A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
Access to glycoenzymes for basic and applied research is limited by difficulties with their recombinant expression. Here, the authors describe a universal strategy for converting membrane-bound glycosyltransferases into water-soluble biocatalysts, which are expressed at high levels with retention of...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34029-7 |
| _version_ | 1811197860031496192 |
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| author | Thapakorn Jaroentomeechai Yong Hyun Kwon Yiwen Liu Olivia Young Ruchika Bhawal Joshua D. Wilson Mingji Li Digantkumar G. Chapla Kelley W. Moremen Michael C. Jewett Dario Mizrachi Matthew P. DeLisa |
| author_facet | Thapakorn Jaroentomeechai Yong Hyun Kwon Yiwen Liu Olivia Young Ruchika Bhawal Joshua D. Wilson Mingji Li Digantkumar G. Chapla Kelley W. Moremen Michael C. Jewett Dario Mizrachi Matthew P. DeLisa |
| author_sort | Thapakorn Jaroentomeechai |
| collection | DOAJ |
| description | Access to glycoenzymes for basic and applied research is limited by difficulties with their recombinant expression. Here, the authors describe a universal strategy for converting membrane-bound glycosyltransferases into water-soluble biocatalysts, which are expressed at high levels with retention of activity. |
| first_indexed | 2024-04-12T01:20:13Z |
| format | Article |
| id | doaj.art-cc7e68d73dbc4e25965f65c3d9808c86 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-12T01:20:13Z |
| publishDate | 2022-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-cc7e68d73dbc4e25965f65c3d9808c862022-12-22T03:53:48ZengNature PortfolioNature Communications2041-17232022-10-0113111710.1038/s41467-022-34029-7A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycansThapakorn Jaroentomeechai0Yong Hyun Kwon1Yiwen Liu2Olivia Young3Ruchika Bhawal4Joshua D. Wilson5Mingji Li6Digantkumar G. Chapla7Kelley W. Moremen8Michael C. Jewett9Dario Mizrachi10Matthew P. DeLisa11Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityRobert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityRobert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityRobert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityCornell Institute of Biotechnology, Cornell UniversityGlycobia, Inc.Robert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityComplex Carbohydrate Research Center, University of GeorgiaComplex Carbohydrate Research Center, University of GeorgiaDepartment of Chemical and Biological Engineering, Northwestern UniversityDepartment of Physiology & Developmental Biology, Brigham Young UniversityRobert F. Smith School of Chemical and Biomolecular Engineering, Cornell UniversityAccess to glycoenzymes for basic and applied research is limited by difficulties with their recombinant expression. Here, the authors describe a universal strategy for converting membrane-bound glycosyltransferases into water-soluble biocatalysts, which are expressed at high levels with retention of activity.https://doi.org/10.1038/s41467-022-34029-7 |
| spellingShingle | Thapakorn Jaroentomeechai Yong Hyun Kwon Yiwen Liu Olivia Young Ruchika Bhawal Joshua D. Wilson Mingji Li Digantkumar G. Chapla Kelley W. Moremen Michael C. Jewett Dario Mizrachi Matthew P. DeLisa A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans Nature Communications |
| title | A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans |
| title_full | A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans |
| title_fullStr | A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans |
| title_full_unstemmed | A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans |
| title_short | A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans |
| title_sort | universal glycoenzyme biosynthesis pipeline that enables efficient cell free remodeling of glycans |
| url | https://doi.org/10.1038/s41467-022-34029-7 |
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