Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae
Microalgae are considered as ideal cell factories for producing natural carotenoids which display favorable biological activities. As the most important abiotic factor, light not only provides energy for photosynthetic metabolism, but also regulates numerous biological processes. Blue light is the m...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1083387/full |
| _version_ | 1828089486606598144 |
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| author | Zhongyi Zhang Tianli Han Jikang Sui Hui Wang Hui Wang |
| author_facet | Zhongyi Zhang Tianli Han Jikang Sui Hui Wang Hui Wang |
| author_sort | Zhongyi Zhang |
| collection | DOAJ |
| description | Microalgae are considered as ideal cell factories for producing natural carotenoids which display favorable biological activities. As the most important abiotic factor, light not only provides energy for photosynthetic metabolism, but also regulates numerous biological processes. Blue light is the main wavelength of light that can travel through water. Previous studies have shown that blue light triggered carotenoid accumulation in several microalgae species, but the molecular mechanism remains unclear. Cryptochromes were blue-light-absorbing photoreceptors that have been found in all studied algal genomes. In this study, several different types of cryptochrome genes were cloned from Haematococcus pluvialis and Phaeodactylum tricornutum. Among them, cryptochrome genes HpCRY4 from H. pluvialis and PtCPF1 from P. tricornutum were upregulated under blue light treatment, in correlation with the increase of astaxanthin and fucoxanthin contents. Besides, heterologous expression and gene knockout was performed to verify the function of HpCRY4 and PtCPF1 in regulating carotenoid biosynthesis in microalgae. These results indicate that carotenoid biosynthesis in microalgae promoted by blue light was mediated by cryptochromes as photoreceptors. |
| first_indexed | 2024-04-11T05:39:33Z |
| format | Article |
| id | doaj.art-cc9a8075b7014fbbbc5f945be953fe8e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T05:39:33Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-cc9a8075b7014fbbbc5f945be953fe8e2022-12-22T07:19:16ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10833871083387Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgaeZhongyi Zhang0Tianli Han1Jikang Sui2Hui Wang3Hui Wang4Solar Energy Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaSolar Energy Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaSolar Energy Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaSolar Energy Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences (CAS), Qingdao, ChinaShandong Energy Research Institute, Qingdao, ChinaMicroalgae are considered as ideal cell factories for producing natural carotenoids which display favorable biological activities. As the most important abiotic factor, light not only provides energy for photosynthetic metabolism, but also regulates numerous biological processes. Blue light is the main wavelength of light that can travel through water. Previous studies have shown that blue light triggered carotenoid accumulation in several microalgae species, but the molecular mechanism remains unclear. Cryptochromes were blue-light-absorbing photoreceptors that have been found in all studied algal genomes. In this study, several different types of cryptochrome genes were cloned from Haematococcus pluvialis and Phaeodactylum tricornutum. Among them, cryptochrome genes HpCRY4 from H. pluvialis and PtCPF1 from P. tricornutum were upregulated under blue light treatment, in correlation with the increase of astaxanthin and fucoxanthin contents. Besides, heterologous expression and gene knockout was performed to verify the function of HpCRY4 and PtCPF1 in regulating carotenoid biosynthesis in microalgae. These results indicate that carotenoid biosynthesis in microalgae promoted by blue light was mediated by cryptochromes as photoreceptors.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1083387/fullcryptochromeblue lightcarotenoid biosynthesisPhaeodactylum tricornutumHaematococcus pluvialis |
| spellingShingle | Zhongyi Zhang Tianli Han Jikang Sui Hui Wang Hui Wang Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae Frontiers in Microbiology cryptochrome blue light carotenoid biosynthesis Phaeodactylum tricornutum Haematococcus pluvialis |
| title | Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae |
| title_full | Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae |
| title_fullStr | Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae |
| title_full_unstemmed | Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae |
| title_short | Cryptochrome-mediated blue-light signal contributes to carotenoids biosynthesis in microalgae |
| title_sort | cryptochrome mediated blue light signal contributes to carotenoids biosynthesis in microalgae |
| topic | cryptochrome blue light carotenoid biosynthesis Phaeodactylum tricornutum Haematococcus pluvialis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1083387/full |
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