Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions
This study investigated the effects of L-glutamine (Gln) supplementation on growth performance, physiological traits, heat shock proteins (HSPs), and gene expression related to muscle and adipose tissue development in Hanwoo steers under heat stress (HS) conditions. Eight Hanwoo steers (initial body...
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2022-11-01
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| Series: | Journal of Animal Science and Technology |
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| Online Access: | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e80 |
| _version_ | 1811298753079934976 |
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| author | Yves Kamali Yong Ho Jo Won-Seob Kim Jalil Ghassemi Nejad Jae-Sung Lee Hong-Gu Lee |
| author_facet | Yves Kamali Yong Ho Jo Won-Seob Kim Jalil Ghassemi Nejad Jae-Sung Lee Hong-Gu Lee |
| author_sort | Yves Kamali |
| collection | DOAJ |
| description | This study investigated the effects of L-glutamine (Gln) supplementation on growth performance, physiological traits, heat shock proteins (HSPs), and gene expression related to muscle and adipose tissue development in Hanwoo steers under heat stress (HS) conditions. Eight Hanwoo steers (initial body weight [BW] 570.7 ± 43.6 kg, months of age 22.3 ± 0.88) were randomly separated into two groups, control and treatment, and supplied with the concentration (1.5% of BW kg/day/head) and rice straw (1.5 kg/day/head). The treatment group were fed the Gln supplementation (0.5% of concentration, as-fed basis) once a day at 08:00 h. Blood samples for the assessment of haematological and biochemical parameters and the separation of peripheral blood mononuclear cells (PBMCs) were collected four times, at 0, 3, 6, and 10 weeks of the experiment. Feed intake was measured daily. BW to analyze growth performance and hair follicle collection to analyze the expression of HSPs were executed four times at 0, 3, 6, and 10 weeks. To analyze gene expression, longissimus dorsi muscle samples were collected by biopsy at the end of the study. As a result, growing performance, including final BW, average daily gain, and gain-to-feed ratio, were not different between the two groups. Leukocytes including lymphocytes and granulocytes, tended to increase in the Gln supplementation group (p = 0.058). There were also no differences in biochemical parameters shown between the two groups, except total protein and albumin, both of which were lower in the Gln supplementation group (p < 0.05). Gene expressions related to muscle and adipose tissue development were not different between the two groups. As temperature–humidity index (THI) increased, HSP70 and HSP90 expression in the hair follicle showed a high correlation. HSP90 in the hair follicle was decreased in the treatment group compared with the control group at 10 weeks (p < 0.05). Collectively, dietary Gln supplementation (0.5% of concentration, as-fed basis) may not be influential enough to affect growth performance and gene expression related to muscle and adipose tissue development in steers. However, Gln supplementation increased the number of immune cells and decreased HSP90 in the hair follicle implying HS reduction in the corresponding group. |
| first_indexed | 2024-04-13T06:25:02Z |
| format | Article |
| id | doaj.art-cc7935620ddd4271bd58340a46362454 |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-04-13T06:25:02Z |
| publishDate | 2022-11-01 |
| publisher | Korean Society of Animal Sciences and Technology |
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| series | Journal of Animal Science and Technology |
| spelling | doaj.art-cc7935620ddd4271bd58340a463624542022-12-22T02:58:28ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912022-11-016461046106210.5187/jast.2022.e80Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditionsYves Kamali0Yong Ho Jo1Won-Seob Kim2Jalil Ghassemi Nejad3Jae-Sung Lee4Hong-Gu Lee5Department of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Animal Science, Michigan State University, East Lansing, MI 48824, USADepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaDepartment of Animal Science and Technology, Sanghuh College of Life Sciences, Konkuk University, Seoul 05029, KoreaThis study investigated the effects of L-glutamine (Gln) supplementation on growth performance, physiological traits, heat shock proteins (HSPs), and gene expression related to muscle and adipose tissue development in Hanwoo steers under heat stress (HS) conditions. Eight Hanwoo steers (initial body weight [BW] 570.7 ± 43.6 kg, months of age 22.3 ± 0.88) were randomly separated into two groups, control and treatment, and supplied with the concentration (1.5% of BW kg/day/head) and rice straw (1.5 kg/day/head). The treatment group were fed the Gln supplementation (0.5% of concentration, as-fed basis) once a day at 08:00 h. Blood samples for the assessment of haematological and biochemical parameters and the separation of peripheral blood mononuclear cells (PBMCs) were collected four times, at 0, 3, 6, and 10 weeks of the experiment. Feed intake was measured daily. BW to analyze growth performance and hair follicle collection to analyze the expression of HSPs were executed four times at 0, 3, 6, and 10 weeks. To analyze gene expression, longissimus dorsi muscle samples were collected by biopsy at the end of the study. As a result, growing performance, including final BW, average daily gain, and gain-to-feed ratio, were not different between the two groups. Leukocytes including lymphocytes and granulocytes, tended to increase in the Gln supplementation group (p = 0.058). There were also no differences in biochemical parameters shown between the two groups, except total protein and albumin, both of which were lower in the Gln supplementation group (p < 0.05). Gene expressions related to muscle and adipose tissue development were not different between the two groups. As temperature–humidity index (THI) increased, HSP70 and HSP90 expression in the hair follicle showed a high correlation. HSP90 in the hair follicle was decreased in the treatment group compared with the control group at 10 weeks (p < 0.05). Collectively, dietary Gln supplementation (0.5% of concentration, as-fed basis) may not be influential enough to affect growth performance and gene expression related to muscle and adipose tissue development in steers. However, Gln supplementation increased the number of immune cells and decreased HSP90 in the hair follicle implying HS reduction in the corresponding group. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e80L-GlutamineHanwoo steerHeat shock proteinHeat stressImmunity |
| spellingShingle | Yves Kamali Yong Ho Jo Won-Seob Kim Jalil Ghassemi Nejad Jae-Sung Lee Hong-Gu Lee Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions Journal of Animal Science and Technology L-Glutamine Hanwoo steer Heat shock protein Heat stress Immunity |
| title | Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions |
| title_full | Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions |
| title_fullStr | Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions |
| title_full_unstemmed | Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions |
| title_short | Dietary supplementation with L-glutamine enhances immunity and reduces heat stress in Hanwoo steers under heat stress conditions |
| title_sort | dietary supplementation with l glutamine enhances immunity and reduces heat stress in hanwoo steers under heat stress conditions |
| topic | L-Glutamine Hanwoo steer Heat shock protein Heat stress Immunity |
| url | http://www.ejast.org/archive/view_article?doi=10.5187/jast.2022.e80 |
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