Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress
Aminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxida...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-11-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/10/4/212 |
| _version_ | 1828117958327533568 |
|---|---|
| author | Eliana Pintus Martin Kadlec Marija Jovičić Markéta Sedmíková José Luis Ros-Santaella |
| author_facet | Eliana Pintus Martin Kadlec Marija Jovičić Markéta Sedmíková José Luis Ros-Santaella |
| author_sort | Eliana Pintus |
| collection | DOAJ |
| description | Aminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxidative stress are unknown. In this study, we tested the hypothesis that aminoguanidine may protect against the detrimental effects of oxidative stress in boar spermatozoa. For this purpose, sperm samples were incubated with a ROS generating system (Fe<sup>2+</sup>/ascorbate) with or without aminoguanidine supplementation (10, 1, and 0.1 mM). Our results show that aminoguanidine has powerful antioxidant capacity and protects boar spermatozoa against the deleterious effects of oxidative stress. After 2 h and 3.5 h of sperm incubation, the samples treated with aminoguanidine showed a significant increase in sperm velocity, plasma membrane and acrosome integrity together with a reduced lipid peroxidation in comparison with control samples (<i>p</i> < 0.001). Interestingly, except for the levels of malondialdehyde, the samples treated with 1 mM aminoguanidine did not differ or showed better performance than control samples without Fe<sup>2+</sup>/ascorbate. The results from this study provide new insights into the application of aminoguanidine as an in vitro therapeutic agent against the detrimental effects of oxidative stress in semen samples. |
| first_indexed | 2024-04-11T13:23:55Z |
| format | Article |
| id | doaj.art-cc7976411da04434908e83545f984208 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T13:23:55Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-cc7976411da04434908e83545f9842082022-12-22T04:22:07ZengMDPI AGPharmaceutics1999-49232018-11-0110421210.3390/pharmaceutics10040212pharmaceutics10040212Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative StressEliana Pintus0Martin Kadlec1Marija Jovičić2Markéta Sedmíková3José Luis Ros-Santaella4Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech RepublicAminoguanidine is a selective inhibitor of the inducible nitric oxide synthase (iNOS) and a scavenger of reactive oxygen species (ROS). Numerous studies have shown the antioxidant properties of aminoguanidine in several cell lines, but the in vitro effects of this compound on spermatozoa under oxidative stress are unknown. In this study, we tested the hypothesis that aminoguanidine may protect against the detrimental effects of oxidative stress in boar spermatozoa. For this purpose, sperm samples were incubated with a ROS generating system (Fe<sup>2+</sup>/ascorbate) with or without aminoguanidine supplementation (10, 1, and 0.1 mM). Our results show that aminoguanidine has powerful antioxidant capacity and protects boar spermatozoa against the deleterious effects of oxidative stress. After 2 h and 3.5 h of sperm incubation, the samples treated with aminoguanidine showed a significant increase in sperm velocity, plasma membrane and acrosome integrity together with a reduced lipid peroxidation in comparison with control samples (<i>p</i> < 0.001). Interestingly, except for the levels of malondialdehyde, the samples treated with 1 mM aminoguanidine did not differ or showed better performance than control samples without Fe<sup>2+</sup>/ascorbate. The results from this study provide new insights into the application of aminoguanidine as an in vitro therapeutic agent against the detrimental effects of oxidative stress in semen samples.https://www.mdpi.com/1999-4923/10/4/212antioxidant capacitylipid peroxidationnitric oxideoxidative stresssperm velocity |
| spellingShingle | Eliana Pintus Martin Kadlec Marija Jovičić Markéta Sedmíková José Luis Ros-Santaella Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress Pharmaceutics antioxidant capacity lipid peroxidation nitric oxide oxidative stress sperm velocity |
| title | Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress |
| title_full | Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress |
| title_fullStr | Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress |
| title_full_unstemmed | Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress |
| title_short | Aminoguanidine Protects Boar Spermatozoa against the Deleterious Effects of Oxidative Stress |
| title_sort | aminoguanidine protects boar spermatozoa against the deleterious effects of oxidative stress |
| topic | antioxidant capacity lipid peroxidation nitric oxide oxidative stress sperm velocity |
| url | https://www.mdpi.com/1999-4923/10/4/212 |
| work_keys_str_mv | AT elianapintus aminoguanidineprotectsboarspermatozoaagainstthedeleteriouseffectsofoxidativestress AT martinkadlec aminoguanidineprotectsboarspermatozoaagainstthedeleteriouseffectsofoxidativestress AT marijajovicic aminoguanidineprotectsboarspermatozoaagainstthedeleteriouseffectsofoxidativestress AT marketasedmikova aminoguanidineprotectsboarspermatozoaagainstthedeleteriouseffectsofoxidativestress AT joseluisrossantaella aminoguanidineprotectsboarspermatozoaagainstthedeleteriouseffectsofoxidativestress |