Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential
A total of 296 isolates of <i>Saccharomyces cerevisiae</i> sampled from naturally fermenting grape musts from various locations in Lebanon were typed by interdelta fingerprinting. Of these, 88 isolates were compared with oenological strains originating from various countries, using micro...
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MDPI AG
2021-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/8/678 |
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| author | Marie-José Ayoub Jean-Luc Legras Pierre Abi-Nakhoul Huu-Vang Nguyen Rachad Saliba Claude Gaillardin |
| author_facet | Marie-José Ayoub Jean-Luc Legras Pierre Abi-Nakhoul Huu-Vang Nguyen Rachad Saliba Claude Gaillardin |
| author_sort | Marie-José Ayoub |
| collection | DOAJ |
| description | A total of 296 isolates of <i>Saccharomyces cerevisiae</i> sampled from naturally fermenting grape musts from various locations in Lebanon were typed by interdelta fingerprinting. Of these, 88 isolates were compared with oenological strains originating from various countries, using microsatellite characterization at six polymorphic loci. These approaches evidenced a large diversity of the natural oenological Lebanese flora over the territory as well as in individual spontaneous fermentations. Several cases of dominance and perenniality of isolates were observed in the same wineries, where fermentations appeared to involve lineages of sibling isolates. Our work thus evidenced a “winery effect” on strains’ relatedness. Similarly, related or identical strains were also detected in vicinal wineries, suggesting strain circulation within small geographical areas and a further “vicinity effect”. Moreover, and despite its diversity, the Lebanese flora seemed interrelated, on the basis of microsatellite loci analysis, in comparison to worldwide communities. We finally tested the ability of 21 indigenous strains to act as potential starters for winemaking. Seven of them passed our pre-selection scheme and two of them at least may be good candidates for use provided pilot-scale assays confirm their suitability. |
| first_indexed | 2024-03-10T08:41:21Z |
| format | Article |
| id | doaj.art-cc8a1a6e65244333ae32ffbe4a52c367 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T08:41:21Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-cc8a1a6e65244333ae32ffbe4a52c3672023-11-22T08:17:45ZengMDPI AGJournal of Fungi2309-608X2021-08-017867810.3390/jof7080678Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking PotentialMarie-José Ayoub0Jean-Luc Legras1Pierre Abi-Nakhoul2Huu-Vang Nguyen3Rachad Saliba4Claude Gaillardin5Department of Food Sciences and Technologies, Faculty of Agricultural and Veterinary Sciences, Lebanese University, Beirut 14-6573, LebanonSPO, University of Montpellier, INRAE, Institut Agro, F-34060 Montpellier, FranceDepartment of Food Sciences and Technologies, Faculty of Agricultural and Veterinary Sciences, Lebanese University, Beirut 14-6573, LebanonCIRM-Levures, SPO, University of Montpellier, INRAE, Institut Agro, F-34060 Montpellier, FranceDepartment of Food Sciences and Technologies, Faculty of Agricultural and Veterinary Sciences, Lebanese University, Beirut 14-6573, LebanonAgroParisTech, Micalis UMR 1319, CBAI, F-78850 Thiverval-Grignon, FranceA total of 296 isolates of <i>Saccharomyces cerevisiae</i> sampled from naturally fermenting grape musts from various locations in Lebanon were typed by interdelta fingerprinting. Of these, 88 isolates were compared with oenological strains originating from various countries, using microsatellite characterization at six polymorphic loci. These approaches evidenced a large diversity of the natural oenological Lebanese flora over the territory as well as in individual spontaneous fermentations. Several cases of dominance and perenniality of isolates were observed in the same wineries, where fermentations appeared to involve lineages of sibling isolates. Our work thus evidenced a “winery effect” on strains’ relatedness. Similarly, related or identical strains were also detected in vicinal wineries, suggesting strain circulation within small geographical areas and a further “vicinity effect”. Moreover, and despite its diversity, the Lebanese flora seemed interrelated, on the basis of microsatellite loci analysis, in comparison to worldwide communities. We finally tested the ability of 21 indigenous strains to act as potential starters for winemaking. Seven of them passed our pre-selection scheme and two of them at least may be good candidates for use provided pilot-scale assays confirm their suitability.https://www.mdpi.com/2309-608X/7/8/678<i>Saccharomyces cerevisiae</i>biodiversitynatural fermentationindigenous yeastwine aroma |
| spellingShingle | Marie-José Ayoub Jean-Luc Legras Pierre Abi-Nakhoul Huu-Vang Nguyen Rachad Saliba Claude Gaillardin Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential Journal of Fungi <i>Saccharomyces cerevisiae</i> biodiversity natural fermentation indigenous yeast wine aroma |
| title | Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential |
| title_full | Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential |
| title_fullStr | Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential |
| title_full_unstemmed | Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential |
| title_short | Lebanon’s Native Oenological <i>Saccharomyces cerevisiae</i> Flora: Assessment of Different Aspects of Genetic Diversity and Evaluation of Winemaking Potential |
| title_sort | lebanon s native oenological i saccharomyces cerevisiae i flora assessment of different aspects of genetic diversity and evaluation of winemaking potential |
| topic | <i>Saccharomyces cerevisiae</i> biodiversity natural fermentation indigenous yeast wine aroma |
| url | https://www.mdpi.com/2309-608X/7/8/678 |
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