Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation
Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively...
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| Format: | Article |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1138762/full |
| _version_ | 1797868243092766720 |
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| author | Youzhen Wei Youzhen Wei Youzhen Wei Kun Wang Yafang Zhang Yi Duan Yan Tian Hongling Yin Xuelian Fu Zuan Ma Jianjun Zhou Min Yu Qingbin Ni Wenjie Tang Wenjie Tang |
| author_facet | Youzhen Wei Youzhen Wei Youzhen Wei Kun Wang Yafang Zhang Yi Duan Yan Tian Hongling Yin Xuelian Fu Zuan Ma Jianjun Zhou Min Yu Qingbin Ni Wenjie Tang Wenjie Tang |
| author_sort | Youzhen Wei |
| collection | DOAJ |
| description | Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol. |
| first_indexed | 2024-04-09T23:52:55Z |
| format | Article |
| id | doaj.art-cc8c33051a7e4ea7a5b1ef9b1c8389d0 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-09T23:52:55Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-cc8c33051a7e4ea7a5b1ef9b1c8389d02023-03-17T05:00:41ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-03-011410.3389/fphar.2023.11387621138762Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammationYouzhen Wei0Youzhen Wei1Youzhen Wei2Kun Wang3Yafang Zhang4Yi Duan5Yan Tian6Hongling Yin7Xuelian Fu8Zuan Ma9Jianjun Zhou10Min Yu11Qingbin Ni12Wenjie Tang13Wenjie Tang14Research Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaHydrogen Medicine Center, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong, ChinaResearch Center for Translational Medicine, Jinan People’s Hospital, Shandong First Medical University, Jinan, Shandong, ChinaOffice of Academic Research, Taishan Vocational College of Nursing, Taian, Shandong, ChinaDepartment of Neonatology and NICU, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaThe Key Laboratory of Metabolism and Molecular Medicine, The Ministry of Education, Department of Biochemistry and Molecular Biology, School of Basic Medicine, Fudan University, Shanghai, ChinaHydrogen Medicine Center, The Affiliated Taian City Central Hospital of Qingdao University, Taian, Shandong, ChinaResearch Institute of Heart Failure, Research Center for Translational Medicine & Key Laboratory of Arrhythmias of the Ministry of Education of China, East Hospital, Tongji University School of Medicine, Shanghai, ChinaResearch Institute of Regenerative Medicine, East Hospital, Tongji University, Shanghai, ChinaIntroduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice. However, the early dynamic process of the inflammatory response caused by lipopolysaccharide (LPS) and the anti-inflammatory effect of hydrogen has not been definitively reported. Methods: Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken. Pathological changes in lung tissue were assessed using hematoxylin and eosin (HE) staining. Levels of inflammatory factors in serum were determined using liquid protein chip. The mRNA levels of chemotactic factors in lung tissues, leukocytes, and peritoneal macrophages were measured by qRT-PCR. The expression levels of IL-1α and HIF-1α were measured by immunocytochemistry. Results: Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. In addition, hydrogen significantly blocked LPS or H2O2-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Discussion: The results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol.https://www.frontiersin.org/articles/10.3389/fphar.2023.1138762/fullhydrogenHIF-1αIL-1αchemokineinflammation |
| spellingShingle | Youzhen Wei Youzhen Wei Youzhen Wei Kun Wang Yafang Zhang Yi Duan Yan Tian Hongling Yin Xuelian Fu Zuan Ma Jianjun Zhou Min Yu Qingbin Ni Wenjie Tang Wenjie Tang Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation Frontiers in Pharmacology hydrogen HIF-1α IL-1α chemokine inflammation |
| title | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
| title_full | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
| title_fullStr | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
| title_full_unstemmed | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
| title_short | Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation |
| title_sort | potent anti inflammatory responses role of hydrogen in il 1α dominated early phase systemic inflammation |
| topic | hydrogen HIF-1α IL-1α chemokine inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1138762/full |
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