Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances
The greatest challenge of the current generation and generations to come is antimicrobial resistance, as different pathogenic bacteria have continuously evolved to become resistant to even the most recently synthesized antibiotics such as carbapenems. Resistance to carbapenems limits the therapeutic...
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| Format: | Article |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.820218/full |
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| author | Kenneth Ssekatawa Denis K. Byarugaba Martin Kamilo Angwe Eddie M. Wampande Francis Ejobi Edward Nxumalo Malik Maaza Malik Maaza Juliet Sackey Juliet Sackey John Baptist Kirabira |
| author_facet | Kenneth Ssekatawa Denis K. Byarugaba Martin Kamilo Angwe Eddie M. Wampande Francis Ejobi Edward Nxumalo Malik Maaza Malik Maaza Juliet Sackey Juliet Sackey John Baptist Kirabira |
| author_sort | Kenneth Ssekatawa |
| collection | DOAJ |
| description | The greatest challenge of the current generation and generations to come is antimicrobial resistance, as different pathogenic bacteria have continuously evolved to become resistant to even the most recently synthesized antibiotics such as carbapenems. Resistance to carbapenems limits the therapeutic options of MDR infections as they are the only safe and effective drugs recommended to treat such infections. This scenario has complicated treatment outcomes, even to the commonest bacterial infections. Repeated attempts to develop other approaches have been made. The most promising novel therapeutic option is the use of nanomaterials as antimicrobial agents. Thus, this study examined the efficacy of Camellia sinensis extract (CSE) and Prunus africana bark extract (PAE) green synthesized Copper oxide nanoparticles (CuONPs) against carbapenem-resistant bacteria. Furthermore, the photocatalytic and antioxidant activities of CuONPs were evaluated to determine the potential of using them in a wide range of applications. CuONPs were biosynthesized by CSE and PAE. UV vis spectroscopy, X-ray Diffraction (XRD), Dynamic light scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were used to characterize the nanoparticles. CuONPs susceptibility tests were carried out by the agar well diffusion method. The photocatalytic and antioxidant activities of the CuONPs were determined by the methylene blue and DPPH free radical scavenging assays, respectively. UV vis absorbance spectra registered surface plasmon resonance peaks between 272 and 286 nm, confirming the presence of CuONPs. The XRD array had nine strong peaks at 2θ values typical of CuONPs. FTIR spectra exhibited bands associated with organic functional groups confirming capping and functionalization of the CuONPs by the phytochemicals. DLS analysis registered a net zeta potential of +12.5 mV. SEM analysis revealed that the nanoparticles were spherical and clustered with a mean diameter of 6 nm. Phytosynthesized CuONPs exhibited the highest growth suppression zones of 30 mm with MIC ranging from 30 to 125 μg/ml against MDR bacteria. Furthermore, the CuONPs achieved a methylene blue dye photocatalysis degradation efficiency of 85.5% and a free radical scavenging activity of 28.8%. PAE and CSE successfully bio-reduced copper ions to the nanoscale level with potent antimicrobial, photocatalysis, and antioxidant activities. |
| first_indexed | 2024-12-17T10:33:57Z |
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| id | doaj.art-cc88990f92c54e239832198b5633b5da |
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| language | English |
| last_indexed | 2024-12-17T10:33:57Z |
| publishDate | 2022-02-01 |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-cc88990f92c54e239832198b5633b5da2022-12-21T21:52:27ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-02-011010.3389/fbioe.2022.820218820218Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic PerformancesKenneth Ssekatawa0Denis K. Byarugaba1Martin Kamilo Angwe2Eddie M. Wampande3Francis Ejobi4Edward Nxumalo5Malik Maaza6Malik Maaza7Juliet Sackey8Juliet Sackey9John Baptist Kirabira10College of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, Kampala, UgandaCollege of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, Kampala, UgandaCollege of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, Kampala, UgandaCollege of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, Kampala, UgandaCollege of Veterinary Medicine Animal Resources and Biosecurity, Makerere University, Kampala, UgandaInstitute for Nanotechnology and Water Sustainability, College of