In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i>
<i>Huperzia serrata</i> is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of <i>H. serrata</i> is considered a new way to relieve the resource pressure of <i...
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MDPI AG
2021-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/6/1112 |
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| author | Yan Yang Liangfang Dai Decai Wu Limin Dong Yisheng Tu Jiankun Xie Xiangdong Luo |
| author_facet | Yan Yang Liangfang Dai Decai Wu Limin Dong Yisheng Tu Jiankun Xie Xiangdong Luo |
| author_sort | Yan Yang |
| collection | DOAJ |
| description | <i>Huperzia serrata</i> is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of <i>H. serrata</i> is considered a new way to relieve the resource pressure of <i>H. serrata</i>. In this study, three different genotypic wild <i>H. serrata</i> were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated <i>H. serrata</i> were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for <i>H. serrata</i> was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L<sup>−1</sup> Naphthalene acetic acid (NAA) and 0.1 mg·L<sup>−1</sup> 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L<sup>−1</sup>), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g<sup>−1</sup>. The antioxidant experiment showed that the methanol extract of in vitro <i>H. serrata</i> had higher antioxidant activity than that of wild <i>H. serrata</i>. This study provides a reliable in vitro <i>H. serrata</i> culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA. |
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| series | Plants |
| spelling | doaj.art-cc7a3ef0432d47c3befff147aa99b5492023-11-21T22:14:31ZengMDPI AGPlants2223-77472021-05-01106111210.3390/plants10061112In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i>Yan Yang0Liangfang Dai1Decai Wu2Limin Dong3Yisheng Tu4Jiankun Xie5Xiangdong Luo6College of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, ChinaCollege of Life Science, Jiangxi Normal University, Nanchang 330022, China<i>Huperzia serrata</i> is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of <i>H. serrata</i> is considered a new way to relieve the resource pressure of <i>H. serrata</i>. In this study, three different genotypic wild <i>H. serrata</i> were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated <i>H. serrata</i> were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for <i>H. serrata</i> was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L<sup>−1</sup> Naphthalene acetic acid (NAA) and 0.1 mg·L<sup>−1</sup> 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L<sup>−1</sup>), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g<sup>−1</sup>. The antioxidant experiment showed that the methanol extract of in vitro <i>H. serrata</i> had higher antioxidant activity than that of wild <i>H. serrata</i>. This study provides a reliable in vitro <i>H. serrata</i> culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.https://www.mdpi.com/2223-7747/10/6/1112genotypes<i>Huperzia serrata</i>Huperzine Amicropropagationsterilizationantioxidant |
| spellingShingle | Yan Yang Liangfang Dai Decai Wu Limin Dong Yisheng Tu Jiankun Xie Xiangdong Luo In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> Plants genotypes <i>Huperzia serrata</i> Huperzine A micropropagation sterilization antioxidant |
| title | In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> |
| title_full | In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> |
| title_fullStr | In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> |
| title_full_unstemmed | In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> |
| title_short | In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic <i>Huperzia serrata</i> |
| title_sort | in vitro propagation huperzine a content and antioxidant activity of three genotypic i huperzia serrata i |
| topic | genotypes <i>Huperzia serrata</i> Huperzine A micropropagation sterilization antioxidant |
| url | https://www.mdpi.com/2223-7747/10/6/1112 |
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