Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model
Imaging techniques for diagnosing muscle atrophy and sarcopenia remain insufficient, although various advanced diagnostic methods have been established. We explored the feasibility of <sup>18</sup>F-fluorocholine (<sup>18</sup>F-FCH) positron emission tomography/computed tomo...
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MDPI AG
2022-05-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/5/1274 |
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| author | Sun Mi Park Jisu Kim Suji Baek Joo-Yeong Jeon Sang Ju Lee Seo Young Kang Min Young Yoo Hai-Jeon Yoon Seung Hae Kwon Kiwon Lim Seung Jun Oh Bom Sahn Kim Kang Pa Lee Byung Seok Moon |
| author_facet | Sun Mi Park Jisu Kim Suji Baek Joo-Yeong Jeon Sang Ju Lee Seo Young Kang Min Young Yoo Hai-Jeon Yoon Seung Hae Kwon Kiwon Lim Seung Jun Oh Bom Sahn Kim Kang Pa Lee Byung Seok Moon |
| author_sort | Sun Mi Park |
| collection | DOAJ |
| description | Imaging techniques for diagnosing muscle atrophy and sarcopenia remain insufficient, although various advanced diagnostic methods have been established. We explored the feasibility of <sup>18</sup>F-fluorocholine (<sup>18</sup>F-FCH) positron emission tomography/computed tomography (PET/CT) for evaluating skeletal muscle atrophy, as an imaging technique that tracks choline level changes in muscles. Cell uptake in L6 cells by <sup>18</sup>F-FCH was performed in a complete medium containing serum (untreated group, UN) and a serum-free medium (starved group, ST). Small-animal-dedicated PET/CT imaging with <sup>18</sup>F-FCH was examined in in-vivo models with rats that were starved for 2 days to cause muscle atrophy. After the hind limbs were dissected, starvation-induced in-vivo models were anatomically confirmed by reverse-transcription polymerase chain reaction to evaluate the expression levels of the atrophy markers muscle RING-finger protein-1 (MuRF-1) and atrogin-1. <sup>18</sup>F-FCH uptake was lower in the starvation-induced cells than in the untreated group, and in-vivo PET uptake also revealed a similar tendency (the average standardized uptake value (SUV<sub>mean</sub>) = 0.26 ± 0.06 versus 0.37 ± 0.07, respectively). Furthermore, the expression levels of MuRF-1 and atrogin-1 mRNA were significantly increased in the starvation-induced muscle atrophy of rats compared to the untreated group. <sup>18</sup>F-FCH PET/CT may be a promising tool for diagnosing skeletal muscle atrophy. |
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| last_indexed | 2024-03-10T03:02:18Z |
| publishDate | 2022-05-01 |
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| series | Diagnostics |
| spelling | doaj.art-cca16306beee4ec0ae5bde6392cb6f3d2023-11-23T10:41:51ZengMDPI AGDiagnostics2075-44182022-05-01125127410.3390/diagnostics12051274Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat ModelSun Mi Park0Jisu Kim1Suji Baek2Joo-Yeong Jeon3Sang Ju Lee4Seo Young Kang5Min Young Yoo6Hai-Jeon Yoon7Seung Hae Kwon8Kiwon Lim9Seung Jun Oh10Bom Sahn Kim11Kang Pa Lee12Byung Seok Moon13Department of Nuclear Medicine, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul 07804, KoreaPhysical Activity and Performance Institute, Konkuk University, Seoul 05029, KoreaResearch and Development Center, UMUST R&D Corporation, Seoul 01411, KoreaSeoul Center, Korean Basic Science Institute, Seoul 02841, KoreaDepartment of Nuclear Medicine, College of Medicine, Asan Medical Center, University of Ulsan, Seoul 05505, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul 07804, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Mokdong Hospital, Ewha Womans University, Seoul 07985, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Mokdong Hospital, Ewha Womans University, Seoul 07985, KoreaSeoul Center, Korean Basic Science Institute, Seoul 02841, KoreaPhysical Activity and Performance Institute, Konkuk University, Seoul 05029, KoreaDepartment of Nuclear Medicine, College of Medicine, Asan Medical Center, University of Ulsan, Seoul 05505, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul 07804, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul 07804, KoreaDepartment of Nuclear Medicine, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul 07804, KoreaImaging techniques for diagnosing muscle atrophy and sarcopenia remain insufficient, although various advanced diagnostic methods have been established. We explored the feasibility of <sup>18</sup>F-fluorocholine (<sup>18</sup>F-FCH) positron emission tomography/computed tomography (PET/CT) for evaluating skeletal muscle atrophy, as an imaging technique that tracks choline level changes in muscles. Cell uptake in L6 cells by <sup>18</sup>F-FCH was performed in a complete medium containing serum (untreated group, UN) and a serum-free medium (starved group, ST). Small-animal-dedicated PET/CT imaging with <sup>18</sup>F-FCH was examined in in-vivo models with rats that were starved for 2 days to cause muscle atrophy. After the hind limbs were dissected, starvation-induced in-vivo models were anatomically confirmed by reverse-transcription polymerase chain reaction to evaluate the expression levels of the atrophy markers muscle RING-finger protein-1 (MuRF-1) and atrogin-1. <sup>18</sup>F-FCH uptake was lower in the starvation-induced cells than in the untreated group, and in-vivo PET uptake also revealed a similar tendency (the average standardized uptake value (SUV<sub>mean</sub>) = 0.26 ± 0.06 versus 0.37 ± 0.07, respectively). Furthermore, the expression levels of MuRF-1 and atrogin-1 mRNA were significantly increased in the starvation-induced muscle atrophy of rats compared to the untreated group. <sup>18</sup>F-FCH PET/CT may be a promising tool for diagnosing skeletal muscle atrophy.https://www.mdpi.com/2075-4418/12/5/1274skeletal muscle atrophy<sup>18</sup>F-Fluorocholinepositron emission tomographyMuRF-1atrogin-1 |
| spellingShingle | Sun Mi Park Jisu Kim Suji Baek Joo-Yeong Jeon Sang Ju Lee Seo Young Kang Min Young Yoo Hai-Jeon Yoon Seung Hae Kwon Kiwon Lim Seung Jun Oh Bom Sahn Kim Kang Pa Lee Byung Seok Moon Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model Diagnostics skeletal muscle atrophy <sup>18</sup>F-Fluorocholine positron emission tomography MuRF-1 atrogin-1 |
| title | Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model |
| title_full | Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model |
| title_fullStr | Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model |
| title_full_unstemmed | Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model |
| title_short | Feasibility of <sup>18</sup>F-Fluorocholine PET for Evaluating Skeletal Muscle Atrophy in a Starved Rat Model |
| title_sort | feasibility of sup 18 sup f fluorocholine pet for evaluating skeletal muscle atrophy in a starved rat model |
| topic | skeletal muscle atrophy <sup>18</sup>F-Fluorocholine positron emission tomography MuRF-1 atrogin-1 |
| url | https://www.mdpi.com/2075-4418/12/5/1274 |
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