The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch
The combustion oxidation of nitrocellulose at 300 o C was employed to modify activated carbon by the introduction of oxygen-containing complexes onto its surface. The pore structure, elemental composition and chemical nature of the functional groups attached to the surface of the activated carbon we...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2008-05-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/026361708786934433 |
| _version_ | 1797284044113707008 |
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| author | Chan Liu Rengui Guan Wenzhong Shen Zhijie Li |
| author_facet | Chan Liu Rengui Guan Wenzhong Shen Zhijie Li |
| author_sort | Chan Liu |
| collection | DOAJ |
| description | The combustion oxidation of nitrocellulose at 300 o C was employed to modify activated carbon by the introduction of oxygen-containing complexes onto its surface. The pore structure, elemental composition and chemical nature of the functional groups attached to the surface of the activated carbon were investigated after such oxidation. The results obtained show that oxygen-containing complexes may be introduced efficiently via the combustion of nitrocellulose, with the adsorption capacity of the resulting activated carbon towards α-amylase being enhanced due to interaction between the amino groups of α-amylase and the oxygen-containing surface groups. Thus, oxidized activated carbons with α-amylase immobilized on their surfaces exhibited a hydrolysis activity greater than 63% (based on free α-amylase) after five runs, while the hydrolysis activity of α-amylase immobilized on the original activated carbon was only 15% after two runs. A simple method for introducing oxygen-containing complexes onto the surface of activated carbon through the use of nitrocellulose combustion is described in the present work. The resulting oxidized activated carbon may be used as a support for the immobilization of α-amylase, with the immobilized α-amylase exhibiting a high hydrolysis activity even after repeated use. |
| first_indexed | 2024-03-07T17:40:18Z |
| format | Article |
| id | doaj.art-cc99197ddfa6438a8be2835623887ae5 |
| institution | Directory Open Access Journal |
| issn | 0263-6174 2048-4038 |
| language | English |
| last_indexed | 2024-03-07T17:40:18Z |
| publishDate | 2008-05-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj.art-cc99197ddfa6438a8be2835623887ae52024-03-02T16:04:20ZengSAGE PublicationsAdsorption Science & Technology0263-61742048-40382008-05-012610.1260/026361708786934433The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards StarchChan Liu0Rengui Guan1Wenzhong Shen2Zhijie Li3 Science and Engineering College of Chemistry and Biology, Yantai University, Yantai 264005, P. R. China Science and Engineering College of Chemistry and Biology, Yantai University, Yantai 264005, P. R. China State Key Laboratory of Heavy Oil, China University of Petroleum, Dongying, Shandong 257061, P. R. China Department of Applied Physics, University of Electronic Science and Technology of China, Chengdu 610041, P. R. ChinaThe combustion oxidation of nitrocellulose at 300 o C was employed to modify activated carbon by the introduction of oxygen-containing complexes onto its surface. The pore structure, elemental composition and chemical nature of the functional groups attached to the surface of the activated carbon were investigated after such oxidation. The results obtained show that oxygen-containing complexes may be introduced efficiently via the combustion of nitrocellulose, with the adsorption capacity of the resulting activated carbon towards α-amylase being enhanced due to interaction between the amino groups of α-amylase and the oxygen-containing surface groups. Thus, oxidized activated carbons with α-amylase immobilized on their surfaces exhibited a hydrolysis activity greater than 63% (based on free α-amylase) after five runs, while the hydrolysis activity of α-amylase immobilized on the original activated carbon was only 15% after two runs. A simple method for introducing oxygen-containing complexes onto the surface of activated carbon through the use of nitrocellulose combustion is described in the present work. The resulting oxidized activated carbon may be used as a support for the immobilization of α-amylase, with the immobilized α-amylase exhibiting a high hydrolysis activity even after repeated use.https://doi.org/10.1260/026361708786934433 |
| spellingShingle | Chan Liu Rengui Guan Wenzhong Shen Zhijie Li The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch Adsorption Science & Technology |
| title | The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch |
| title_full | The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch |
| title_fullStr | The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch |
| title_full_unstemmed | The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch |
| title_short | The Adsorption of α-Amylase onto Activated Carbon Modified by Nitrocellulose Combustion and its Resulting Hydrolysis Activity towards Starch |
| title_sort | adsorption of α amylase onto activated carbon modified by nitrocellulose combustion and its resulting hydrolysis activity towards starch |
| url | https://doi.org/10.1260/026361708786934433 |
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