CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants
Photosystem II repair in chloroplasts is a critical process involved in maintaining a plant’s photosynthetic activity under cold stress. FtsH (filamentation temperature-sensitive H) is an essential metalloprotease that is required for chloroplast photosystem II repair. However, the role of FtsH in t...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6288 |
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| author | Taimei Di Yedie Wu Jing Peng Jie Wang Haoqian Wang Mingming He Nana Li Xinyuan Hao Yajun Yang Dejiang Ni Lu Wang Xinchao Wang |
| author_facet | Taimei Di Yedie Wu Jing Peng Jie Wang Haoqian Wang Mingming He Nana Li Xinyuan Hao Yajun Yang Dejiang Ni Lu Wang Xinchao Wang |
| author_sort | Taimei Di |
| collection | DOAJ |
| description | Photosystem II repair in chloroplasts is a critical process involved in maintaining a plant’s photosynthetic activity under cold stress. FtsH (filamentation temperature-sensitive H) is an essential metalloprotease that is required for chloroplast photosystem II repair. However, the role of FtsH in tea plants and its regulatory mechanism under cold stress remains elusive. In this study, we cloned a <i>FtsH</i> homolog gene in tea plants, named <i>CsFtsH5</i>, and found that CsFtsH5 was located in the chloroplast and cytomembrane. RT-qPCR showed that the expression of <i>CsFtsH5</i> was increased with leaf maturity and was significantly induced by light and cold stress. Transient knockdown <i>CsFtsH5</i> expression in tea leaves using antisense oligonucleotides resulted in hypersensitivity to cold stress, along with higher relative electrolyte leakage and lower <i>Fv/Fm</i> values. To investigate the molecular mechanism underlying <i>CsFtsH5</i> involvement in the cold stress, we focused on the calcineurin B-like-interacting protein kinase 11 (<i>CsCIPK11</i>), which had a tissue expression pattern similar to that of <i>CsFtsH5</i> and was also upregulated by light and cold stress. Yeast two-hybrid and dual luciferase (Luc) complementation assays revealed that CsFtsH5 interacted with CsCIPK11. Furthermore, the Dual-Luc assay showed that CsCIPK11-CsFtsH5 interaction might enhance CsFtsH5 stability. Altogether, our study demonstrates that <i>CsFtsH5</i> is associated with <i>CsCIPK11</i> and plays a positive role in maintaining the photosynthetic activity of tea plants in response to low temperatures. |
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| last_indexed | 2024-03-11T05:36:13Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-cc8076692e7d4c78adcea718d1b619ff2023-11-17T16:49:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247628810.3390/ijms24076288CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea PlantsTaimei Di0Yedie Wu1Jing Peng2Jie Wang3Haoqian Wang4Mingming He5Nana Li6Xinyuan Hao7Yajun Yang8Dejiang Ni9Lu Wang10Xinchao Wang11Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaCollege of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaKey Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, 9th South of Meiling Road, Hangzhou 310008, ChinaPhotosystem II repair in chloroplasts is a critical process involved in maintaining a plant’s photosynthetic activity under cold stress. FtsH (filamentation temperature-sensitive H) is an essential metalloprotease that is required for chloroplast photosystem II repair. However, the role of FtsH in tea plants and its regulatory mechanism under cold stress remains elusive. In this study, we cloned a <i>FtsH</i> homolog gene in tea plants, named <i>CsFtsH5</i>, and found that CsFtsH5 was located in the chloroplast and cytomembrane. RT-qPCR showed that the expression of <i>CsFtsH5</i> was increased with leaf maturity and was significantly induced by light and cold stress. Transient knockdown <i>CsFtsH5</i> expression in tea leaves using antisense oligonucleotides resulted in hypersensitivity to cold stress, along with higher relative electrolyte leakage and lower <i>Fv/Fm</i> values. To investigate the molecular mechanism underlying <i>CsFtsH5</i> involvement in the cold stress, we focused on the calcineurin B-like-interacting protein kinase 11 (<i>CsCIPK11</i>), which had a tissue expression pattern similar to that of <i>CsFtsH5</i> and was also upregulated by light and cold stress. Yeast two-hybrid and dual luciferase (Luc) complementation assays revealed that CsFtsH5 interacted with CsCIPK11. Furthermore, the Dual-Luc assay showed that CsCIPK11-CsFtsH5 interaction might enhance CsFtsH5 stability. Altogether, our study demonstrates that <i>CsFtsH5</i> is associated with <i>CsCIPK11</i> and plays a positive role in maintaining the photosynthetic activity of tea plants in response to low temperatures.https://www.mdpi.com/1422-0067/24/7/6288coldCsCIPK11CsFtsH5photosynthetic activitytea plant |
| spellingShingle | Taimei Di Yedie Wu Jing Peng Jie Wang Haoqian Wang Mingming He Nana Li Xinyuan Hao Yajun Yang Dejiang Ni Lu Wang Xinchao Wang CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants International Journal of Molecular Sciences cold CsCIPK11 CsFtsH5 photosynthetic activity tea plant |
| title | CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants |
| title_full | CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants |
| title_fullStr | CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants |
| title_full_unstemmed | CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants |
| title_short | CsCIPK11-Regulated Metalloprotease CsFtsH5 Mediates the Cold Response of Tea Plants |
| title_sort | cscipk11 regulated metalloprotease csftsh5 mediates the cold response of tea plants |
| topic | cold CsCIPK11 CsFtsH5 photosynthetic activity tea plant |
| url | https://www.mdpi.com/1422-0067/24/7/6288 |
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