Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i>
Diapause is a form of dormancy that organisms use to adapt to extreme environments by exhibiting developmental arrest. In the silkworm, <i>Bombyx mori</i>, diapause is thought to be elicited by diapause hormone (DH) signaling, which consists of interactions between DH and the DH receptor...
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MDPI AG
2022-09-01
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| author | Lijuan Chen Zhongjie Zhang Kai Chen Ye Yu Bo Hu Hongsheng Song Xiaojing Liu |
| author_facet | Lijuan Chen Zhongjie Zhang Kai Chen Ye Yu Bo Hu Hongsheng Song Xiaojing Liu |
| author_sort | Lijuan Chen |
| collection | DOAJ |
| description | Diapause is a form of dormancy that organisms use to adapt to extreme environments by exhibiting developmental arrest. In the silkworm, <i>Bombyx mori</i>, diapause is thought to be elicited by diapause hormone (DH) signaling, which consists of interactions between DH and the DH receptor (DHR). However, the steps downstream of the DH signaling pathway are largely unknown. In the present study, we directly injected synthesized DH into the female pupae of a multivoltine, non-diapausing strain at 36 h after pupation. We found that the mRNA level of <i>DHR</i> declined at 4 h and recovered at 12 h after the injection of DH. Thus, we sequenced the transcriptome of the ovaries at 4 h and 12 h after the injection of DH. We identified 60 and 221 differentially expressed genes at 4 h and 12 h after the injection, respectively. All DEGs were identified, relating to 20E-related genes, JH-related genes, cellular detoxification, ribosomal proteins, lipid metabolism, and epigenetic modifications. Eleven genes were selected from the above categories to verify the transcriptome data. The qRT-PCR and RNA-Seq expression patterns of the genes were consistent, which indicated the authenticity and reliability of the transcriptome data. This study dramatically expands upon our knowledge of gene expression variation at the early phase of DH release. |
| first_indexed | 2024-03-10T00:41:35Z |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:41:35Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-cc8e3a82a26d40b1aecb3a8390eced4f2023-11-23T15:07:27ZengMDPI AGBiology2079-77372022-09-01119131310.3390/biology11091313Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i>Lijuan Chen0Zhongjie Zhang1Kai Chen2Ye Yu3Bo Hu4Hongsheng Song5Xiaojing Liu6College of Life Sciences, Shanghai University, Shanghai 200444, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaCAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai 200032, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaCollege of Life Sciences, Shanghai University, Shanghai 200444, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaDiapause is a form of dormancy that organisms use to adapt to extreme environments by exhibiting developmental arrest. In the silkworm, <i>Bombyx mori</i>, diapause is thought to be elicited by diapause hormone (DH) signaling, which consists of interactions between DH and the DH receptor (DHR). However, the steps downstream of the DH signaling pathway are largely unknown. In the present study, we directly injected synthesized DH into the female pupae of a multivoltine, non-diapausing strain at 36 h after pupation. We found that the mRNA level of <i>DHR</i> declined at 4 h and recovered at 12 h after the injection of DH. Thus, we sequenced the transcriptome of the ovaries at 4 h and 12 h after the injection of DH. We identified 60 and 221 differentially expressed genes at 4 h and 12 h after the injection, respectively. All DEGs were identified, relating to 20E-related genes, JH-related genes, cellular detoxification, ribosomal proteins, lipid metabolism, and epigenetic modifications. Eleven genes were selected from the above categories to verify the transcriptome data. The qRT-PCR and RNA-Seq expression patterns of the genes were consistent, which indicated the authenticity and reliability of the transcriptome data. This study dramatically expands upon our knowledge of gene expression variation at the early phase of DH release.https://www.mdpi.com/2079-7737/11/9/1313silkwormdiapausediapause hormoneRNA sequencing |
| spellingShingle | Lijuan Chen Zhongjie Zhang Kai Chen Ye Yu Bo Hu Hongsheng Song Xiaojing Liu Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> Biology silkworm diapause diapause hormone RNA sequencing |
| title | Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> |
| title_full | Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> |
| title_fullStr | Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> |
| title_full_unstemmed | Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> |
| title_short | Transcriptional Dynamics Induced by Diapause Hormone in the Silkworm, <i>Bombyx mori</i> |
| title_sort | transcriptional dynamics induced by diapause hormone in the silkworm i bombyx mori i |
| topic | silkworm diapause diapause hormone RNA sequencing |
| url | https://www.mdpi.com/2079-7737/11/9/1313 |
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