholis Nookhao Vongvilaisak Mingkhuan Chanhming Vanhnavong Savanhnaly Somchanh Bounphanmy Minenosuke Matsutani Tomoyuki Kosaka Savitree Limtong Mamoru Yamada Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> Energies <i>Candida tropicalis</i> fermenting yeast adaptive laboratory evolution fermentability at high glucose concentrations multistress tolerance |
| title | Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> |
| title_full | Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> |
| title_fullStr | Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> |
| title_full_unstemmed | Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> |
| title_short | Adaptive Laboratory Evolution for Multistress Tolerance, including Fermentability at High Glucose Concentrations in Thermotolerant <i>Candida tropicalis</i> |
| title_sort | adaptive laboratory evolution for multistress tolerance including fermentability at high glucose concentrations in thermotolerant i candida tropicalis i |
| topic | <i>Candida tropicalis</i> fermenting yeast adaptive laboratory evolution fermentability at high glucose concentrations multistress tolerance |
| url | https://www.mdpi.com/1996-1073/15/2/561 |
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