A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis
Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous system has been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00384/full |
| _version_ | 1818992020888223744 |
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| author | Nuno Faria-Blanc Jenny C. Mortimer Jenny C. Mortimer Jenny C. Mortimer Paul Dupree |
| author_facet | Nuno Faria-Blanc Jenny C. Mortimer Jenny C. Mortimer Jenny C. Mortimer Paul Dupree |
| author_sort | Nuno Faria-Blanc |
| collection | DOAJ |
| description | Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous system has been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants. |
| first_indexed | 2024-12-20T20:19:32Z |
| format | Article |
| id | doaj.art-cc804bb99d494c06a4af3a21bea0b8c2 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-20T20:19:32Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-cc804bb99d494c06a4af3a21bea0b8c22022-12-21T19:27:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00384348033A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in ArabidopsisNuno Faria-Blanc0Jenny C. Mortimer1Jenny C. Mortimer2Jenny C. Mortimer3Paul Dupree4Department of Biochemistry, University of Cambridge, Cambridge, United KingdomDepartment of Biochemistry, University of Cambridge, Cambridge, United KingdomBiosciences Area, Lawrence Berkeley National Laboratory, Berkeley, CA, United StatesJoint BioEnergy Institute, Emeryville, CA, United StatesDepartment of Biochemistry, University of Cambridge, Cambridge, United KingdomYeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous system has been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants.http://journal.frontiersin.org/article/10.3389/fpls.2018.00384/fullxylancell wall engineeringcell wall integrityplantsecondary cell wallbiofuels |
| spellingShingle | Nuno Faria-Blanc Jenny C. Mortimer Jenny C. Mortimer Jenny C. Mortimer Paul Dupree A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis Frontiers in Plant Science xylan cell wall engineering cell wall integrity plant secondary cell wall biofuels |
| title | A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis |
| title_full | A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis |
| title_fullStr | A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis |
| title_full_unstemmed | A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis |
| title_short | A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of a Secondary Cell Wall Integrity System in Arabidopsis |
| title_sort | transcriptomic analysis of xylan mutants does not support the existence of a secondary cell wall integrity system in arabidopsis |
| topic | xylan cell wall engineering cell wall integrity plant secondary cell wall biofuels |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00384/full |
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