A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression
<p>Abstract</p> <p>Background</p> <p>The amide class compound, 3, 4-dichloropropionanilide (DCPA) is known to affect multiple signaling pathways in lymphocyte and macrophage including the inhibition of NF-κB ability. However, little is known about the effect of DCPA in...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-08-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/6/204 |
| _version_ | 1811295795475906560 |
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| author | Schafer Rosana Liu Ling-Zhi Jiang Bing-Hua Flynn Daniel C Barnett John B |
| author_facet | Schafer Rosana Liu Ling-Zhi Jiang Bing-Hua Flynn Daniel C Barnett John B |
| author_sort | Schafer Rosana |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The amide class compound, 3, 4-dichloropropionanilide (DCPA) is known to affect multiple signaling pathways in lymphocyte and macrophage including the inhibition of NF-κB ability. However, little is known about the effect of DCPA in cancer cells. Hypoxia-inducible factor 1 (HIF-1) regulates the expression of many genes including vascular endothelial growth factor (VEGF), heme oxygenase 1, inducible nitric oxide synthase, aldolase, enolase, and lactate dehydrogenase A. HIF-1 expression is associated with tumorigenesis and angiogenesis.</p> <p>Methods</p> <p>We used Transwell assay to study cell migration, and used immunoblotting to study specific protein expression in the cells.</p> <p>Results</p> <p>In this report, we demonstrate that DCPA inhibited the migration and proliferation of DU145 and PC-3 prostate cancer cells induced by serum, insulin, and insulin-like growth factor I (IGF-I). We found that DCPA inhibited HIF-1 expression in a subunit-specific manner in these cancer cell lines induced by serum and growth factors, and decreased HIF-1α expression by affecting its protein stability.</p> <p>Conclusion</p> <p>DCPA can inhibit prostate cancer cell migration, proliferation, and HIF-1α expression, suggesting that DCPA could be potentially used for therapeutic purpose for prostate cancer in the future.</p> |
| first_indexed | 2024-04-13T05:37:51Z |
| format | Article |
| id | doaj.art-cc9c2208965c488d900ab4c24cb1b207 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T05:37:51Z |
| publishDate | 2006-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-cc9c2208965c488d900ab4c24cb1b2072022-12-22T03:00:12ZengBMCBMC Cancer1471-24072006-08-016120410.1186/1471-2407-6-204A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expressionSchafer RosanaLiu Ling-ZhiJiang Bing-HuaFlynn Daniel CBarnett John B<p>Abstract</p> <p>Background</p> <p>The amide class compound, 3, 4-dichloropropionanilide (DCPA) is known to affect multiple signaling pathways in lymphocyte and macrophage including the inhibition of NF-κB ability. However, little is known about the effect of DCPA in cancer cells. Hypoxia-inducible factor 1 (HIF-1) regulates the expression of many genes including vascular endothelial growth factor (VEGF), heme oxygenase 1, inducible nitric oxide synthase, aldolase, enolase, and lactate dehydrogenase A. HIF-1 expression is associated with tumorigenesis and angiogenesis.</p> <p>Methods</p> <p>We used Transwell assay to study cell migration, and used immunoblotting to study specific protein expression in the cells.</p> <p>Results</p> <p>In this report, we demonstrate that DCPA inhibited the migration and proliferation of DU145 and PC-3 prostate cancer cells induced by serum, insulin, and insulin-like growth factor I (IGF-I). We found that DCPA inhibited HIF-1 expression in a subunit-specific manner in these cancer cell lines induced by serum and growth factors, and decreased HIF-1α expression by affecting its protein stability.</p> <p>Conclusion</p> <p>DCPA can inhibit prostate cancer cell migration, proliferation, and HIF-1α expression, suggesting that DCPA could be potentially used for therapeutic purpose for prostate cancer in the future.</p>http://www.biomedcentral.com/1471-2407/6/204 |
| spellingShingle | Schafer Rosana Liu Ling-Zhi Jiang Bing-Hua Flynn Daniel C Barnett John B A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression BMC Cancer |
| title | A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression |
| title_full | A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression |
| title_fullStr | A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression |
| title_full_unstemmed | A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression |
| title_short | A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression |
| title_sort | novel role for 3 4 dichloropropionanilide dcpa in the inhibition of prostate cancer cell migration proliferation and hypoxia inducible factor 1alpha expression |
| url | http://www.biomedcentral.com/1471-2407/6/204 |
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