Science, Engineering and Technology, Florida Science Campus, University of South Africa, Pretoria, South AfricaNanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, Somerset West, South AfricaUNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology, College of Graduate Studies, University of South Africa (UNISA), Pretoria, South AfricaNanosciences African Network (NANOAFNET), iThemba LABS-National Research Foundation, Somerset West, South AfricaUNESCO-UNISA Africa Chair in Nanosciences/Nanotechnology, College of Graduate Studies, University of South Africa (UNISA), Pretoria, South AfricaAfrica Center of Excellence in Materials, Product Development and Nanotechnology, College of Engineering, Design, Art and Technology, Makerere University, Kampala, UgandaThe greatest challenge of the current generation and generations to come is antimicrobial resistance, as different pathogenic bacteria have continuously evolved to become resistant to even the most recently synthesized antibiotics such as carbapenems. Resistance to carbapenems limits the therapeutic options of MDR infections as they are the only safe and effective drugs recommended to treat such infections. This scenario has complicated treatment outcomes, even to the commonest bacterial infections. Repeated attempts to develop other approaches have been made. The most promising novel therapeutic option is the use of nanomaterials as antimicrobial agents. Thus, this study examined the efficacy of Camellia sinensis extract (CSE) and Prunus africana bark extract (PAE) green synthesized Copper oxide nanoparticles (CuONPs) against carbapenem-resistant bacteria. Furthermore, the photocatalytic and antioxidant activities of CuONPs were evaluated to determine the potential of using them in a wide range of applications. CuONPs were biosynthesized by CSE and PAE. UV vis spectroscopy, X-ray Diffraction (XRD), Dynamic light scattering (DLS), Fourier Transform Infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were used to characterize the nanoparticles. CuONPs susceptibility tests were carried out by the agar well diffusion method. The photocatalytic and antioxidant activities of the CuONPs were determined by the methylene blue and DPPH free radical scavenging assays, respectively. UV vis absorbance spectra registered surface plasmon resonance peaks between 272 and 286 nm, confirming the presence of CuONPs. The XRD array had nine strong peaks at 2θ values typical of CuONPs. FTIR spectra exhibited bands associated with organic functional groups confirming capping and functionalization of the CuONPs by the phytochemicals. DLS analysis registered a net zeta potential of +12.5 mV. SEM analysis revealed that the nanoparticles were spherical and clustered with a mean diameter of 6 nm. Phytosynthesized CuONPs exhibited the highest growth suppression zones of 30 mm with MIC ranging from 30 to 125 μg/ml against MDR bacteria. Furthermore, the CuONPs achieved a methylene blue dye photocatalysis degradation efficiency of 85.5% and a free radical scavenging activity of 28.8%. PAE and CSE successfully bio-reduced copper ions to the nanoscale level with potent antimicrobial, photocatalysis, and antioxidant activities.https://www.frontiersin.org/articles/10.3389/fbioe.2022.820218/fullantibiotic resistancephotocatalysisantioxidant activitygreen synthesis of nanoparticlescopper oxide nanoparticlesPrunus africana |
| spellingShingle | Kenneth Ssekatawa Denis K. Byarugaba Martin Kamilo Angwe Eddie M. Wampande Francis Ejobi Edward Nxumalo Malik Maaza Malik Maaza Juliet Sackey Juliet Sackey John Baptist Kirabira Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances Frontiers in Bioengineering and Biotechnology antibiotic resistance photocatalysis antioxidant activity green synthesis of nanoparticles copper oxide nanoparticles Prunus africana |
| title | Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances |
| title_full | Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances |
| title_fullStr | Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances |
| title_full_unstemmed | Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances |
| title_short | Phyto-Mediated Copper Oxide Nanoparticles for Antibacterial, Antioxidant and Photocatalytic Performances |
| title_sort | phyto mediated copper oxide nanoparticles for antibacterial antioxidant and photocatalytic performances |
| topic | antibiotic resistance photocatalysis antioxidant activity green synthesis of nanoparticles copper oxide nanoparticles Prunus africana |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.820218/full |
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