Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population
To investigate the dependence of HDL quantitative trait loci (QTL) on sex and diet, we generated a large intercross population of mice from parental strains SM/J and NZB/BlNJ. We measured HDL levels in progeny fed a chow diet and measured them again after 6, 12, and 16 weeks of feeding a high-fat, h...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520318289 |
| _version_ | 1819072372380008448 |
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| author | Ron Korstanje Renhua Li Timothy Howard Peter Kelmenson Jan Marshall Beverly Paigen Gary Churchill |
| author_facet | Ron Korstanje Renhua Li Timothy Howard Peter Kelmenson Jan Marshall Beverly Paigen Gary Churchill |
| author_sort | Ron Korstanje |
| collection | DOAJ |
| description | To investigate the dependence of HDL quantitative trait loci (QTL) on sex and diet, we generated a large intercross population of mice from parental strains SM/J and NZB/BlNJ. We measured HDL levels in progeny fed a chow diet and measured them again after 6, 12, and 16 weeks of feeding a high-fat, high-cholesterol diet. QTL analysis was performed on the 260 female and 253 male F2 progeny. A total of 13 significant QTL were found. Four QTL were specific to female mice: Hdlq23 (Chr 6, 26 cM), Hdlq26 (Chr 10, 70 cM), Hdlq27 (Chr 15, 48 cM), and Hdlq32 (Chr 19, 40 cM). One significant QTL was specific to male mice: Hdlq29 (Chr 17, 36 cM). In addition, several QTL were found to have effects that were dependent on diet. Sex- and diet-dependent effects were characterized using a linear model-based genome scan method that avoids the potential pitfalls of subdivided data analysis.The dependence of QTL effects on sex suggests an important role for the sex hormones in HDL regulation. We recommend that sex should be explicitly accounted for in future studies in the genetics of HDL regulation in both mice and humans. |
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| format | Article |
| id | doaj.art-cc95b20633f2440b86df718a40bacc8f |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-21T17:36:41Z |
| publishDate | 2004-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-cc95b20633f2440b86df718a40bacc8f2022-12-21T18:55:45ZengElsevierJournal of Lipid Research0022-22752004-05-01455881888Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross populationRon Korstanje0Renhua Li1Timothy Howard2Peter Kelmenson3Jan Marshall4Beverly Paigen5Gary Churchill6The Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCThe Jackson Laboratory, 600 Main St., Bar Harbor, ME 04609; Wake Forest University, Winston-Salem, NCTo investigate the dependence of HDL quantitative trait loci (QTL) on sex and diet, we generated a large intercross population of mice from parental strains SM/J and NZB/BlNJ. We measured HDL levels in progeny fed a chow diet and measured them again after 6, 12, and 16 weeks of feeding a high-fat, high-cholesterol diet. QTL analysis was performed on the 260 female and 253 male F2 progeny. A total of 13 significant QTL were found. Four QTL were specific to female mice: Hdlq23 (Chr 6, 26 cM), Hdlq26 (Chr 10, 70 cM), Hdlq27 (Chr 15, 48 cM), and Hdlq32 (Chr 19, 40 cM). One significant QTL was specific to male mice: Hdlq29 (Chr 17, 36 cM). In addition, several QTL were found to have effects that were dependent on diet. Sex- and diet-dependent effects were characterized using a linear model-based genome scan method that avoids the potential pitfalls of subdivided data analysis.The dependence of QTL effects on sex suggests an important role for the sex hormones in HDL regulation. We recommend that sex should be explicitly accounted for in future studies in the genetics of HDL regulation in both mice and humans.http://www.sciencedirect.com/science/article/pii/S0022227520318289diethigh density lipoproteinmicesex |
| spellingShingle | Ron Korstanje Renhua Li Timothy Howard Peter Kelmenson Jan Marshall Beverly Paigen Gary Churchill Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population Journal of Lipid Research diet high density lipoprotein mice sex |
| title | Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population |
| title_full | Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population |
| title_fullStr | Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population |
| title_full_unstemmed | Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population |
| title_short | Influence of sex and diet on quantitative trait loci for HDL cholesterol levels in an SM/J by NZB/BlNJ intercross population |
| title_sort | influence of sex and diet on quantitative trait loci for hdl cholesterol levels in an sm j by nzb blnj intercross population |
| topic | diet high density lipoprotein mice sex |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520318289 |
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