SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY
This paper presents the results of studies of seed copolymerization of glycidyl methacrylate with ethylene glycol poly(glycidyl methacrylate) on seed particles in order to obtain partially crosslinked polymer microspheres with a diameter of about 3.5 microns for use in immunochemical reactions, as c...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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MIREA - Russian Technological University
2017-08-01
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| Series: | Тонкие химические технологии |
| Subjects: | |
| Online Access: | https://www.finechem-mirea.ru/jour/article/view/107 |
| _version_ | 1826565743878078464 |
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| author | A. V. Bakhtina A. A. Sivaev S. M. Levachev S. A. Gusev N. A. Lobanova M. A. Lazov I. A. Gritskova |
| author_facet | A. V. Bakhtina A. A. Sivaev S. M. Levachev S. A. Gusev N. A. Lobanova M. A. Lazov I. A. Gritskova |
| author_sort | A. V. Bakhtina |
| collection | DOAJ |
| description | This paper presents the results of studies of seed copolymerization of glycidyl methacrylate with ethylene glycol poly(glycidyl methacrylate) on seed particles in order to obtain partially crosslinked polymer microspheres with a diameter of about 3.5 microns for use in immunochemical reactions, as carriers of bioligands. The physico-chemical properties of polymeric microspheres obtained under different conditions were studied by the following methods: the diameters of the particles - by electron scanning and light microscopy; the wetting angle - bу the method of "lying droplets"; the sedimentation velocity - by the macromethod in a capillary tube by the movement of the (polymer slurry)/water phase boundary (buffer solution); the zeta-potential - by dynamic light scattering. Having studied the physical-chemical properties of all polymer slurries we concluded that the optimal particles to create on the basis of their diagnostic test systems with high sensitivity working on the principle of latex agglutination reaction are poly(glycidyl methacrylate) particles with equal percentage of glycidyl methacrylate and ethylene glycol dimethacrylate aminated with hexamethylendiamine in the environment of n-propanol, because they have a sufficient number of amino groups and do not lose their sedimentation properties after all stages of the synthesis. |
| first_indexed | 2024-04-10T03:29:04Z |
| format | Article |
| id | doaj.art-cc9173f746ab4ffeb86c8996412dbb62 |
| institution | Directory Open Access Journal |
| issn | 2410-6593 2686-7575 |
| language | Russian |
| last_indexed | 2025-03-14T10:40:37Z |
| publishDate | 2017-08-01 |
| publisher | MIREA - Russian Technological University |
| record_format | Article |
| series | Тонкие химические технологии |
| spelling | doaj.art-cc9173f746ab4ffeb86c8996412dbb622025-03-02T10:57:34ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752017-08-01124758410.32362/2410-6593-2017-12-4-75-84107SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGYA. V. Bakhtina0A. A. Sivaev1S. M. Levachev2S. A. Gusev3N. A. Lobanova4M. A. Lazov5I. A. Gritskova6Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)M.V. Lomonosov Moscow State UniversityFederal Scientific-Clinical Center of Physico-Chemical Medicine FMBA of RussiaMoscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)Moscow Technological University (M.V. Lomonosov Institute of Fine Chemical Technologies)This paper presents the results of studies of seed copolymerization of glycidyl methacrylate with ethylene glycol poly(glycidyl methacrylate) on seed particles in order to obtain partially crosslinked polymer microspheres with a diameter of about 3.5 microns for use in immunochemical reactions, as carriers of bioligands. The physico-chemical properties of polymeric microspheres obtained under different conditions were studied by the following methods: the diameters of the particles - by electron scanning and light microscopy; the wetting angle - bу the method of "lying droplets"; the sedimentation velocity - by the macromethod in a capillary tube by the movement of the (polymer slurry)/water phase boundary (buffer solution); the zeta-potential - by dynamic light scattering. Having studied the physical-chemical properties of all polymer slurries we concluded that the optimal particles to create on the basis of their diagnostic test systems with high sensitivity working on the principle of latex agglutination reaction are poly(glycidyl methacrylate) particles with equal percentage of glycidyl methacrylate and ethylene glycol dimethacrylate aminated with hexamethylendiamine in the environment of n-propanol, because they have a sufficient number of amino groups and do not lose their sedimentation properties after all stages of the synthesis.https://www.finechem-mirea.ru/jour/article/view/107diagnostic test systemsseed copolymerizationlatex agglutinationpoly(glycidylmethacrylate)physico-chemical properties |
| spellingShingle | A. V. Bakhtina A. A. Sivaev S. M. Levachev S. A. Gusev N. A. Lobanova M. A. Lazov I. A. Gritskova SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY Тонкие химические технологии diagnostic test systems seed copolymerization latex agglutination poly(glycidylmethacrylate) physico-chemical properties |
| title | SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY |
| title_full | SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY |
| title_fullStr | SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY |
| title_full_unstemmed | SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY |
| title_short | SYNTHESIS OF AMINE-CONTAINING POLYMERIC MICROSPHERES BY SEED COPOLYMERIZATION FOR APPLICATIONS IN BIOTECHNOLOGY |
| title_sort | synthesis of amine containing polymeric microspheres by seed copolymerization for applications in biotechnology |
| topic | diagnostic test systems seed copolymerization latex agglutination poly(glycidylmethacrylate) physico-chemical properties |
| url | https://www.finechem-mirea.ru/jour/article/view/107 |
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