Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper
Abstract Background The B3 gene family, one of the largest plant-specific transcription factors, plays important roles in plant growth, seed development, and hormones. However, the B3 gene family, especially the REM subfamily, has not been systematically and functionally studied. Results In this stu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-04897-w |
| _version_ | 1797247429807964160 |
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| author | Young-Soo Park Hye Jeong Cho Seungill Kim |
| author_facet | Young-Soo Park Hye Jeong Cho Seungill Kim |
| author_sort | Young-Soo Park |
| collection | DOAJ |
| description | Abstract Background The B3 gene family, one of the largest plant-specific transcription factors, plays important roles in plant growth, seed development, and hormones. However, the B3 gene family, especially the REM subfamily, has not been systematically and functionally studied. Results In this study, we performed genome-wide re-annotation of B3 genes in five Solanaceae plants, Arabidopsis thaliana, and Oryza sativa, and finally predicted 1,039 B3 genes, including 231 (22.2%) newly annotated genes. We found a striking abundance of REM genes in pepper species (Capsicum annuum, Capsicum baccatum, and Capsicum chinense). Comparative motif analysis revealed that REM and other subfamilies (ABI3/VP1, ARF, RAV, and HSI) consist of different amino acids. We verified that the large number of REM genes in pepper were included in the specific subgroup (G8) through the phylogenetic analysis. Chromosome location and evolutionary analyses suggested that the G8 subgroup genes evolved mainly via a pepper-specific recent tandem duplication on chromosomes 1 and 3 after speciation between pepper and other Solanaceae. RNA-seq analyses suggested the potential functions of REM genes under salt, heat, cold, and mannitol stress conditions in pepper (C. annuum). Conclusions Our study provides evolutionary and functional insights into the REM gene family in pepper. |
| first_indexed | 2024-04-24T19:58:34Z |
| format | Article |
| id | doaj.art-cc9b4595c8a844488fd10b62fd18200f |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-24T19:58:34Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-cc9b4595c8a844488fd10b62fd18200f2024-03-24T12:14:59ZengBMCBMC Plant Biology1471-22292024-03-0124111310.1186/s12870-024-04897-wIdentification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepperYoung-Soo Park0Hye Jeong Cho1Seungill Kim2Department of Environmental Horticulture, University of SeoulDepartment of Environmental Horticulture, University of SeoulDepartment of Environmental Horticulture, University of SeoulAbstract Background The B3 gene family, one of the largest plant-specific transcription factors, plays important roles in plant growth, seed development, and hormones. However, the B3 gene family, especially the REM subfamily, has not been systematically and functionally studied. Results In this study, we performed genome-wide re-annotation of B3 genes in five Solanaceae plants, Arabidopsis thaliana, and Oryza sativa, and finally predicted 1,039 B3 genes, including 231 (22.2%) newly annotated genes. We found a striking abundance of REM genes in pepper species (Capsicum annuum, Capsicum baccatum, and Capsicum chinense). Comparative motif analysis revealed that REM and other subfamilies (ABI3/VP1, ARF, RAV, and HSI) consist of different amino acids. We verified that the large number of REM genes in pepper were included in the specific subgroup (G8) through the phylogenetic analysis. Chromosome location and evolutionary analyses suggested that the G8 subgroup genes evolved mainly via a pepper-specific recent tandem duplication on chromosomes 1 and 3 after speciation between pepper and other Solanaceae. RNA-seq analyses suggested the potential functions of REM genes under salt, heat, cold, and mannitol stress conditions in pepper (C. annuum). Conclusions Our study provides evolutionary and functional insights into the REM gene family in pepper.https://doi.org/10.1186/s12870-024-04897-wB3Transcription factorsSolanaceaeRe-annotationREMPepper |
| spellingShingle | Young-Soo Park Hye Jeong Cho Seungill Kim Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper BMC Plant Biology B3 Transcription factors Solanaceae Re-annotation REM Pepper |
| title | Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper |
| title_full | Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper |
| title_fullStr | Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper |
| title_full_unstemmed | Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper |
| title_short | Identification and expression analyses of B3 genes reveal lineage-specific evolution and potential roles of REM genes in pepper |
| title_sort | identification and expression analyses of b3 genes reveal lineage specific evolution and potential roles of rem genes in pepper |
| topic | B3 Transcription factors Solanaceae Re-annotation REM Pepper |
| url | https://doi.org/10.1186/s12870-024-04897-w |
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