3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress
Objective This work aims to screen drugs for preventing and treating vascular calcification. Method: We screened a series of 3-arylcoumarins for the detection of vascular calcification-associated factors using human aortic vascular smooth muscle cells.Results We found that compounds 14 and 32 signif...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/14756366.2022.2109024 |
| _version_ | 1811183691023515648 |
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| author | YuFei Li Yinbo Pan Liying Wang Xiaojing Wang Haiping Chu Yan Li Jie Sun Yanling Mu |
| author_facet | YuFei Li Yinbo Pan Liying Wang Xiaojing Wang Haiping Chu Yan Li Jie Sun Yanling Mu |
| author_sort | YuFei Li |
| collection | DOAJ |
| description | Objective This work aims to screen drugs for preventing and treating vascular calcification. Method: We screened a series of 3-arylcoumarins for the detection of vascular calcification-associated factors using human aortic vascular smooth muscle cells.Results We found that compounds 14 and 32 significantly inhibited alkaline phosphatase (ALP) activity similar to aminoguanidine hydrochloride (AGH) in a cellular model of AGEs-induced calcification. We also found that compounds 14 and 32 could significantly decrease the levels of factors such as AGEs, intracellular calcium ions, and total ROS in the calcified cell model. Further study indicates that compound 14 could significantly inhibit the expression of P-ERK1/2, PKC, NF-κB, RAGE and OPN proteins and increased the expression of SM22-α and PPAR-γ proteins in the calcified cells.Conclusion We speculate that compound 14 inhibits vascular calcification by inhibiting oxidative stress and inhibiting AGEs production, suggesting that 3-arylcoumarin derivatives are potential candidates for the treatment of vascular calcification. |
| first_indexed | 2024-04-11T09:51:06Z |
| format | Article |
| id | doaj.art-cc7ab4ce3dad4ede90b3c817e0577b0f |
| institution | Directory Open Access Journal |
| issn | 1475-6366 1475-6374 |
| language | English |
| last_indexed | 2024-04-11T09:51:06Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj.art-cc7ab4ce3dad4ede90b3c817e0577b0f2022-12-22T04:30:48ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742022-12-013712147215710.1080/14756366.2022.21090243-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stressYuFei Li0Yinbo Pan1Liying Wang2Xiaojing Wang3Haiping Chu4Yan Li5Jie Sun6Yanling Mu7Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaShandong Electric Power Central Hospital, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, ChinaObjective This work aims to screen drugs for preventing and treating vascular calcification. Method: We screened a series of 3-arylcoumarins for the detection of vascular calcification-associated factors using human aortic vascular smooth muscle cells.Results We found that compounds 14 and 32 significantly inhibited alkaline phosphatase (ALP) activity similar to aminoguanidine hydrochloride (AGH) in a cellular model of AGEs-induced calcification. We also found that compounds 14 and 32 could significantly decrease the levels of factors such as AGEs, intracellular calcium ions, and total ROS in the calcified cell model. Further study indicates that compound 14 could significantly inhibit the expression of P-ERK1/2, PKC, NF-κB, RAGE and OPN proteins and increased the expression of SM22-α and PPAR-γ proteins in the calcified cells.Conclusion We speculate that compound 14 inhibits vascular calcification by inhibiting oxidative stress and inhibiting AGEs production, suggesting that 3-arylcoumarin derivatives are potential candidates for the treatment of vascular calcification.https://www.tandfonline.com/doi/10.1080/14756366.2022.21090243-arylcoumarin derivativesvascular calcificationAGEsoxidative stress |
| spellingShingle | YuFei Li Yinbo Pan Liying Wang Xiaojing Wang Haiping Chu Yan Li Jie Sun Yanling Mu 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress Journal of Enzyme Inhibition and Medicinal Chemistry 3-arylcoumarin derivatives vascular calcification AGEs oxidative stress |
| title | 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress |
| title_full | 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress |
| title_fullStr | 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress |
| title_full_unstemmed | 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress |
| title_short | 3-Arylcoumarin inhibits vascular calcification by inhibiting the generation of AGEs and anti-oxidative stress |
| title_sort | 3 arylcoumarin inhibits vascular calcification by inhibiting the generation of ages and anti oxidative stress |
| topic | 3-arylcoumarin derivatives vascular calcification AGEs oxidative stress |
| url | https://www.tandfonline.com/doi/10.1080/14756366.2022.2109024 |
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