Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels
Abstract Introduction Peste des petits ruminants virus (PPRV) causes a highly devastating disease of sheep and goats, peste des petits ruminants (PPR), which is targeted for global control and eradication by 2030. The serological diagnostic tool kits for accurate diagnosis of PPR have inherent stren...
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02669-w |
| _version_ | 1797986046643798016 |
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| author | Kumela Lelisa Tesfaye Rufael Chibssa Fanta Desissa Kemal Emiyu Ayelech Muluneh Demeke Sibhatu Lobago Dereje Shegu Gebreweld Kebede Debebe Abde Aliy Mohammed |
| author_facet | Kumela Lelisa Tesfaye Rufael Chibssa Fanta Desissa Kemal Emiyu Ayelech Muluneh Demeke Sibhatu Lobago Dereje Shegu Gebreweld Kebede Debebe Abde Aliy Mohammed |
| author_sort | Kumela Lelisa |
| collection | DOAJ |
| description | Abstract Introduction Peste des petits ruminants virus (PPRV) causes a highly devastating disease of sheep and goats, peste des petits ruminants (PPR), which is targeted for global control and eradication by 2030. The serological diagnostic tool kits for accurate diagnosis of PPR have inherent strengths and weaknesses that require parallel validation and optimization across animal species. Thus, the objective of this study was to evaluate diagnostic performance of haemagglutinin based PPR blocking ELISA (HPPR- b-ELISA), that was developed by Africa Union Pan African Veterinary Vaccine Center for specific detection of anti- PPRV antibodies. Methods In preliminarily investigation, diagnostic performance of the HPPR-b-ELISA®, commercial PPR competition ELISA (c-ELISA) and virus neutralization test (VNT) were compared for the detection of anti-PPRV antibodies in goats, sheep, cattle and camels. Results The sensitivity and specificity of HPPR- b-ELISA® were 79.55 and 99.74%, respectively, compared to c-ELISA. The HPPR- b-ELISA® was in perfect agreement (κ = 0.86) with the c-ELISA in all sera collected from goats, sheep and cattle. There was almost perfect agreement between the species of goats (κ = 0.82) and sheep (κ = 0.98), while the agreement was substantial in cattle (κ = 0.78) and no agreement was observed in camels (κ = 0.00). Similarly, the sensitivity and specificity of the HPPR b-ELISA were 80 and 96.36%, respectively compared to VNT with almost perfect agreement in goats (κ = 0.83) and sheep (κ = 0.89), moderate in cattle (κ = 0.50) and none in camels (κ = 0.00). Conclusion Our study revealed that HPPR- b-ELISA is a suitable and valid method that can alternatively be used for screening and monitoring of PPR in sheep, goats and cattle except for camels. |
| first_indexed | 2024-04-11T07:26:54Z |
| format | Article |
| id | doaj.art-cc858aa6fd5a402c928528bf209fa37a |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T07:26:54Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-cc858aa6fd5a402c928528bf209fa37a2022-12-22T04:37:02ZengBMCBMC Microbiology1471-21802022-10-012211610.1186/s12866-022-02669-wEvaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camelsKumela Lelisa0Tesfaye Rufael Chibssa1Fanta Desissa2Kemal Emiyu3Ayelech Muluneh4Demeke Sibhatu Lobago5Dereje Shegu Gebreweld6Kebede Debebe7Abde Aliy Mohammed8Animal Health InstituteAnimal Health InstituteCollege of Veterinary Medicine and Agriculture, Department of Microbiology, Immunology and Veterinary Public health, Addis Ababa UniversityAnimal Health InstituteAnimal Health InstituteAnimal Health InstituteAnimal Health InstituteAnimal Health InstituteAnimal Health InstituteAbstract Introduction Peste des petits ruminants virus (PPRV) causes a highly devastating disease of sheep and goats, peste des petits ruminants (PPR), which is targeted for global control and eradication by 2030. The serological diagnostic tool kits for accurate diagnosis of PPR have inherent strengths and weaknesses that require parallel validation and optimization across animal species. Thus, the objective of this study was to evaluate diagnostic performance of haemagglutinin based PPR blocking ELISA (HPPR- b-ELISA), that was developed by Africa Union Pan African Veterinary Vaccine Center for specific detection of anti- PPRV antibodies. Methods In preliminarily investigation, diagnostic performance of the HPPR-b-ELISA®, commercial PPR competition ELISA (c-ELISA) and virus neutralization test (VNT) were compared for the detection of anti-PPRV antibodies in goats, sheep, cattle and camels. Results The sensitivity and specificity of HPPR- b-ELISA® were 79.55 and 99.74%, respectively, compared to c-ELISA. The HPPR- b-ELISA® was in perfect agreement (κ = 0.86) with the c-ELISA in all sera collected from goats, sheep and cattle. There was almost perfect agreement between the species of goats (κ = 0.82) and sheep (κ = 0.98), while the agreement was substantial in cattle (κ = 0.78) and no agreement was observed in camels (κ = 0.00). Similarly, the sensitivity and specificity of the HPPR b-ELISA were 80 and 96.36%, respectively compared to VNT with almost perfect agreement in goats (κ = 0.83) and sheep (κ = 0.89), moderate in cattle (κ = 0.50) and none in camels (κ = 0.00). Conclusion Our study revealed that HPPR- b-ELISA is a suitable and valid method that can alternatively be used for screening and monitoring of PPR in sheep, goats and cattle except for camels.https://doi.org/10.1186/s12866-022-02669-wPeste des petits ruminantsRuminantsHPPR- b-ELISAc-ELISAVNTAntibodies |
| spellingShingle | Kumela Lelisa Tesfaye Rufael Chibssa Fanta Desissa Kemal Emiyu Ayelech Muluneh Demeke Sibhatu Lobago Dereje Shegu Gebreweld Kebede Debebe Abde Aliy Mohammed Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels BMC Microbiology Peste des petits ruminants Ruminants HPPR- b-ELISA c-ELISA VNT Antibodies |
| title | Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels |
| title_full | Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels |
| title_fullStr | Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels |
| title_full_unstemmed | Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels |
| title_short | Evaluation of diagnostic performance of H-based blocking ELISA for specific detection of peste des petits ruminants in domestic sheep, goats, cattle and camels |
| title_sort | evaluation of diagnostic performance of h based blocking elisa for specific detection of peste des petits ruminants in domestic sheep goats cattle and camels |
| topic | Peste des petits ruminants Ruminants HPPR- b-ELISA c-ELISA VNT Antibodies |
| url | https://doi.org/10.1186/s12866-022-02669-w |
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