High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants

High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants

Abstract Polymerase chain reaction (PCR) variants requiring specific primer types are widely used in various PCR experiments, including generic PCR, inverse PCR, anchored PCR, and ARMS PCR. Few tools can be adapted for multiple PCR variants, and many tools select primers by filtration based on the g...

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Bibliographic Details
Main Authors: Huaping Zeng, Kexin Chen, Chouxian Ma, Biyin Zhu, Jun Chuan, Shuan Zhang, Lin Tang, Ting Yang, Zhaohui Sun, Xingkun Yang, Yu Wang
Format: Article
Language:English
Published: Nature Portfolio 2022-12-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-25561-z

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